Preparation technology of polystyrene microsphere for reinforcing turbidimetric reagent

Abstract

The invention relates to a preparation technology of a polystyrene microsphere for reinforcing a turbidimetric reagent. A reaction system of the preparation technology comprises an external water phase, a polymeric monomer and a functional monomer, wherein the external water phase comprises 0.1-2g of sodium dodecyl sulfate, 2-16g of polyethylene glycol 6000, 0.1-3g of sodium chloride and 400-600mL of purified water; the polymeric monomer comprises 50-100mL of styrene and 0.5-1.0mL of divinyl benzene; and the functional monomer comprises 2-16mL of ethyl acrylate and 10-60mL of a boric acid andborax buffer solution. Carboxylated polystyrene microsphere is obtained through configuration of the external water phase, purification of the polymeric monomer and preparation and polymerization reaction of an emulsion. According to the polystyrene microsphere produced through the preparation technology, the cost is 1% of the price of microspheres in foreign companies, the anti-agglutination effect is more than ten times better than that of the microspheres in foreign companies, the cross-linking efficiency with antibodies is improved by 50%, and the multi-purpose microsphere can satisfy requirements of a plurality of projects.

Description

technical field [0001] The field of polymer microsphere materials of the present invention, in particular, relates to a preparation process of polystyrene microspheres applied to enhanced turbidimetric reagents. Background technique [0002] The preparation and application of polystyrene microspheres have developed rapidly. Not only have many new technologies been developed on the basis of traditional emulsion polymerization and suspension polymerization, but also the performance and variety of microspheres have become more and more perfect. Dispersion, particle size controllable, various forms, structures, and high-performance products are widely used in the fields of standard measurement, food chemical industry, medicine, bioengineering, information engineering and microelectronics technology, especially in the field of biomedicine. It is of great significance to the research of clinical diagnosis, testing, immune technology, cytology, etc. In the in vitro diagnostic reag...

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Problems solved by technology

[0004] Polystyrene microspheres are one of the important raw materials for enhancing turbidimetric reagents. The use of microspheres from foreign companies will lead to an increase in other raw materials for the production of reagents and an increase in reagent costs.

At the same time, there are many problems in the process of applying the microspheres of foreign companies to enhanced turbidimetric reagents, such as easy aggregation, low cross-linking efficiency with antibodies, and difficulty in improving the production efficiency of reagents.

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