Rice blast disease-resisting protein, coding gene and application thereof

A rice blast resistance and encoding technology, which is applied in the fields of application, genetic engineering, and plant gene improvement, can solve problems such as the difficulty of judging clustered gene resistance genes, few blast resistance genes, and heavy workload

Inactive Publication Date: 2012-10-03
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the cloning of the rice blast resistance gene mainly relies on map-based cloning. Although both the indica rice 9311 and the japonica rice Nipponbare have complete genome sequences, which brings great convenience to the map-based cloning of rice genes, the cloning of the rice blast resistance gene is still difficult. said, there are still many difficulties
For example, the identification of rice blast resistance of each individual plant in a fine-mapping population is not only a huge workload, but also the incidence of rice blast in the field is easily affected by the environment, resulting in statistical errors in traits and accurate positioning; in addi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rice blast disease-resisting protein, coding gene and application thereof
  • Rice blast disease-resisting protein, coding gene and application thereof
  • Rice blast disease-resisting protein, coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0057] Example 1. Obtaining of Pid3-A4 resistance gene

[0058] This example uses the sequence information of the rice blast resistance gene Pid3 cloned from the rice land cultivated valley to isolate and clone the rice blast resistance homologous gene in Oryza rufipogon A4.

[0059] 1. Obtaining the sequence information of rice blast resistance gene Pid3

[0060] The rice blast resistance gene Pdi3 was cloned from rice land cultivars. It has good resistance to the rice blast fungus race in the japonica rice area. The identification race used to locate the clone is the rice blast fungus race in the japonica rice area. zhong-10-8-14. Obtained from NCBI (http: / / www.ncbi.nlm.nih.gov) the complete gene sequence of the rice blast resistance gene Pid3 (GenBank: FJ745364.1, positions 3010-5784). The full length of the Pid3 gene is 2775bp. Contains introns and encodes an N-terminal non-TIR, non-CC NBS-LRR protein with a conservative motif: RSLALSIEDVVD (78-89aa). The NBS domain is encoded...

Example Embodiment

[0076] Example 2. Obtainment of Transgenic Rice with Pid3-A4 Gene and Study on Its Function

[0077] 1. Obtainment of Pid3-A4 Transgenic Rice

[0078] 1. Construction of recombinant expression vector PZH01-pid3-A4

[0079] The PCR product obtained in Example 1 (TT TCTAGA +Sequence 1+ GGTACC AG) After digestion with Xba Ⅰ and Kpn Ⅰ, the fragment obtained is the same as the plant binary expression vector PZH01 (Xiao, H., Wang, Y., Liu, D., Wang, W., Li, X. ,Zhao,X.,Xu,J.,Zhai,W.and Zhu,L.(2003)Functional analysis of the rice AP3 homologue OsMADS16 by RNA interference.Plant Mol.Biol.52,957-966. The public can inherit from the Chinese Academy of Sciences Connect with the vector fragment obtained by the Institute of Developmental Biology, and transform the ligation product into Escherichia coli DH5a to obtain a transformant. The transformant is shaken, the plasmid is extracted, and the sample is sent for sequencing. Sequencing indicated that the recombinant plasmid with the Pid3-A4 g...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a rice blast disease-resisting protein, a coding gene and an application thereof. The protein provided by the invention is following (a) or (b): (a) a protein composed of amino acid sequences shown in the sequence 2 in the sequence table; (b) a protein obtained by substituting and/deleting and/adding one or more of amino acid residue for the amino acid sequence shown in the sequence 2 and deriving from the (a) related to plant blast disease resistance. The PID3-A4 protein and the coding gene provided by the invention can be used for cultivating a new blast disease-resisting plant variety, particularly, a variety showing resistance for blast disease caused by at least one of following Magnaporthe oryzae microspecies: JS2001-108-1, Zhong10-8-14, 97-27-2, 03-10-66-1, 07-31-1-2, 03-10-76-3, 10-25-1-1, 10-32-2-1, 03-11-37-1, 07-55-1-1, 10-47-9-2, 10-120-21-2, 07-21-1-1, 10-62-2-1, 07-26-22, 09-87-2-1, Y34, 99-26-2, CH706 and 99-20-2. The protein is beneficial to improve rice variety and has an important significance for enlarging crop planting range and improving crop yield.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and relates to a rice blast resistance protein, its coding gene and its application. Background technique [0002] Plants will be attacked by various pathogenic microorganisms during the growth and development process. Although plants do not have the ability to actively "seek advantages and avoid disadvantages", during the long-term co-evolution process with various pathogenic microorganisms, plants have formed a precise response to pathogenic microorganisms. Signal perception and defense system. Plant innate immunity mainly includes three aspects: basic resistance, resistance mediated by disease resistance genes, and systemic acquired resistance. Among them, the resistance mediated by disease resistance genes is an important part of plant disease resistance due to its fast response time and strong disease resistance response, often accompanied by hypersensitivity reactions at the point ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H5/00
CPCY02A40/10
Inventor 朱立煌吕启明徐筱江光怀李晓兵李仕贵徐吉臣
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap