Method for extracting purified complanatuside from milk vetch seed
A technology of sarcoside and sarcoside, which is applied in the field of natural medicinal chemistry, can solve the problems of large solvent residues, etc., and achieve the effects of low production cost, high product purity and short production cycle
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Embodiment 1
[0018] Take 5 kg of Astragalus complanatus raw material and put it into a small extraction tank, add 8 times the amount of 2% sodium hydroxide solution to percolate and extract until the percolate color becomes lighter, combine the percolate, and add a tubular composite ultrafiltration membrane system with a molecular weight cut-off of 6000. Filter, concentrate the permeate into an extract, add hydrochloric acid to adjust the pH to 3.5, filter the precipitate, heat the precipitate to reflux and dissolve, let it cool, extract twice with 5 times the volume of petroleum ether, add 2.2% CTS Chinese medicine clarifier to the water layer, Leave it to stand, centrifuge, and put the clear liquid on the HZ818 macroporous adsorption resin column, first wash away impurities with water, then eluted with 50% ethanol, collect the eluate, and concentrate it into an extract. Suspend 4 kg of 100-200 mesh silica gel with ethyl acetate and add it to a stainless steel chromatography column with a c...
Embodiment 2
[0020] Take 5kg of Astragalus complanatus raw material and put it into a small extraction tank, add 15 times the amount of 3% lime water solution and ultrasonically extract 4 times, 1 hour each time, combine the extracts, add a tubular composite ultrafiltration membrane system with a molecular weight cutoff of 4000 to ultrafiltration. After the liquid is concentrated into an extract, add hydrochloric acid to adjust the pH to 3.0, filter out the precipitate, heat the precipitate to dissolve under reflux, let it cool, extract 3 times with 4 times the volume of petroleum ether, add 1.5% deacetyl chitosan to the water layer, and let it stand. After centrifugation, the clear liquid was applied to the ADS-21 macroporous adsorption resin column, first washed with water to remove impurities, and then eluted with 70% ethanol, the eluate was collected and concentrated into an extract. Suspend 6 kg of 200-280 mesh silica gel with ethyl acetate and add it to a stainless steel chromatography...
Embodiment 3
[0022] Take 10kg of Astragalus complanatus raw material and put it into a small extraction tank, add 20 times the amount of 5% ammonia water alcohol solution (alcohol concentration is 50%), decoct, filter, combine the extracts, and add a tubular composite ultrafiltration membrane system with a molecular weight cut-off of 5000. After the permeate is concentrated into an extract, add hydrochloric acid to adjust the pH to 4.0, filter out the precipitate, heat the precipitate to dissolve under reflux, let it cool, extract twice with 8 times the volume of petroleum ether, add 1.2% CTS Chinese medicine clarifier to the water layer, and statically After setting, centrifuging, the clear liquid is applied to the LS-303 macroporous adsorption resin column, the impurities are first washed with water, and then eluted with 40% ethanol, the eluate is collected, and concentrated into an extract. After suspending 10 kg of 150-250 mesh silica gel with ethyl acetate, evenly add it to a stainless ...
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