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Device and process for monitoring oceanic planktonic microorganism content in real time and early warning red tide

A technology for microbial biomass and real-time monitoring, applied in analytical materials, instruments, etc., can solve the problems of insufficient comprehensiveness, high cost and slow speed, etc., and achieve the effect of improving the utilization rate of light energy and improving the oxidation capacity.

Inactive Publication Date: 2012-07-04
YANTAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, (1) and (2) two methods are used for biological identification, but the speed is relatively slow when used for content determination; (3) and (4) are mainly used for determining the content of chlorophyll, and the samples need to be concentrated or extracted before the test , so it can only be used for laboratory determination, not for online determination; although (5), (6), and (7) have the possibility of realizing online automatic determination, they are only applicable to special biological species with known structures , so there are still deficiencies in its comprehensiveness; (8) Although online automatic determination can be realized, it is easily affected by weather factors and the cost is relatively high

Method used

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  • Device and process for monitoring oceanic planktonic microorganism content in real time and early warning red tide
  • Device and process for monitoring oceanic planktonic microorganism content in real time and early warning red tide
  • Device and process for monitoring oceanic planktonic microorganism content in real time and early warning red tide

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 Marine planktonic biomass real-time monitoring and red tide early warning device, which mainly includes sequentially connected sampler, acidifier 4, gas-water separator 7, oxidation reactor 9, condensation dehydrator 12, ion trap 13 and infrared CO 2 Detector 14, the gas-water separator 7 is provided with an exhaust port 8, the condensation dehydrator is provided with a drain pipe 21, the oxidation reactor 9 is provided with an ultraviolet lamp 11 and an electric heating jacket 10, and the sampler It mainly includes sampling port 1, reagent bottle 5, and carrier gas bottle 6. Sampling port 1 is connected to acidifier 4 through filter 2, flow sensor 22, peristaltic pump 3, and reagent bottle 5 is connected to acidifier through flow sensor 22, peristaltic pump 3. 4 are connected, and the carrier gas cylinder 6 is divided into two paths through a three-way solenoid valve, one of which is connected with the acidifier 4.

[0033] The inner diameter of the sampli...

Embodiment 2

[0048] Example 2 is a real-time monitoring and red tide warning device for marine planktonic microbial biomass. The difference from Example 1 is that the inner diameter of the sampling tube between the sampling port and the acidifier is 2.0 mm. The mesh size of the filter is 3.0 mm. The acidifying agent in the reagent bottle is sulfuric acid, the oxidizing agent is ammonium persulfate, and the mass ratio of sulfuric acid to ammonium persulfate is 4:1.

experiment example 1

[0049] In Experimental Example 1, Papua viridis, Salina and Chlorella were respectively selected for cultivation. The culture container is a 20L transparent glass container. The cultivation process is as follows: first soak the glass container with 1:5 HCl, then rinse with distilled water and double distilled water, and finally wash and steam sterilize (121°C, 0.56kg·cm -2 , 30min) and dried before use, to keep the sterility of the container, so that the cultured algae grow more beneficial. Select seawater for cultivating algae and filter it through a filter membrane with a pore size of 0.45 μm, then boil and sterilize it in a pressure cooker (121°C, 0·56kg·cm -2, 30min), after standing for 24 hours to balance, the nutrient solution was prepared according to the f / 2 formula to improve the favorable nutritional environment for algae. The experimental algae species were cultivated under light conditions with light intensities of 10000lx and 15000lx (400-700nm) respectively, wi...

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Abstract

The invention aims at providing a device and a process for monitoring oceanic planktonic microorganism content in real time and early warning red tide based on total organic carbon (TOC) automatic analysis. By increasing the size of a sample injection tube and improving oxidation capacity of an oxidation reactor, the TOC including planktonic microorganism in seawater can be automatically and continuously determined on line. According to the corresponding relation of oceanic planktonic microorganism indexes (including number, chlorophyll content, biological turbidity and the like) and the TOC,the oceanic planktonic microorganism content is monitored in real time. According to the variation trend of the oceanic planktonic microorganism content, early warning of red tide disasters is provided. Compared with other analytical methods, the device and the process for monitoring the oceanic planktonic microorganism content in real time and early warning the red tide is simple, convenient andaccurate.

Description

technical field [0001] The invention relates to a device and process for real-time monitoring of marine planktonic microbial mass and red tide early warning. Background technique [0002] More than 70% of the earth's surface is ocean, and more than 40% of the global primary productivity comes from the photosynthesis of marine planktonic microorganisms. As the most important primary producer, marine phytoplankton is located at the first level of the marine biological food chain, so its species composition and quantity will directly affect the total productivity of the water body. However, in some cases, if the marine phytoplankton proliferates suddenly and forms a "red tide", it will bring extremely serious adverse effects on the marine environment and marine ecology. Therefore, mastering the biomass of marine plankton and its changing dynamics is of great significance for the development of marine biological resources. [0003] All marine planktonic microorganisms all cont...

Claims

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Application Information

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IPC IPC(8): G01N35/00
Inventor 徐惠忠徐滋秋周明徐成彬付万年
Owner YANTAI UNIV
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