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Quantitative detection method of aroma components in yellow serofluid

A technology for quantitative detection of aroma components, which is applied in the field of detection analysis and quantitative detection of aroma components in yellow pulp water, can solve the problems of cumbersome operation, difficult quantification, inaccuracy, etc., and achieve the goals of preventing quantitative distortion, protecting the safety of use, and overcoming discrimination effects Effect

Active Publication Date: 2012-10-17
安徽润安信科检测科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen from the above that the SDE method takes a long time to extract, and the operation is relatively cumbersome; the SPME method is relatively simple and fast, and the sample will not be destroyed, but SPME has a significant competitive adsorption effect on multi-component analysis, so quantification is difficult.
[0006] According to the above-mentioned reported literature, the detection methods of volatile aroma components in yellow pulp water are all determined by gas chromatography, but the pretreatment methods are different, and most of them have problems such as cumbersome operation, easy contamination of gas chromatographic columns, or inaccurate quantification.

Method used

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  • Quantitative detection method of aroma components in yellow serofluid
  • Quantitative detection method of aroma components in yellow serofluid
  • Quantitative detection method of aroma components in yellow serofluid

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Experimental program
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Embodiment 1

[0031] 1. Pretreatment: Use a 25ml pot-bellied straw to absorb 25ml of yellow pulp water, put it in a 50ml volumetric flask, and then add ethanol solution with a volume concentration of 96% to make up to volume. Fully mix the yellow pulp water and alcohol to obtain a mixed solution, and make it sealed and fully precipitated for 0.5h. The mixed solution is subjected to airtight coarse filtration with qualitative filter paper to obtain a relatively clear crude filtrate, and the crude filtrate is filtered with a 0.45 μm microporous membrane to obtain a pretreatment solution.

[0032] 2. Detection: draw 10ml of pretreatment solution, then add 0.1ml of mixed internal standard, mix well and analyze by gas chromatography. The detection conditions of gas chromatography detection are:

[0033] Chromatographic column: CP-WAX57CB capillary column 50m×0.25mm×0.20μm from American Varian Chrompack Company;

[0034] Column temperature: second-stage temperature program, the initial temperat...

Embodiment 2

[0047] 1. Pretreatment: Use a 25ml pot-bellied straw to draw 25ml of yellow pulp water, put it in a 50ml volumetric flask, then add ethanol solution with a volume concentration of 96% to constant volume, mix evenly to obtain a mixed solution, seal the mixed solution and fully precipitate it for 0.5h , and then centrifuged, the centrifugal speed is 10000r / min, the centrifugation time is 10min, and the supernatant is collected. The supernatant was filtered with a 0.45 μm microporous membrane to obtain a pretreatment solution.

[0048] 2, detection: detection method is the same as embodiment 1.

[0049] The aroma components and content of the yellow jelly water are shown in Table 2. Because the detection liquid is the yellow jelly water after volume dilution of 1:1, the content of the aroma components in the actual yellow jelly water is twice the test result. Using this pretreatment method combined with centrifugation and microfiltration, the gas chromatogram of Huangjiangshui N...

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Abstract

The invention discloses a quantitative detection method of aroma components in yellow serofluid, comprising pretreatment and unit detection processes. During the pretreatment process, an ethanol solution with its volume concentration being not less than 95% and yellow serofluid are uniformly mixed according to the volume ratio of 1:1 to obtain a mixed liquor; the mixed liquor is sealed and stands for 20-40 min, and then qualitative filter paper is used for coarse filtration and a coarse filtrate is collected or centrifugation is conducted and a supernatant is collected; the coarse filtrate or the supernatant is filtered by the use of a microporous membrane of 0.45 microns and the filtrate is collected to obtain a pretreatment fluid. According to the detection process, a mixed internal standard of 0.1 mL is added into the pretreatment fluid of 10 mL, and gas chromatography detection is carried out after uniform mixing, wherein the mixed internal standard is a mixture of tertiary amyl alcohol, n-amyl acetate and 2-ethylbutanoic acid which are mixed according to the volume ratio of 1: 1: 1. The technical scheme of the present invention is simple and rapid. In addition, by the adoption of the method, service safety of a gas chromatographic column can be effectively protected, and the service life of the gas chromatographic column is prolonged.

Description

1. Technical field [0001] The invention relates to a detection and analysis method, in particular to a method for quantitative detection of aroma components in yellow jelly water, belonging to the technical field of wine by-product analysis. 2. Background technology [0002] Yellow pulp water is an accompanying organism in the fermentation process of liquor brewing. The color is brownish yellow. Yellow pulp water contains rich acids, esters, alcohols, aldehydes and other aroma substances, as well as starch, sugar, solids and caproic acid that has been domesticated for a long time. Bacteria, butyric acid bacteria, yeast and other beneficial microorganisms. Yellow pulp water contains a lot of beneficial flavor components, and extracting the flavor components in it for liquor blending is the development direction of the liquor industry in the future. Because the yellow pulp water contains starch, sugar, microorganisms and other substances, the determination of the aroma compon...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/14
Inventor 周庆伍李安军汤有宏刘国英高江婧万春环孙大春王录
Owner 安徽润安信科检测科技有限公司
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