Preparation method of lactic acid by saccharifying and fermenting lignocellulose

A technology of lignocellulose and cellulase, which is applied in the direction of fermentation, can solve the problems of less research and application of lactic acid, low lactic acid concentration, and high production cost, and achieve low substrate cost, high lactic acid concentration and yield, and low cost Effect

Inactive Publication Date: 2012-07-18
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there are many technical difficulties in the fermentation of lignocellulose as the substrate, there are few related studies and applications on the production of lactic acid by fermentation. Even if there are reports, there are still problems such as low lactic acid concentration, many added nutrients, and high production costs.

Method used

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  • Preparation method of lactic acid by saccharifying and fermenting lignocellulose
  • Preparation method of lactic acid by saccharifying and fermenting lignocellulose
  • Preparation method of lactic acid by saccharifying and fermenting lignocellulose

Examples

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Effect test

Embodiment 1

[0039] Dried corn stalks were mixed with dilute sulfuric acid solution until the water content was 33%, the sulfuric acid concentration was 2.5%, and pretreated at 190°C for 3min; Solid state culture for 7 days; Harvest the material without high-temperature sterilization, add it to the fermenter, the solid content in the fermenter is 30% by mass, the amount of cellulase is 15 FPU / g DM, pre-saccharification at 50°C for 10 hours, 5M hydrogen Sodium oxide controlled the pH of the fermentation process at 5.5, and fermented at 42°C for 48 hours. Because the process adds a large amount of lye, the final volume calculation solid content is 26.7%.

[0040] The Pediococcus acidilactici used in the examples is Pediococcus acidilactici DQ2 isolated and preserved from lignocellulose.

[0041] Pediococcus acidilactici DQ2 is isolated from lignocellulose hydrolyzate, spread the hydrolyzate sample containing microorganisms on YPD medium agar plate, culture at 42°C, and test whether the grow...

Embodiment 2

[0043] Dried corn stalks are mixed with dilute sulfuric acid solution until the water content is 33%, the sulfuric acid concentration is 2.5%, and pretreated at 190°C for 3 minutes. After the pretreatment, the material is adjusted to pH 5.4, and the water content is 58%. Solid state culture for 4 days; Harvest the material, store the material in a refrigerator at 4°C for half a month, the material is not sterilized, add 30% solid mass fraction to the fermenter, add enzyme amount 13FPU / g DM, use 5M sodium hydroxide solution to control pH 5.0, 42 ℃ fermentation.

Embodiment 3

[0045]The dried corn stalks were mixed with dilute sulfuric acid solution until the water content was 33%, the sulfuric acid concentration was 2.5%, and pretreated at 190°C for 3 minutes. After the pretreatment, the material was adjusted to pH 5.4 and the water content was 58%. Add cellulase 15FPU / gDM to the fermenter with a solid mass fraction of 30%, 50°C, pH 5.0, hydrolyze for 48H, centrifuge at 13000rpm for 10min to obtain a supernatant; insert the cultured supernatant into the supernatant containing high sugar concentration Pediococcus lactis seeds were fermented at 42°C, pH 5.0.

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Abstract

The invention discloses a preparation method of lactic acid, which takes lignocellulose as raw material, and is low in cost and high in efficiency, wherein the preparation method specifically comprises the following steps of: separating from lignocellulose to store pediococcus acidilactici, carrying out liquid culture on the pediococcus acidilactici to obtain cell bacteria taken as seeds, inoculating into a reactor with high lignocellulose content, adding cellulase, and synchronously carrying out saccharification and high-temperature fermentation to generate the lactic acid; or firstly saccharifying the cellulase of the lignocellulose to obtain sugar-containing hydrolysate, then carrying out high-temperature lactic acid fermentation by the hydrolysate to obtain the lactic acid; or uniformly blending the pediococcus acidilactici and the lignocellulose raw material, carrying out solid state cultivation, adding the pediococcus acidilactici-containing lignocellulose raw material into a fermentation tank, adding the cellulase, and synchronously carrying out saccharification and high-temperature fermentation to prepare the lactic acid. By adopting unique fermentation technology and microorganism, the preparation method is high in product concentration, simple in process, and low in cost; and particularly by taking the lignocellulose as raw material, the preparation method is wide in production space and development prospect.

Description

technical field [0001] The invention relates to the fields of biomanufacturing and biorefining of bio-based chemicals, in particular to a method for producing lactic acid with high concentration and high optical purity through saccharification and fermentation of lignocellulosic raw materials such as corn stalks. Background technique [0002] Lactic acid is widely used in food, medicine, beverage and chemical industries, especially the polylactic acid material prepared by polymerizing lactic acid as a monomer, has excellent performance, can replace plastic, and is a completely degradable biological material. Polylactic acid products are a typical example in the field of biorefinery, and are the first biomaterials that are completely degradable and enter the market, such as the US NatureWorks polylactic acid material. With the reduction of petrochemical resources and the pursuit of an environmentally friendly society, lactic acid, as a monomer capable of producing degradable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56
Inventor 鲍杰楚德强赵凯张建董宏伟
Owner EAST CHINA UNIV OF SCI & TECH
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