Method for improving biogas yield through plant fermentation

Abstract

The invention discloses a method for improving the biogas yield through plant fermentation, pertaining to the biotechnological field. Lignin peroxidase gene and manganese peroxidase gene of white rot fungi, laccase gene of bacillus subtilis and endoglucanase enzyme gene, dextran exonuclease gene and beta glucosidase gene of trichoderma are first cloned. An Escherichia coli -bacillus subtilis shuttle plasmid which comprises the above six-gene expression frame is built and the bacillus subtilis is transformed by the plasmid to obtain engineering bacteria; and the bacillus subtilis engineering bacteria are applied in a pretreating process of the plant raw material fermentation for biogas, and in combination with natural biogas fermentative microorganisms, the bacillus subtilis engineering bacteria are applied in biogas production using the fermentative plant, which significantly improves the plant raw material utilization rate and the biogas yield. With the method of the invention, the problem that lignin and celluloses left in the plant fermentation for the biogas cannot be effectively hydrolyzed is solved, and development of an industry which uses plant fermentation for the biogas is promoted.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the construction of a lignin peroxidase gene expression framework, a manganese peroxidase gene expression framework, a laccase gene expression framework, an endoglucanase gene expression framework, and an exoglucan gene expression framework. The Escherichia coli-Bacillus subtilis shuttle plasmid of the enzyme gene expression framework and the β-glucosidase gene expression framework, the Bacillus subtilis engineering bacterium and the method for improving the biogas production of plant fermentation by using the engineering bacterium. Specifically, an application carrying a lignin peroxidase gene expression framework, a manganese peroxidase gene expression framework, a laccase gene expression framework, an endoglucanase gene expression framework, and an exoglucanase gene expression framework The Bacillus subtilis engineering bacteria of the Escherichia coli-Bacillus subtilis shuttle plasmi...

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the lignin and cellulose of the current plant raw material fermentation biogas cannot be hydrolyzed effectively. Function, through molecular biology techniques, construct lignin peroxidase gene expression framework, manganese peroxidase gene expression framework, laccase gene expression framework, glucan endoglucanase gene expression framework, dextran exo The Bacillus subtilis engineering bacteria of the Escherichia coli-Bacillus subtilis shuttle plasmid of the enzyme gene expression framework and the β-glucosidase gene expression framework, and provide a kind of Bacillus subtilis engineering bacteria to carry out the pretreatment of plant raw material biogas fermentation and the combination with natural Method of co-anaerobic digestion of plants with biogas fermentation microorganisms to produce biogas

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