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Avermectin pesticide residual degrading bacterium, and microbial inoculum produced thereby

A technology for pesticide residue degradation and abamectin, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of abamectin residue degradation agent products, etc., to improve the quality of agricultural products, easy to use, and low cost Effect

Inactive Publication Date: 2012-10-17
BIOLOGY INST OF SHANDONG ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few research reports on the degradation of AVMs residues in the academic circles, and there is no abamectin residue degradation agent product that can be industrialized and used on a large scale in the market.

Method used

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  • Avermectin pesticide residual degrading bacterium, and microbial inoculum produced thereby
  • Avermectin pesticide residual degrading bacterium, and microbial inoculum produced thereby
  • Avermectin pesticide residual degrading bacterium, and microbial inoculum produced thereby

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the isolation of degrading bacterial strain

[0025] Take 10g of abamectin fermentation waste residue sample and add it into a conical flask filled with 90mL sterile water (250mL size, glass beads inside). Shake for 15 minutes, after static clarification, take 1 mL of supernatant and add 99 mL of basal medium, place them in incubators at 40°C, 50°C, and 60°C respectively, and culture with shaking at 150r / min. 3 days as a cycle for acclimatization, a total of 5 cycles, the concentration of abamectin is set as: 25mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, gradually increasing in each acclimation cycle. Each acclimatization medium was mixed with 10 -1 、10 -2 and 10 -3 Spread the basic medium plate (as a control) and the selective medium plate containing abamectin at dilution, select a single colony that grows fast and can use abamectin as the sole carbon source for further isolation and purification, until there is a single colony Appear.

[0026] After ...

Embodiment 2

[0029] Embodiment 2: bacterial strain AZ11 is to the degradation activity of abamectin

[0030] The abamectin efficient degrading strain AZ11 was cultured in liquid fermentation at 40°C and 150r / min, and the number of bacteria was adjusted to 10 9 cfu / mL, made into bacterial suspension. Add 10g / L glucose, peptone and yeast extract to the basal salt medium containing 100mg / L abamectin to study the effect of adding exogenous nutrients on the degradation of abamectin by AZ11, inoculate at 1% Bacterial suspension, cultured for 48h after sampling to determine the content of abamectin in the sample. In addition, add abamectin solution to the basal salt medium (with 500 mg / L glucose) to make the concentration to 100, 200, 300, 400, 500, 600 and 700 mg / L, and inoculate AZ11 with 1% inoculum Bacterial suspension, cultured at 50°C for 36 hours, then sampled to determine OD 660 value, to study the tolerance of strain AZ11 to abamectin.

[0031] Results from figure 1It can be seen...

Embodiment 3

[0033] Embodiment 3: the preparation of avermectin pesticide residue degradation bacterial agent

[0034] Activate the slant-preserved species of abamectin-degrading bacterium AZ11, inoculate it in LB liquid medium, shake it at 40-45°C to the logarithmic growth phase, inoculate the seed fermentation tank, and the formula of the seed fermentation tank is: corn flour 2%; Bean cake powder 1%; glucose 0.5%; K 2 HPO 4 0.4%; MgSO 4 ·7H 2 O 0.02%; CaCO 3 0.2%; pH 7.2-7.5. Autoclaved at 121°C, and when cooled to about 45°C, inoculate 10% of the cultured strain in the shake flask into a 10L seed tank, and cultivate to the logarithmic growth phase. The fermented liquid of the seed fermenter was inoculated into the production fermenter with 10% inoculum, and the medium formula was: corn flour 3%; bean cake flour 2%; CaCO 3 0.5%;K 2 HPO 4 0.2%; ZnSO 4 ·5H 2 O 0.05%; MgSO 4 ·7H 2 O 0.05%; pH 7.2;

[0035] The culture conditions of the above fermentation tanks are: culture...

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Abstract

The invention discloses an avermectin pesticide residual degrading bacterium and a microbial inoculum produced thereby, and relates to separation and screening of Bacillus stearothermophilus AZ11, a microbial inoculum produced through fermenting the Bacillus stearothermophilus AZ11, and an application of the microbial inoculum. Above bacterial strain is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC NO.6119. The avermectin pesticide residual degrading microbial inoculum produced through fermenting the bacterial strain AZ11 can be widely applied to the restoration of soil and water subjected to avermectin pesticide residual pollution, can also be applied to the degradation of avermectin residuals in avermectin fermentation waste residues or other organic fertilizer raw materials, and can be directly sprayed on surfaces of soil or plants in the agricultural production to effectively degrade avermectin pesticide residuals, so the use is convenient and the cost is low; and the microbial inoculum can also be directly applied to the fermenting production process of the avermectin waste residues to realize the harmless resource utilization of waste residue fermentation products.

Description

technical field [0001] The invention relates to an abamectin pesticide residue degrading bacterium and a bacterial agent produced therefrom, which belong to the field of biotechnology and are suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. Background technique [0002] Pesticides are essential to prevent and control diseases and insect pests and increase crop yields in the process of agricultural production. However, while pesticides are widely used, they also cause serious pollution to the environment, making the contradiction between agricultural yields and environmental quality increasingly prominent. Many pesticides are harmful to the environment. Humans and non-target animals have endocrine disrupting effects and "three-causing" effects (carcinogenic, teratogenic and mutagenic). [0003] Avermectins (AVMs for short) are produced by actinomycetes ( Streptomyces avermitilis ) produced a group...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34A62D3/02C12R1/07A62D101/04A62D101/28
Inventor 魏艳丽李纪顺陈凯周红姿李红梅杨合同
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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