Microorganism collection method in ecological environment survey

Abstract

The invention relates to a microorganism collection method in an ecological environment survey. The microorganism for the microorganism collection method is arbuscular mycorrhizal fungi. The collection method comprises the following steps: manufacturing a collection culture bag, preparing experimental matrixes, and packaging the experimental matrixes to the culture bag and sealing, and launching the culture bag. By the microorganism collection method in the ecological environment survey, the culture bag can be enriched in spore and mycelium, and the screening range is narrowed greatly and the workload is reduced.

Description

Technical field [0001] The invention relates to a method for collecting microorganisms, in particular to a method for collecting microorganisms in ecological environment investigation. Background technique [0002] Arbuscular mycorrhizal fungus (AMF) is a ubiquitous endosymbiotic fungus, which can form symbiotic bodies with more than 80% of land plants. Arbuscular mycorrhizal fungi play a vital role in maintaining the stability of the plant rhizosphere ecosystem, promoting plant growth, improving host plant resistance and improving the quality of degraded soil. Arbuscular mycorrhizal fungi are composed of mycorrhizal spores (fruits), arbuscular bodies, vesicles, and hyphae, all of which can be used as propagules. At present, the separation and purification of mycorrhiza mainly uses spores as donors, and the microspore fruit, spores and soil auxiliary cells in the soil are usually collected by wet sieve method. There are many disadvantages in using a single spore as a source of ...

AI Technical Summary

Problems solved by technology

There are many unfavorable factors in using a single spore as a bacterial source. First, the spore infection rate is moderate, and the number of vegetative species accumulates slowly; Easy separation of spores; at the same time larger or smaller particles may be lost during wet sieving

Although pure species can be obtained by using spores as propagules, it is difficult to maximize the role of bacterial agents produced by a single bacterial source in nature, due to changes in the field environment, antagonism between strains, and the selection of strains by host plants Sexuality, etc. will affect a single bacterial source, and the bacterial agent produced thereby is difficult to popularize and apply in the field

Arbuscular mycorrhizal fungi are obligate biotrophic parasites. They can reproduce into spores and sporocarps only after forming mycorrhizae on living plant roots. It is difficult to replicate the rhizosphere microenvironment between mycorrhizae and host plants in the laboratory. The number of root propagules in the soil, survival time, activity, and infection rate vary greatly, and it is difficult to screen out mycorrhizal flora suitable for wild growth indoors.