Stable reagent kit of ethanol determination liquid

The technology of a kit and a composite stabilizer is applied in the field of medical testing and determination, and can solve the problems of unstable alcohol dehydrogenase, unstable coenzyme I, unable to meet the needs of clinical testing, etc., and achieve the effect of avoiding inhibition.

Active Publication Date: 2012-06-13
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The enzyme analysis method has the advantages of high specificity, simple and quick operation, accurate and safe, and automatic analysis. However, due to the extreme instability of alcohol dehydrogenase, high concentrations can only exist stably for a few days at 5°C in near-neutral pure water. It is unstable in acidic (pH lower than 6.0) and alkaline (pH greater than 8.5) solutions, and heavy metals and commonly used reaction accelerators such as semicarbazide hydrochloride, glycine, etc. have obvious inhibitory effects on it; in addition, the reagents used Coenzyme I is unstable under this reaction condition; therefore, the reagent must be made into a dry powder reagent for long-term storage stability, and it must be used as soon as possible after reconstitution, otherwise, alcohol dehydrogenase and coenzyme I will degrade faster during storage. The performance of the reagent will have a great impact and cannot meet the needs of clinical testing

Method used

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  • Stable reagent kit of ethanol determination liquid
  • Stable reagent kit of ethanol determination liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The components and concentrations of reagent 1 are:

[0041] Sodium Phosphate Buffer (pH 8.5) 0.1 M

[0042] Semicarbazide Hydrochloride 2 g / L

[0043] Tween 20 1 g / L

[0044] Proclin300 0.1g / L;

[0045] The components and concentrations of reagent 2 are:

[0046] Tris hydrochloride buffer (pH 6.0) 0.05 M

[0047] Coenzyme I 0.5 g / L

[0048] Alcohol dehydrogenase 20 KU / L

[0049] Trehalose (compound stabilizer) 10 g / L

[0050] ES-W03 (compound stabilizer) 10 g / L

[0051] Mercaptoethanol (activator) 2 g / L

[0052] Proclin300 0.2 g / L.

[0053] The preparation method of Reagent 1 and Reagent 2 is a conventional method, that is, the components of Reagent 1 and Reagent 2 are added to distilled water and then mixed and stirred evenly.

Embodiment 2

[0055] The components and concentrations of reagent 1 are:

[0056] Tris hydrochloride buffer (pH 9.5) 0.05 M

[0057] Diglycopeptide 5 g / L

[0058] Tween 80 10 g / L

[0059] Proclin300 0.2g / L;

[0060] The components and concentrations of reagent 2 are:

[0061] 3-Trimethylolmethylamine-2-hydroxypropanesulfonic acid buffer (pH 6.5) 0.1 M

[0062] Coenzyme I 5 g / L

[0063] Alcohol dehydrogenase 50 KU / L

[0064] Cysteine ​​(compound stabilizer) 5 g / L

[0065] ES-W07 (compound stabilizer) 1 g / L

[0066] Dimercaptothreitol (activator) 10 g / L

[0067] Sodium azide 0.1 g / L.

[0068] The preparation method of the kit in Example 2 is the same as in Example 1.

Embodiment 3

[0070] The components and concentrations of reagent 1 are:

[0071] 4-Hydroxyethylpiperazine propanesulfonic acid buffer (pH 9.5) 0.1 M

[0072] Glycine 10 g / L

[0073] Bridger-35 5 g / L

[0074] Sodium azide 0.1 g / L

[0075] The components and concentrations of reagent 2 are:

[0076] Hydroxyethylpiperazine ethylsulfuric acid buffer (pH 7.0) 0.1 M

[0077] Coenzyme I 5 g / L

[0078] Alcohol dehydrogenase 50 KU / L

[0079] Glutathione (compound stabilizer) 2 g / L

[0080] ES-W08 (compound stabilizer) 2 g / L

[0081] Cysteine ​​(activator) 10 g / L

[0082] Disodium oxalate tetraacetate (activator) 1 g / L

[0083] Proclin300 0.1 g / L.

[0084] Example 3 The preparation method of the kit is the same as that in Example 1.

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Abstract

The invention discloses a stable reagent kit of ethanol determination liquid, which is a liquid double reagent formed by reagent I and reagent II, wherein the reagent I includes buffer solution (pH8.5-9.5)0.05-0.5M, reaction promoter 1-200g / L, stabilizer 0.1-10g / L, and preservative 0.1-10g / L; and reagent II includes buffer solution (pH6.0-7.0)0.05-0.5M, beta-coenzymeI 0.1-10g / L, alcohol dehydrogenase 0.2-200KU / L, compound stabilizer 0.1-10g / L, activator 0.1g-100g / L, and preservative 0.1-10g / L. The invention has the advantages that the reagent kit is highly accurate, precise and stable, can be stably stored at least for 12 months away from the light, and can fully satisfy the requirements of clinic detection.

Description

technical field [0001] The invention belongs to the technical field of medical examination and determination, and in particular relates to a stable liquid kit for ethanol determination. Background technique [0002] Ethanol (alcohol) exists in most alcoholic beverages, and its metabolic rate mainly depends on the content of enzymes in the body, with large individual differences. As we all know, drinking a small amount of alcohol can promote blood circulation, but a large amount of alcohol intake can lead to alcohol dependence, which can cause damage to mental and multiple organ functions, among which the damage to liver function and mental function is the most common and serious. The addiction, abuse and dependence of ethanol is one of the most serious socioeconomic and public health problems in the world today, and alcohol-related liver disease has become an important cause of death in western countries. Therefore, the determination of blood alcohol plays a pivotal role in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/98G01N21/31
Inventor 邹炳德沃燕波
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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