Agent for inhibiting thickening of peritoneal membrane
A peritoneal hypertrophy and inhibitor technology, applied in the field of peritoneal hypertrophy inhibitor, can solve problems such as abnormal blood vessels, achieve the effects of strong lipid affinity, reduced drug side effects, and easy operation and use
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0106]
[0107] Intraperitoneal delivery of chlorhexidine in mice (C57 / BL6, 7 weeks postnatal male, weighing 21±1 g) produced a model of peritoneal hypertrophy (Junor BJR, Briggs JD, Forwell MA, Dobbie JW, Henderson I: Sclerosing peritonitis - The cotribution of chlorhexidine in alcohol. Perit Dial Bull 101-104, 1985). Specifically, the mice were divided into three groups: CG group (5 rats), control group (4 rats), and TMG group (5 rats). Do the following for each group.
[0108] (CG group)
[0109] 0.2 mL of a solution of 0.1% chlorhexidine / 15% ethanol / physiological saline was intraperitoneally delivered to the mice three times a week.
[0110] (control group)
[0111] 0.2 mL of a 15% ethanol / physiological saline solution was intraperitoneally delivered to the mice three times a week.
[0112] (TMG group)
[0113] In a solution of 0.1% chlorhexidine / 15% ethanol / physiological saline, add TMG (=2-(α-D-glucopyranosyl)methyl-2,5,7,8- Tetramethylchroman-6-ol for short) int...
Embodiment 2
[0119]
[0120] The result after H.E staining is carried out to the peritoneal tissue section that makes in embodiment 1, as Figure 1-A , Figure 1-B , Figure 1-C shown. In addition, the thickness of the peritoneum was measured, and each mouse was randomly measured at 10 locations, and then the average value was calculated. The results are shown in Table 1.
[0121] [Table 1]
[0122]
Peritoneal thickness (μm)
[0123] CG group
82.3±24.5
refer to Figure 1-A
TMG group
42.1±19.7 a
refer to Figure 1-B
control group
9.1±2.8 b
refer to Figure 1-C
[0124] a Pb P<0.0001 versus CG(t test)
[0125] from Figure 1-A~Figure 1-C From the results in Table 1, it became clear that the peritoneal hypertrophy phenomenon caused by CG delivery was effectively suppressed in the TMG delivery group.
Embodiment 3
[0127]
[0128] The peritoneal tissue sections prepared in Example 1 were immunostained using the polyclonal antibody RABBIT ANTI MOUSE COLLAGEN I (polyclonal IgG) (AbD Serotec; UK) against type I collagen, and the results were as follows: Figure 2-A , Figure 2-B , Figure 2-C shown. Antibody activation was performed in an autoclave, and immunostaining was performed using antibodies diluted 250-fold. Under the condition of 400 times magnification of the field of view, the proportion of type I collagen targeting the peritoneum on the wall side of each mouse was measured, and 10 parts were randomly measured during the measurement, and then the average value was calculated. The results are shown in Table 2.
[0129] [Table 2]
[0130]
Type I collagen occupied area (%)
CG group
18.55±2.06
refer to Figure 2-A
TMG group
8.68±1.85 a
refer to Figure 2-B
control group
1.94±0.46 b
refer to Figure 2-C
...
PUM
| Property | Measurement | Unit |
|---|---|---|
| thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More