System for treating sewage and producing biological oil by using microalgae and method

A bio-oil and sewage technology, applied in energy wastewater treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of limited photobioreactor development, achieve an excellent algae cell growth environment, convenient operation, The effect of uniform distribution of bubbles

Active Publication Date: 2012-07-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although microalgae have high environmental and economic value, the real realization of industrialized economic utilization of microalgae is still limited by the development of photobioreactors for the industrial scale cultivation of microalgae

Method used

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  • System for treating sewage and producing biological oil by using microalgae and method
  • System for treating sewage and producing biological oil by using microalgae and method
  • System for treating sewage and producing biological oil by using microalgae and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] The common f / 2 medium (pH 7.0) for green algae was used for seed liquid culture in shake flasks, and sewage medium (pH 7.0) was used for seed tank culture and photobioreactor 1 culture. The sewage medium is the secondary effluent treated by the sewage treatment plant after the municipal sewage and industrial sewage are filtered out to remove bacteria or algae (total nitrogen is 30.0-50.0mg / L, ammonia nitrogen is 10.0-16.0mg / L, total phosphorus Concentration of 2.0 ~ 4.0mg / L) formed medium, through the feed port / inoculation port 19 into the photobioreactor 1 and sterilized, the initial inoculation density of 6.0 × 10 7 cfu / ml, stirring speed is 120rpm, light intensity is 15000lux, light cycle is 16h:8h, culture temperature is 30℃, pH is 7.0, CO 2 gas CO 2 The concentration is 30% (v / v), the ventilation rate is 0.1VVm, and the algal concentration in the photobioreactor 1 is as high as 2.0×10 before split culture and after 48 hours of culture. 8 cfu / ml, and the content...

Embodiment 2

[0084]Using continuous fermentation and using municipal sewage and industrial sewage after filtering out miscellaneous bacteria or miscellaneous algae, the secondary effluent treated by the sewage treatment plant as the medium, inoculate the medium with Chlorella C9-JN2010, wherein, the initial The connection density is 3.5×10 7 cfu / ml, the light cycle is 14h:10h, the light intensity is 5000lux, the culture temperature is 25°C, the pH is 8.5, CO 2 gas CO 2 Concentration is 25.0% (v / v), ventilation rate is 0.2VVm, continuous culture 480h, nitrogen, phosphorus removal effect, CO 2 The conversion rate (% (v / v)) and oil accumulation relationship see Figures 7A-7B shown.

[0085] Depend on Figure 7A The analysis shows that in the early stage of continuous culture, as the culture time prolongs, its response to nitrogen, phosphorus and CO 2 utilization is accelerated. From 0 to 120 hours, the utilization rate of nitrogen increased continuously, and then the removal rate reach...

Embodiment 3

[0088] The municipal sewage and industrial sewage after filtering out miscellaneous bacteria or miscellaneous algae by adopting batch fermentation method are used as the culture medium through the secondary effluent of the sewage treatment plant, and the culture medium is inoculated with Chlorella C9-JN2010, wherein, the initial inoculation Density is 1.0×10 7 cfu / ml, the light cycle is 10h:14h, the light intensity is 7500lux, the culture temperature is 20°C, the pH is 6.5, CO 2 The concentration is 15.0% (v / v), the ventilation rate is 0.01VVm, and the batch culture is 480h, the removal effect of nitrogen and phosphorus, CO 2 The conversion rate (% (v / v)) and oil accumulation relationship see Figures 8A-8B shown.

[0089] Depend on Figure 8A The analysis shows that in the early stage of culture, with the extension of culture time, its response to nitrogen, phosphorus and CO 2 utilization is accelerated. The utilization rate of nitrogen continued to increase within 0-168...

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Abstract

The invention provides a system for treating sewage and producing biological oil by using microalgae. A first light source device and a second light source device of the system are respectively arranged in a photo-biological reactor and an oil accumulating tank; a CO2 gas supply device is respectively connected with the photo-biological reactor and the oil accumulating tank through air passages; a sewage sedimentation tank is connected with the oil accumulating tank through a pipeline and connected with the photo-biological reactor via a pipeline through a microalgal liquid mixing tank; the photo-biological reactor is respectively connected with the microalgal liquid mixing tank and the oil accumulating tank via pipelines through a first microalgal liquid separating device; and the oil accumulating tank is connected with a second separating device through a pipeline. The invention further provides a method for using the system to treat sewage and produce biological oil. Through the use of the invention, the production of biological energy sources and other byproducts (such as protein, cosmetic raw materials, animal feed and the like) is realized while sewage is treated, so that the production cost is lowered, and the comprehensive utilization of waste resources and the energy-saving and emission-reducing green production are achieved. The invention is suitable for cultivating microalgae from a laboratory to a factory, is skillful and unique in design, and is applicable to large-scale promotion and application.

Description

technical field [0001] The present invention relates to the technical field of environmental engineering and biological energy, more specifically, to the technical field of photobioreactor, in particular to a system and method for using microalgae to treat sewage and produce bio-oil. Background technique [0002] Today, energy shortage, environmental pollution and food shortage are three major world problems. Microalgae play a very important role in solving the above problems. [0003] Microalgae are photoautotrophic microorganisms with high light utilization efficiency and fast and efficient uptake of nutrients from the environment (such as CO 2 , nitrogen, phosphorus, etc.) and the ability to transform into organic compounds, is the primary producer in the ecosystem, plays a huge role in the entire material cycle, has high production efficiency, and has attractive development prospects. Microalgae culture only needs some simple nutrients, ammonium or nitrate, phosphate a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12P7/64
CPCY02W10/37
Inventor 杨海麟张玲夏小乐辛瑜王武李昌灵李宇佶
Owner JIANGNAN UNIV
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