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EB (Epstein-Barr) virus Zta IgA (Immunoglobulin A) antibody colloidal gold detection kit and preparation method thereof

A detection kit and EB virus technology, applied in the field of medicine and biology, can solve the problems of complex operation and long detection time of ELISA method

Active Publication Date: 2014-03-12
中山生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the ELISA method is complicated to operate, and the detection time is long, and operations such as dilution, incubation, separation, washing, and color development of the sample to be tested are required.

Method used

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  • EB (Epstein-Barr) virus Zta IgA (Immunoglobulin A) antibody colloidal gold detection kit and preparation method thereof
  • EB (Epstein-Barr) virus Zta IgA (Immunoglobulin A) antibody colloidal gold detection kit and preparation method thereof
  • EB (Epstein-Barr) virus Zta IgA (Immunoglobulin A) antibody colloidal gold detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] The base plate 1 is a PVC plate, and the reaction membrane 2 is an NC film. Embodiment 1: the preparation of kit

[0110] 1. Reagent preparation

[0111] (1) Preparation of 0.05M PBS buffer solution (according to 100ml volume):

[0112] Accurately weigh Na 2 HPO 4 12H 2 O 1.450g, NaH 2 PO 4 2H 2 O 0.148g, NaCl 0.850g, add 80.00mL of purified water, stir to fully dissolve, then dilute to 100.00mL with purified water, mix well, store at 2~8℃ for later use, valid for 14 days.

[0113] (2) Preparation of 1% chloroauric acid solution (according to 100ml amount):

[0114] Weigh 1g of chloroauric acid, add 80ml of purified water to dissolve it, add purified water to make up to 100ml, mix well, wrap it in aluminum foil, and store it at 2-8°C for later use, with a validity period of 12 months.

[0115] (3) Preparation of 0.1M potassium carbonate solution (according to 20ml volume):

[0116] Measure 16ml of purified water into the reagent bottle, and accurately weigh 0....

Embodiment 2

[0134] Embodiment 2: the selection of reaction time and sample amount, embodiment is the same as result as shown in table 16.

[0135] Table 16:

[0136]

[0137] Result analysis: From the experimental results in Table 16, it can be seen that the amount of sample added will affect the correct reaction result of the test strip. If the amount of sample added is low, there will be missed detection, and when the amount of sample added is high, false positives will easily occur. Based on the above experimental results, considering the stability of the test results and the convenience of operation, the sample volume was selected to be 10 μl of serum, followed by 100 μl of sample diluent, and 15-25 minutes for interpretation.

Embodiment 3

[0138] Embodiment 3: sample detection

[0139] (1) Preparation of sample diluent (according to 100ml volume):

[0140] Weigh 0.06g of NaH 2 PO 4 2H 2 O and 0.58g Na 2 HPO 4 12H 2 O, add 80.0mL of purified water, stir to fully dissolve, then add 0.85g of NaCl, mix well, set the volume to 100mL, measure the pH value to 7.30-7.50, store at 4-30°C for later use, and the validity period is 14 months.

[0141] (2) Collection and storage of testing samples:

[0142] Serum samples were collected intravenously according to routine methods. Samples determined within 5 days can be stored at 4°C. Samples can be stored at 20°C for at least 3 months. Samples should avoid hemolysis or repeated freezing and thawing. Samples that are turbid or precipitated should be clarified by centrifugation or filtration before testing.

[0143] (3) Detection:

[0144] Take out the reagent card from the original packaging aluminum foil bag, place it flat on the horizontal workbench and mark the ...

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Abstract

The invention discloses an EB (Epstein-Barr) virus Zta IgA (Immunoglobulin A) antibody colloidal gold detection kit and a preparation method thereof. The kit comprises a bottom plate, wherein a reaction film provided with a detection line and a quality control line is stuck to the bottom plate; a gold mark mat and a sample mat are stuck to one end of the reaction film which is close to the detection line in sequence in a laminated way; an absorbing mat is stuck to one end of the reaction film which is close to the quality control line in a laminated way; the detection line on the reaction film is coated with a recombinant EB virus Zta antigen; the quality control line is coated with a goat anti-mouse IgG antibody; and the gold mark mat is coated with a mouse anti-human IgA monoclonal antibody marked with colloidal gold. The kit has the advantages of rapid, simple, convenient detection, high accuracy, high sensitivity, no need of auxiliary instrument, direct observation of results with naked eyes, wide application range, single detection, easiness for popularizing and stable product quality in the effective period of the kit. The detection result of the kit is not interfered with a rheumatoid factor, a hepatitis B virus antibody, a treponema pallidum antibody, a human immunodeficiency virus antibody, a cytomegalovirus antibody and a herpes simplex virus antibody positive specimen.

Description

【Technical field】 [0001] The invention relates to an antibody detection kit, in particular to a colloidal gold detection kit for detecting Epstein-Barr virus Zta IgA antibody and a preparation method thereof, belonging to the field of medical biotechnology. 【Background technique】 [0002] Epstein-barr virus (EBV) is a γ-herpes virus that is addicted to human B lymphocytes. It mainly invades B lymphocytes and affects human B lymphocytes, epithelial cells (including parotid duct, pharynx, and cervix) and glandular cells. have affinity. In 1997, the World Health Organization's annual meeting of the International Union Against Cancer classified EBV as a first-class carcinogen. Zta protein is an early activating factor of Epstein-Barr virus expressed by the immediate early gene BZLF1. It is a product of the immediate early phase of latent infection transformed into lytic infection. It plays an important role in the transition to lytic infection, as well as in the transcription ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
Inventor 王胜岚向柱方李峰宋小冬
Owner 中山生物工程有限公司
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