Microorganism collection method in ecological environment survey

A collection method and ecological environment technology, applied in the direction of fungi, etc., can solve problems such as difficult to maximize, difficult to screen mycorrhizal flora growing in the wild, difficult to isolate spores, etc.

Active Publication Date: 2012-10-31
CHINA SHENHUA ENERGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many unfavorable factors in using a single spore as a bacterial source. First, the spore infection rate is moderate, and the number of vegetative species accumulates slowly; Easy separation of spores; at the same time larger or smaller particles may be lost during wet sieving
Although pure species can be obtained by using spores as propagules, it is difficult to maximize the role of bacterial agents produced by a single bacterial source in nature, due to changes in the field environment, antagonism between strains, and the selection of strains by host plants Sexuality, etc. wi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: (In situ collection of arbuscular mycorrhizal fungi in the collapsed area of ​​Huojitu Mine, Yulin, Shaanxi)

[0029] A nylon cloth with a pore size of 30 μm is selected as the material of the bacteria bag. The fungus bag has an antiseptic function. During a growing season of the host plant, the fungus bag will not rot in the soil. The length of the bacteria bag is 10cm, the width is 8cm, and the thickness of the bacteria bag is 3cm; one end of the bacteria bag is provided with an opening; the middle of the opening end is provided with a traction rope.

[0030] The test substrate was collected at Gaojiapan, Daliuta Town, Shenmu County, Yulin, Shaanxi, belonging to the sinking area of ​​the Huojitu Mine in Shendong Mining Area. The test substrate was the degraded soil in the sinking area and passed through a sieve with a diameter of 1mm. The sieved soil is sterilized by high temperature and high pressure steam (121°C, 2h) before being put into the above-mentioned ...

Embodiment 2

[0035] Example 2: (In-situ collection of arbuscular mycorrhizal fungi in the subsidence area of ​​Bulianta Mine, Shendong Mining Area)

[0036] A nylon cloth with a pore size of 30 μm is selected as the material of the bacteria bag. The fungus bag has an antiseptic function. During a growing season of the host plant, the fungus bag will not rot in the soil. The length of the bacteria bag is 10cm, the width is 8cm, and the thickness of the bacteria bag is 3cm; one end of the bacteria bag is provided with an opening; the middle of the opening end is provided with a traction rope.

[0037] The test site is the Bulianta mine in Shendong mining area. The test substrate is the degraded soil in the subsidence area of ​​the mine, and it is passed through a sieve with an aperture of 1mm. The sieved soil is sterilized by high temperature and high pressure steam (121℃, 2h). Air dry.

[0038] The test substrate is put into the above-mentioned bacterial bag, and each bacterial bag is filled wit...

Embodiment 3

[0042] Example 3: (In-situ collection of arbuscular mycorrhizal fungi in Ningxia coal gangue hill reclamation area)

[0043] A nylon cloth with a pore size of 30 μm is selected as the material of the bacteria bag. The fungus bag has an antiseptic function. During a growing season of the host plant, the fungus bag will not rot in the soil. The length of the bacteria bag is 10cm, the width is 8cm, and the thickness of the bacteria bag is 3cm; one end of the bacteria bag is provided with an opening; the middle of the opening end is provided with a traction rope.

[0044] The test site is the coal gangue hill of Dawukou Coal Washing Plant in Ningxia, which is located in Shizuishan City, Ningxia Hui Autonomous Region. It belongs to a mid-temperate arid climate zone with sparse and concentrated precipitation, sufficient sunlight, strong evaporation, and dry air, which is extremely unfavorable for plant growth. In this test site, the coal gangue hill was selected. The coal gangue hill wa...

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Abstract

The invention relates to a microorganism collection method in an ecological environment survey. The microorganism for the microorganism collection method is arbuscular mycorrhizal fungi. The collection method comprises the following steps: manufacturing a collection culture bag, preparing experimental matrixes, and packaging the experimental matrixes to the culture bag and sealing, and launching the culture bag. By the microorganism collection method in the ecological environment survey, the culture bag can be enriched in spore and mycelium, and the screening range is narrowed greatly and the workload is reduced.

Description

Technical field [0001] The invention relates to a method for collecting microorganisms, in particular to a method for collecting microorganisms in ecological environment investigation. Background technique [0002] Arbuscular mycorrhizal fungus (AMF) is a ubiquitous endosymbiotic fungus, which can form symbiotic bodies with more than 80% of land plants. Arbuscular mycorrhizal fungi play a vital role in maintaining the stability of the plant rhizosphere ecosystem, promoting plant growth, improving host plant resistance and improving the quality of degraded soil. Arbuscular mycorrhizal fungi are composed of mycorrhizal spores (fruits), arbuscular bodies, vesicles, and hyphae, all of which can be used as propagules. At present, the separation and purification of mycorrhiza mainly uses spores as donors, and the microspore fruit, spores and soil auxiliary cells in the soil are usually collected by wet sieve method. There are many disadvantages in using a single spore as a source of ...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 毕银丽张凯
Owner CHINA SHENHUA ENERGY CO LTD
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