Supercharge Your Innovation With Domain-Expert AI Agents!

Hainanmycin fermentation method

A fermentation method and the technology of Hainanmycin, applied in the field of biomedicine, can solve the problems of difficulty in maintaining the stability of strains and the inability of fermentation units to meet production requirements, and achieve the effects of optimizing composition and inoculum amount, stabilizing fermentation results and increasing yield.

Active Publication Date: 2012-08-15
山东胜利生物工程有限公司
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the spores need to be cultured for 2-3 generations on a slant, it is difficult to maintain the stability of the strain, and the fermentation unit cannot meet the production requirements

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Incline culture of spores

[0032] Under sterile conditions, use an inoculation spoon to take out a spoonful of sand from the sand tube and spread it evenly on the slope of the blank eggplant bottle, then spread it evenly, plug the mouth of the bottle tightly with a cotton plug, tie it with gauze, and incubate at 28°C for 7 days;

[0033] The slant medium used is: cornstarch 1.0wt%, yeast powder 1.0wt%, calcium carbonate 0.8wt%, sodium chloride 0.3wt%, soybean cake powder 0.5wt%, glucose 1.0wt%, peptone 0.1wt%, magnesium sulfate 0.05wt%, dipotassium hydrogen phosphate 0.02wt%, agar 2.0wt%, the rest of drinking water.

[0034] 2. Preparation of spore suspension

[0035] Use 50ml of sterile water to wash the slant spores obtained by washing, then add an equal volume of sterile 20wt% glycerol, mix well, and make a spore suspension, which contains about 10 per milliliter of suspension. 9 spores, the suspension was divided into 5ml low-temperature-resistant plastic tube...

Embodiment 2

[0042] 1. Incline culture of spores

[0043] Under sterile conditions, use an inoculation spoon to take out a spoonful of sand from the sand tube and spread it evenly on the slope of the blank eggplant bottle, then spread it evenly, plug the mouth of the bottle tightly with a cotton plug, tie it with gauze, and incubate at 28°C for 7 days;

[0044] The slant medium used is: cornstarch 1.0wt%, yeast powder 1.0wt%, calcium carbonate 0.8wt%, sodium chloride 0.3wt%, soybean cake powder 0.5wt%, glucose 1.0wt%, peptone 0.1wt%, magnesium sulfate 0.05wt%, dipotassium hydrogen phosphate 0.02wt%, agar 2.0wt%, the rest of drinking water.

[0045] 2. Preparation of spore suspension

[0046] Use 50ml of sterile water to wash the slant spores obtained by washing, then add an equal volume of sterile 20wt% glycerol, mix well, and make a spore suspension, which contains about 10 per milliliter of suspension. 9 spores, the suspension was divided into 5ml low-temperature-resistant plastic tube...

Embodiment 3

[0053] 1. Incline culture of spores

[0054] Under sterile conditions, use an inoculation spoon to take out a spoonful of sand from the sand tube and spread it evenly on the slope of the blank eggplant bottle, then spread it evenly, plug the mouth of the bottle tightly with a cotton plug, tie it with gauze, and incubate at 28°C for 7 days;

[0055] The slant medium used is: cornstarch 1.0wt%, yeast powder 1.0wt%, calcium carbonate 0.8wt%, sodium chloride 0.3wt%, soybean cake powder 0.5wt%, glucose 1.0wt%, peptone 0.1wt%, magnesium sulfate 0.05wt%, dipotassium hydrogen phosphate 0.02wt%, agar 2.0wt%, the rest of drinking water.

[0056] 2. Preparation of spore suspension

[0057] Use 50ml of sterile water to wash the slant spores obtained by washing, then add an equal volume of sterile 20wt% glycerol, mix well, and make a spore suspension, which contains about 10 per milliliter of suspension. 9- 10 10 spores, the suspension was divided into 5ml low-temperature-resistant plas...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Hainanmycin fermentation method, comprising the following steps: inoculating frozen Streptomyces padanus Var dangfangeusls SDSL-HN1109 sand-soil spores on a slant culture medium, culturing at 28 DEG C for 7 days; washing off the spores from the slant culture medium, adding the glycerol to prepare a spore suspension, keeping at a temperature between -70 DEG C and -80 DEGC for standby; inoculating 0.2 ml of the spore suspension in the 45-60ml of a seed culture medium, culturing at 28 DEG C for 40-42 hours to obtain a seed liquid; inoculating the seed liquid in a fermentation culture medium according to a inoculum size being 10-20%, culturing at 28 DEG C for 90-144 hours to obtain a fermentation liquid. The Hainanmycin is prepared from the high-yield Streptomyces padanus Var dangfangeusls, the fermentation result is stable, the repeatability is good and the yield is high. Moreover, in preparation of the fermentation seed, slant culture only needs to be performed once, the purity of the strain is ensured, the strain pollution is avoided and the operation is simplified.

Description

technical field [0001] The invention relates to a method for fermenting antibiotics, in particular to a method for fermenting hainanmycin by using microbial strains, and belongs to the technical field of biomedicine. Background technique [0002] Hainanmycin is white or off-white powder, odorless and tasteless, its English name is Hainanmycin, and its molecular formula is C 47 h 80 o 15 h 2 O, molecular weight 884. Hainanmycin is a monovalent ionophore antibiotic veterinary drug independently developed in China. It has a significant effect on preventing chicken coccidiosis infection and improving the feed conversion rate of ruminants. It is a highly effective anticoccidial drug. It has a broad antibacterial spectrum against Gram-positive bacteria such as bacilli and cocci, and also has antibacterial effects against fungi such as anaerobic and certain plant pathogens, rice sheath blight and rape sclerotinia; it has no activity against Gram-negative bacteria . Nanhaimyci...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P17/16C12R1/465
Inventor 马景武赵硕珍王炜张旭刘宁李承钰
Owner 山东胜利生物工程有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com