Rice blast disease-resisting protein, coding gene and application thereof

Abstract

The invention discloses a rice blast disease-resisting protein, a coding gene and an application thereof. The protein provided by the invention is following (a) or (b): (a) a protein composed of amino acid sequences shown in the sequence 2 in the sequence table; (b) a protein obtained by substituting and / deleting and / adding one or more of amino acid residue for the amino acid sequence shown in the sequence 2 and deriving from the (a) related to plant blast disease resistance. The PID3-A4 protein and the coding gene provided by the invention can be used for cultivating a new blast disease-resisting plant variety, particularly, a variety showing resistance for blast disease caused by at least one of following Magnaporthe oryzae microspecies: JS2001-108-1, Zhong10-8-14, 97-27-2, 03-10-66-1, 07-31-1-2, 03-10-76-3, 10-25-1-1, 10-32-2-1, 03-11-37-1, 07-55-1-1, 10-47-9-2, 10-120-21-2, 07-21-1-1, 10-62-2-1, 07-26-22, 09-87-2-1, Y34, 99-26-2, CH706 and 99-20-2. The protein is beneficial to improve rice variety and has an important significance for enlarging crop planting range and improving crop yield.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and relates to a rice blast resistance protein, its coding gene and its application. Background technique [0002] Plants will be attacked by various pathogenic microorganisms during the growth and development process. Although plants do not have the ability to actively "seek advantages and avoid disadvantages", during the long-term co-evolution process with various pathogenic microorganisms, plants have formed a precise response to pathogenic microorganisms. Signal perception and defense system. Plant innate immunity mainly includes three aspects: basic resistance, resistance mediated by disease resistance genes, and systemic acquired resistance. Among them, the resistance mediated by disease resistance genes is an important part of plant disease resistance due to its fast response time and strong disease resistance response, often accompanied by hypersensitivity reactions at the point ...

AI Technical Summary

Problems solved by technology

[0005] At present, the cloning of the rice blast resistance gene mainly relies on map-based cloning. Although both the indica rice 9311 and the japonica rice Nipponbare have complete genome sequences, which brings great convenience to the map-based cloning of rice genes, the cloning of the rice blast resistance gene is still difficult. said, there are still many difficulties

For example, the identification of rice blast resistance of each individual plant in a fine-mapping population is not only a huge workload, but also the incidence of rice blast in the field is easily affected by the environment, resulting in statistical errors in traits and accurate positioning; in addition, from the sequenced According to the 9311 and Nipponbare genome sequences, most of the NBS-LRR genes exist in clusters. Even if the target gene can be located in a certain segment, it is difficult to judge which of the clustered genes is the real disease resistance gene; Furthermore, the genetic background of currently used rice varieties is narrow, and there are not many blast resistance genes available.

However, wild rice contains a wealth of disease-resistant gene resources. Since the research on wild rice is still in its infancy, there are no fine genetic maps and physical maps, and it is obviously difficult to clone the rice blast resistance gene using map sites.

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