Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus plantarum and application thereof in fermenting and ensiling sweet potato stem and leaf

A Lactobacillus plantarum, stem and leaf technology, applied in the directions of application, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of rapid loss of nutrients, pollution, waste, etc., and achieve the effect of low cost and high efficiency

Inactive Publication Date: 2012-08-22
ZHENGZHOU UNIV
View PDF4 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The stems and leaves of sweet potato (IPomoea batatas) are rich in amino acids, crude fiber, vitamins and some trace elements, etc. They can be eaten, and they are also a good source of feed for livestock and poultry. However, during the harvest period, there are large quantities, easy dehydration, and rapid loss of nutrients. , very difficult to store
Sweet potato stems and leaves are rich in resources in our country, but except for a small part that is used, most of them are discarded and dumped directly into the environment in the form of accumulation, causing great pollution and waste

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus plantarum and application thereof in fermenting and ensiling sweet potato stem and leaf
  • Lactobacillus plantarum and application thereof in fermenting and ensiling sweet potato stem and leaf
  • Lactobacillus plantarum and application thereof in fermenting and ensiling sweet potato stem and leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. Isolation, purification and identification of Lactobacillus plantarum Z3-1CGMCC No.5027

[0044] Take 10g of silage corn stalks in Zhengzhou, Henan, add 90mL of distilled water, shake with a shaker for 5min, 10 -1 -10 -5Gradient dilution. Take 20 μL from each gradient dilution solution and spread it on the MRS solid medium, let it stand for anaerobic culture for 48 hours, pick a single colony with obvious differences in colony shape, size, color and gloss, and repeat the streaking until the obtained Pure colonies. The strains were inoculated in MRS liquid medium and cultured at 30°C for 24 h, centrifuged at 10,000 rpm for 10 min, and 20 μL of the supernatant was taken to inoculate Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis ), Salmonella enterica, Pseudomonas aeruginosa and Escherichia coli were respectively used as indicator bacteria, and the bacteriostasis test was carried out by the Oxford cup double plate method, as figure 1 As shown,...

Embodiment 2

[0051] Embodiment 2, the growth of Lactobacillus plantarum (Lactobacillus plantarum) Z3-1CGMCC No.5027 under different pH, temperature and salt concentration environmental conditions

[0052] Adjust the initial pH of the MRS medium to 2.0, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 7.0, 8.0, 9.0, and 30°C with 1 mol / L hydrochloric acid and 1 mol / L sodium hydroxide, respectively Cultivate Lactobacillus plantarum (Lactobacillus plantarum) Z3-1CGMCC No.502724h at a constant temperature, without adjusting the acid during the cultivation process, and measure the light absorption value at 600nm with a spectrophotometer (OD 600 ). The results are shown in Table 2, the Z3-1 strain can grow under the conditions of initial pH2.5-pH9.0.

[0053] Inoculate the activated Lactobacillus plantarum (Lactobacillus plantarum) Z3-1CGMCC No.5027 strain into MRS liquid medium and culture them at 5, 10, 15, 20, 25, 30, 35, 40, 42, 50°C and other temperatures 24h, the light absorption value (OD) at 600nm w...

Embodiment 3

[0058] Embodiment 3, plant Lactobacillus (Lactobacillus plantarum) Z3-1CGMCC No.5027 fermentation sweet potato stem and leaf

[0059] Preparation of bacterial suspension: under sterile conditions, take Lactobacillus plantarum (Lactobacillus plantarum) Z3-1CGMCCNo.5027, inoculate it in MRS liquid medium and cultivate it at 30°C to obtain a Z3-1 content of 10 8 ~10 9 cfu / mL bacterial suspension.

[0060] Method I

[0061] 1. Set up 2 treatments (each treatment is repeated 3 times, and the results are expressed as mean values):

[0062] Treatment 1 (inoculated with Z3-1 for fermentation): Take 200g sweet potato stems and leaves, 50g water and 2.5gNaCl and mix them, then add 10 per gram of sweet potato stems and leaves 5 The ratio of cfu Z3-1 was added to the above-mentioned Z3-1 bacterial suspension, the obtained mixture was put into a silage bag and mixed evenly, and after the air was pumped (the air pressure in the bag was zero), it was placed anaerobically at 25°C for 3 day...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a lactobacillus plantarum and application thereof in fermenting and ensiling sweet potato stem and leaf. The strain is the Lactobacillus plantarum Z3-1, and has the accession number of CGMCC (China General Microbiological Culture Collection Center) No.5027 in the China General Microbiological Culture Collection Center. The strain can improve a nutrition value after the sweet potato stem and leaf are fermented, and compared with the natural fermentation, the sweet potato stem and leaf are subjected to strain fermentation for 3 days-7days, so that amino acid total quantity, crude protein and soluble total sugar content of the sweet potato stem and leaf are high, and crude fiber content is low. The sweet potato stem and leaf with the bran or the corn grits are mixed to prepare a mixture with the water content of 60%-70% to inoculate the strain for ensiling for 30 days-60days, and then unsealing is carried out, thus pH is low, and lactic acid content is high. The lactobacillus plantarum with the accession number of CGMCC No.5027 provided by the invention can be used for fermenting or ensiling the sweet potato stem and leaf and has the advantages that efficiency is high, cost is low and the like. The lactobacillus plantarum can be used for producing fermented food or green environment-friendly biological feed.

Description

technical field [0001] The invention relates to a plant lactobacillus and its application in sweet potato stem and leaf fermentation and silage. Background technique [0002] The stems and leaves of sweet potato (IPomoea batatas) are rich in amino acids, crude fiber, vitamins and some trace elements, etc. They can be eaten, and they are also a good source of feed for livestock and poultry. However, during the harvest period, there are large quantities, easy dehydration, and rapid loss of nutrients. , very difficult to store. Sweet potato stems and leaves are rich in resources in our country, but except for a small part that is used, most of them are discarded and dumped directly into the environment in the form of accumulation, causing great pollution and waste. Contents of the invention [0003] One object of the present invention is to provide a strain of Lactobacillus plantarum, which is Lactobacillus plantarum (Lactobacillus plantarum) Z3-1, whose registration number ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A23L1/212A23K1/14C12R1/25A23L19/00
Inventor 王雁萍倪奎奎李东霞庞会利谈重芳李宗伟焦浈
Owner ZHENGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com