Method for rapidly and quantitatively detecting organophosphorus pesticide residues in soil

A technology for the quantitative detection of organophosphorus pesticides, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. It can solve the problems of repeatability, recovery rate, poor sensitivity, etc. , to achieve the effect of fast detection speed, high sensitivity and easy operation

Active Publication Date: 2012-08-08
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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AI Technical Summary

Problems solved by technology

The biggest advantage of the enzyme inhibition method is that it is easy to operate, fast, and does not require expensive instruments. It is especially suitable for on-site detection and screening of a large number of samples, and is easy to popularize; but the sensitivity is lower than that of the instrument method, and the repeatability and recovery rate need to be improved.
[0004] According to the data, at present, the rapid detection method of organophosphorus pesticides is mainly the enzyme inhibition method, and this method is mainly used for the qualitative or semi-quantitative detection of organophosphorus pesticide residues in various vegetables, fruits and foods, while for organic phosphorus pesticides in soil There are few methods for the rapid quantitative detection of phosphorus pesticide residues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Sample treatment: After fully grinding the air-dried soil sample containing methyl parathion and the blank soil sample, pass through a 100-mesh sieve, accurately weigh 1.00g of the soil sample, add 5mL of pH8.0 phosphate buffer and mix on the mixer for 3- Mix well for 5 minutes, place at room temperature for 10 minutes, centrifuge at 6000r / min for 10 minutes, and take the supernatant for later use.

[0039] Test of blank control soil sample: take a clean test tube, then add 2.5mL blank soil sample supernatant to the test tube, then add 0.1mL enzyme solution, 0.1mL chromogen, shake well and place at 37°C for 15min Above (the action time of each batch of samples should be consistent). Use a spectrophotometer to measure the absorbance value A before the reaction at 412nm 0 , add 0.1mL substrate and shake well, then detect it immediately, record the absorbance value after the reaction, and calculate the change amount ΔA of the absorbance value before and after the reaction...

Embodiment 2

[0047] Sample processing and the test method of blank control soil sample are with embodiment 1;

[0048] Preparation of standard curve: Take another 5 clean test tubes, add 0.1mL of different concentrations of phorate standard solution and 2.4mL of blank soil sample supernatant to them respectively, so that the final concentrations are 0.01, 0.1, 1, 10, 100mg / mL, then add 0.1mL enzyme solution and 0.1mL chromogenic agent respectively, and the rest of the operation is the same as in Example 1, calculate the change amount ΔA of the absorbance value before and after the reaction 1 , this test requires three repetitions at the same time, to find the amount of change ΔA 1 The average value of 0.01, 0.1, 1, 10, 100 mg / mL of different concentrations of phorate solution to the enzyme inhibitory rate, and then respectively to the different concentrations of pesticide standards log 10 is the abscissa, take the log with the different concentrations of the phorate standard 10 The obtai...

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Abstract

The invention provides a method for rapidly and quantitatively detecting organophosphorus pesticide residues in soil. According to the method, by utilizing the principle that an organophosphorus pesticide has an inhibiting effect on the normal function of cholinesterase under certain conditions and the inhibition rate is in positive correlation with the concentration of the organophosphorus pesticide, under normal conditions, cholinesterase catalyzes a nerve-conduction metabolic product (acetylcholine or butyrycholine) to hydrolyze, the hydrolysis product reacts with a developer so as to generate a yellow substance, a spectrophotometer is utilized to measure the variable quantity of absorbance before and after reaction at a place of 412nm, the inhibition rate is calculated, and whether the high-dose organophosphorus pesticide exists in a sample or not can be judged through the inhibition rate. The method has the advantages of simplicity and convenience in operation, high detection speed, no need of expensive instruments, high sensitivity, good repetitiveness, high recovery rate and the like.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for rapid and quantitative detection of organophosphorus pesticide residues in soil. Background technique [0002] Extensive use of organophosphorus pesticides has caused serious environmental pollution and endangered human health. The development of rapid detection methods for organophosphorus pesticides has attracted more and more attention. At present, the analytical methods for detecting organophosphorus pesticides mainly include spectroscopy, chromatography and enzyme inhibition. The sensitivity of the spectrum method is not high, and generally it can only be used as a qualitative identification method for rough selection; chromatography is currently the most important detection method for the detection of organophosphorus pesticides, and can be divided into thin-layer chromatography, There are three types of gas chromatography and high performance liquid ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 王继雯慕兰杨文玲甄静谢宝恩刘莹莹李冠杰周伏忠陈国参
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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