High-molecular fluorescent microsphere with controllable emission wavelength and preparation method thereof

A technology of emission wavelength and fluorescent microspheres, which is applied in the field of preparation of fluorescent sensing materials, can solve problems such as difficult processing and molding, poor device stability, etc., and achieve the effects of stable properties, guaranteed stability, and good solubility

Inactive Publication Date: 2012-09-12
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

PPV containing substituents has the advantages of good solubility in the subsequent device processing process and is conducive to processing and molding, but it also has the disadvantage of poor device stability; although unsubstituted PPV is stable and easy to obtain, there are Difficult to process and shape

Method used

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  • High-molecular fluorescent microsphere with controllable emission wavelength and preparation method thereof
  • High-molecular fluorescent microsphere with controllable emission wavelength and preparation method thereof
  • High-molecular fluorescent microsphere with controllable emission wavelength and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0028] 1. The PPV precursor solution was prepared by using the salt precursor method: 0.6895 g (1.965 mMol) of polymerized monomer without side chains was dissolved in 12 mL of H 2 O, then slowly add 1.7 mL NaOH aqueous solution into it, stir in an ice-water bath for 1 hour, add 3 mL of 2 M HCl solution to terminate the reaction, and then dialyze with a 3500 DA pore size dialysis bag for 1 day (2×500 mL deionized water H 2 (0) to obtain 20 mL of PPV precursor solution;

[0029] 2. Mix 4 mL of the precursor solution obtained in step 1 with 28 mL of methanol solution. The reaction is carried out under anhydrous and oxygen-free conditions at a reaction temperature of 50°C. After 22 hours of reaction, the methanol is completely removed under reduced pressure to obtain about 3.5mL poly(p-phenylene vinylene) precursor solution containing methoxy group;

[0030] 3. Mix 3 mL of the solution described in step 2 with 0.3 g of sulfonated polystyrene microspheres, shake for 1 hour, cent...

Embodiment 2

[0035] 1. Mix 3 mL of poly(p-phenylene) precursor solution with 0.3 g of sulfonated polystyrene microspheres, shake for 1 hour, centrifuge, remove the supernatant, add 8 mL of deionized water to the microspheres and shake for 1 hour , centrifuge and remove the supernatant. Then use the same volume of deionized water and the same volume of absolute ethanol to wash once according to the above steps, and dry thoroughly under vacuum to obtain poly-p-styrene precursor-coated microspheres;

[0036]2. Add the microspheres obtained in step 1 into 3 mL of xylene solution, and add 0.2 mL of triethylamine, and the reaction is carried out under anhydrous and oxygen-free conditions. The reaction was shaken at 120°C for 3 hours. After the solution was cooled, 5 mL of acetone was added, centrifuged, and the supernatant was removed. 8 mL of acetone was added to the microspheres for shaking for 1 hour, and the supernatant was removed by centrifugation. Then wash once with the same volume of d...

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Abstract

The invention discloses a high-molecular fluorescent microsphere with controllable emission wavelength and a preparation method thereof and belongs to the fluorescent sensing material preparation field. The preparation method comprises the following steps of: adding the microsphere, the surface of which is adsorbed with a para-phenylene vinylene precursor, into a xylene solution under anhydrous and anoxybiotic conditions; adding a catalyst p-toluene sulphonic acid or a protective agent triethylamine, carrying out elimination reaction under vacuum condition at the temperature of 60-140 DEG C, and controlling the number of a tetrahydrothiophene group substituted by a methylol group in the polymer precursor to obtain the conjugated high-molecular fluorescent microsphere with different emission wavelengths. By the adoption of the flexible polymer precursor, it has good dissolvability in water, is stable in performance after being absorbed on the surface of the microsphere to perform elimination reaction and form a double bond, and has good antisolvent dissolvability. Therefore, the polymer precursor has good dissolvability and is easy to mold and process, and simultaneously performance stability of the polymer after elimination is also guaranteed.

Description

technical field [0001] The invention relates to a microsphere loaded with fluorescence-emitting poly(p-phenylene) conjugated polymer on the surface and a preparation method thereof, which belongs to the field of preparation of fluorescence sensing materials. Background technique [0002] Fluorescent microspheres are a new type of fluorescent sensing material with good application prospects. Studies have shown that it can be used in many biomedical related fields, such as gene sequencing and expression, protein sequencing, disease diagnosis and drug screening, in environmental monitoring and Food inspection and other aspects also have a lot of demand, and at the same time, it also provides a basis for the development of another cutting-edge technology-liquid chip suspension technology. [0003] Before the present invention was made, the Chinese invention patent (CN 101824191 A) disclosed a polymer fluorescent microsphere and its preparation method, using polystyrene (PS) cros...

Claims

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Application Information

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IPC IPC(8): C08L25/04C08L65/00C09K11/06
Inventor 范丽娟赵伟宋婧陈云
Owner SUZHOU UNIV
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