Immunofluorescence dipstick component for quickly and quantitatively detecting protein of plurality of types and detection card component prepared from same and preparation method thereof

Abstract

The invention discloses an immunofluorescence dipstick component for quickly and quantitatively detecting protein of a plurality of types and a detection card component prepared from the same and a preparation method thereof, wherein the protein of a plurality of types comprises muscle hemoglobin / creatine kinase isoenzyme / troponin I; the dipstick component comprises a dipstick consisting of a bottom liner, a water-absorbing pad, a coated analysis membrane and a sample pad and independently packaged fluorescein mark specific antibodies; three detection lines and a quality control line are arranged on the coated analysis membrane; the detection line on the coated analysis membrane is respectively coated with an anti-myoglobin monoclonal antibody line, an anti-creatine kinase isozyme monoclonal antibody line and a troponin I monoclonal antibody line; the quality control line is coated with a rabbit IgG antibody; the fluorescein mark specific antibodies comprise the anti-myoglobin monoclonal antibody line, the anti-creatine kinase isozyme monoclonal antibody line, an troponin I monoclonal antibody and an anti-rabbit IgG antibody; and the detection card component comprises the dipstick, a card box consisting of a cover plate and a back plate and independently packaged platinum porphyrin mark specific antibodies and can synchronously detect the muscle hemoglobin / creatine kinase isoenzyme / troponin I, is simple to operate, is quick and sensitive, and has good specificity.

Description

technical field [0001] The invention belongs to the field of medical testing, and in particular relates to an immunofluorescence test strip component for rapid quantitative and combined determination of myoglobin / creatine kinase isoenzyme / troponin I and a preparation method thereof. Background technique [0002] Coronary heart disease has become a major disease affecting the health of the population. Acute myocardial infarction (AMI) is the main cause of death in coronary heart disease patients. How to timely discover patients with acute myocardial infarction and corresponding treatment is the key to saving the lives of patients with myocardial infarction. Traditional diagnosis is mainly based on typical clinical manifestations, ECG changes and laboratory enzyme examination. However, a considerable number of patients with myocardial infarction have no obvious clinical manifestations, and there is no obvious change in the early ECG. In this case, the application of specifi...

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Problems solved by technology

3) It lasts for a long time in the blood. If it appears transiently or exists in the blood for a short time, it is difficult to seize the right time to detect a positive result, which may easily lead to missed diagnosis

[0008] At present, the detection of myoglobin, CK-MB and cTnI mostly adopts chemiluminescence method, which is labeled with chemiluminescent substances as detection antibodies, which greatly improves the accuracy and sensitivity of the determination, but requires expensive detection equipment, which is not conducive to clinical promotion , especially in primary hospitals

Although the current bedside detection method (colloidal gold test strip method) can meet the requirements of clinical rapid and combined detection of multiple myocardial markers, especially the needs of primary hospitals, but because it is only a qualitative detection, it cannot meet the requirements of accurate and quantitative clinical diagnosis. Requirements, unable to play a good role in monitoring the curative effect on the course of AMI

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