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Pharmaceutical composition and usesthereof

A composition and drug technology, applied in drug combinations, antitumor drugs, pharmaceutical formulations, etc., can solve the problems of drug research and development restrictions, lack of preventive and therapeutic effects, etc., and achieve the effect of fast analysis and cost saving

Active Publication Date: 2012-09-26
WUHAN HUADA PHARMA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Humans are the only host of HPV, so it is impossible to carry out culture and expansion experiments in vitro and in animal models, which greatly limits the development of drugs for the prevention or treatment of HPV infection, resulting in no effective prevention and treatment worldwide Drugs for HPV infection, although foreign countries have developed vaccines that can prevent HPV16 and 18 subtype infections, they have the following defects: they can only be used for asexual women aged 9-24 who are not infected with HPV16 and 18, and for 16 and 18 Infections of subtypes other than subtypes have no preventive and therapeutic effects
[0005] At present, the drugs for the treatment of cervical diseases, vulvar intraepithelial neoplasia, warts and skin precancerous lesions caused by HPV infection still need further research and improvement

Method used

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  • Pharmaceutical composition and usesthereof
  • Pharmaceutical composition and usesthereof
  • Pharmaceutical composition and usesthereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1: Optimization of the preparation process of golden buckwheat extract

[0080] In this example, through a large number of experiments, the method for preparing the golden buckwheat extract, that is, the extraction process and purification process in the preparation process of the golden buckwheat extract has been studied, and the optimal extraction process and purification process have been screened out, thereby completing the analysis of the golden buckwheat extract. Optimization of extract preparation process.

[0081] 1. Optimization of golden buckwheat extraction process

[0082] Using the orthogonal test method, ethanol concentration (A factor), solid-liquid ratio (B factor), extraction times (C factor) and extraction time (D factor) were used as the experimental factors of the gold buckwheat extraction process optimization test, and each factor was taken as Three levels, work out the factor level table of gold buckwheat extraction process optimization ex...

Embodiment 2

[0138] Example 2: Optimization of the preparation process of bark bark extract

[0139] In this embodiment, through a large number of experiments, the method for preparing the extract of Cortex chinensis, that is, the extraction process and purification process in the preparation process of the bark bark extract has been studied, and the optimal extraction process and purification process have been screened out, thereby completing the preparation of the bark bark extract. Optimization of the preparation process.

[0140] 1. Optimization of extraction conditions

[0141] Using the method of orthogonal experiment, ethanol concentration (A factor), solid-liquid ratio (B factor), extraction times (C factor), and extraction time (D factor) were used as the experimental factors of Qinpi extraction process optimization test, and each factor was taken as Three levels, work out the factor level table of Qinpi extracting process optimization experiment, see table 11, then based on tabl...

Embodiment 3

[0159] Example 3: Effects of Golden Buckwheat Extract, Qinpi Extract and Pharmaceutical Composition on Expression of HPV18 E7 Gene in Human Cervical Cancer HeLa Cells

[0160] 1. Materials

[0161] Take HeLa cells in the logarithmic growth phase, and use 1×10 6 Inoculate in a six-well plate at a density of 1 / ml, 1ml per well, at 37°C, 5% CO 2 , cultured under saturated humidity conditions for 24h, as the experimental cell line.

[0162]Test drug: golden buckwheat extract (prepared according to Example 1), Qinpi extract (prepared according to Example 2), pharmaceutical composition (the golden buckwheat extract and Qinpi extract were respectively prepared into mother liquor, and then the mother liquor was prepared in proportion to mixed to prepare a pharmaceutical composition); positive drug: acyclovir.

[0163] 2. Experimental method and experimental results

[0164] To the HeLa cells cultured for 24 hours, different concentrations of test drugs with cytotoxicity less than ...

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Abstract

The invention relates to a pharmaceutical composition and uses thereof. The pharmaceutical composition comprises a golden buckwheat rhizome extract and an ash bark extract. The golden buckwheat rhizome extract is prepared by the following steps: carrying out an alcohol-added extraction on golden buckwheat rhizome to obtain an extract solution of the golden buckwheat rhizome, and purifying and drying the extract solution of the golden buckwheat rhizome to obtain the golden buckwheat rhizome extract. The ash bark extract is prepared by the following steps: carrying out an alcohol-added extraction on ash bark to obtain an extract solution of ash bark, and purifying and drying the extract solution of the ash bark to obtain the ash bark extract. The pharmaceutical composition of the invention can be used in treatment and prevention of diseases caused by HPV infection, including cervical diseases, vaginal intraepithelial neoplasia, verrucosis, and precancerous lesion of skin cancer.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a pharmaceutical composition and its application. More specifically, the present invention provides a pharmaceutical composition, the use of the pharmaceutical composition in preparing a drug, a method for preparing a drug, a drug, and a method for detecting the pharmaceutical composition or drug. Background technique [0002] Human papilloma virus (HPV) widely exists in nature and has a high degree of tissue and host specificity. It is a type of DNA virus that can cause abnormal proliferation of human skin and mucous membranes, and cause verrucous lesions and papillomas in host tissues. . So far, at least 120 different HPV subtypes have been found, of which more than 50 subtypes can infect the genital tract mucosa, and more than 19 have been confirmed to be closely related to cervical cancer. According to the relationship between HPV subtypes and reproductive tract ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/70A61P31/20A61P15/00A61P35/00G01N30/02G01N30/06
Inventor 张思佳黄璐陈顺钦牟峰黄伟汪建
Owner WUHAN HUADA PHARMA
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