Hole channel amplification method of porous ceramic microballoon sphere

A technology of porous ceramics and microspheres, which is applied in the field of pore expansion of porous ceramic microspheres, which can solve the problems of large limitations of amplified channels, and achieve the effects of improving mass transfer efficiency, less environmental pollution, and simple preparation processes

Active Publication Date: 2012-07-11
嘉兴千纯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional porous ceramic pore amplification method is mainly used to prepare larger porous materials, and the porous ceramic microspheres used for protein liquid chromatography have a particle size of 30-200 μm, and the traditional method is directly used to amplify the pore More limited

Method used

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  • Hole channel amplification method of porous ceramic microballoon sphere
  • Hole channel amplification method of porous ceramic microballoon sphere
  • Hole channel amplification method of porous ceramic microballoon sphere

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Mix 30g of nano-zirconia, 2g of citric acid, 30mL of water, 5g of nano-carbon powder and 20mL of 5% sodium alginate solution until it becomes a gel. Transfer the above ceramic slurry to a 500mL three-neck flask, add 200mL pump oil, 10mL Span 80, stir at 700r / min for 30min, add 10mL saturated calcium chloride dropwise, and continue stirring for 10min. After the reaction, the microspheres were collected by filtration, cleaned with alcohol and deionized water, dried in an oven at 50°C, heated to 1450°C in a muffle furnace, kept for 20 minutes, and cooled naturally to obtain porous ceramic microspheres. The obtained porous ceramic microspheres have an average pore size of The maximum aperture is The porosity is 60%, and the specific surface area is 22m 2 / cm 3 , with an average particle size of 120 μm.

Embodiment 2

[0022] Mix 28g of nano-titanium dioxide, 1g of citric acid, 30mL of water, 5g of polyethylene glycol and 20mL of 5% sodium alginate solution until gelled. Transfer the above ceramic slurry to a 500mL three-neck flask, add 200mL pump oil, 10mL Span 80, stir at 600r / min for 30min, add 10mL saturated calcium chloride dropwise, and continue stirring for 10min. After the reaction, the microspheres were collected by filtration, cleaned with alcohol and deionized water, dried in an oven at 50°C, heated to 1300°C in a muffle furnace, kept for 20 minutes, and cooled naturally to obtain porous ceramic microspheres. The obtained porous ceramic microspheres have an average pore size of The maximum aperture is The porosity is 62%, and the specific surface area is 22.6m 2 / cm 3 , with an average particle size of 98 μm.

Embodiment 3

[0024] Mix 12g of nano-titanium dioxide, 2g of citric acid, 30mL of water, 4g of polyvinyl alcohol and 20mL of 5% sodium alginate solution until gelled. Transfer the above ceramic slurry to a 500mL three-necked flask, add 200mL pump oil, 10mL Span 80, stir at 800r / min for 40min, add 10mL saturated calcium chloride dropwise, and continue stirring for 10min. After the reaction, the microspheres were collected by filtration, cleaned with alcohol and deionized water, dried in an oven at 60°C, heated to 1000°C in a muffle furnace, kept for 20 minutes, and cooled naturally to obtain porous ceramic microspheres. The obtained porous ceramic microspheres have an average pore size of The maximum aperture is The porosity is 58%, and the specific surface area is 20.7m 2 / cm 3 , with an average particle size of 118 μm.

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Abstract

The invention discloses a hole channel amplification method of a porous ceramic microballoon sphere for liquid chromatography, which enables silica, zirconium dioxide or titanium dioxide powder to serve as ceramic framework materials, enables nanometer powdered carbon, ammonium chloride, polyving akohol, urea, polyethylene glycol, sodium chloride, sodium sulfate, sodium phosphate, potassium phosphate, potassium chloride, potassium sulfate and the like to serve as porogen, and prepares macropore ceramic microballoon spheres through reversed phase suspension and high temperature sintering. The specific steps are as following: (1) preparing ceramic slurry, enabling the ceramic framework materials, the porogen and sodium alginate solution to be mixed; (2) preparing the porous ceramic microballoon sphere, enabling the ceramic slurry to be conducted reversed phase suspension and dispersion in an oil phase, adding saturation calcium chloride solution in a dropping mode, solidifying to from the sphere, and molding by high temperature sintering; and (3) removing residual porogen, enabling the ceramic microballoon sphere to be soaked in water, hydrochloric acid or sodium hydroxide solution, shaking to dissolve the residual porogen. The microballoon sphere developed by the method has the advantages of having rigidity, and being big in hole diameter and good in spherical degree, and the microballoon sphere can be used for substrates of large biological molecule chromatographic separation media.

Description

technical field [0001] The invention relates to a channel amplification method of porous ceramic microspheres Background technique [0002] The development of biotechnology has provided the possibility for the large-scale production of protein drugs with high added value. The industrialized large-scale purification of protein drugs, higher purity standards and economic cost requirements have promoted the great development of bioseparation technology. Liquid chromatography is an important unit in the bioseparation process and plays an important role in the biopharmaceutical industry. Since Peterson and Sobers invented cellulose-based ion-exchange chromatography media in the mid-1950s and successfully applied them to protein purification, chromatography media have played a major role in large-scale operation, resolution, separation efficiency, selectivity, and adsorption capacity. The performance has been continuously improved. However, the development of chromatography med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B38/00
Inventor 夏海锋吴璞强金雄华郑梦杰
Owner 嘉兴千纯生物科技有限公司
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