Preparation method of siganus guttatus chromosome

A technology for spotting basketfish and chromosomes, which is applied in the field of preparation of basketfish chromosomes, which can solve the problems of inconspicuous difference in chromosome shape and large number of chromosomes, and achieve the effect of moderate length, clear centromere and complete number
CN102766692AInactive Publication Date: 2012-11-07EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2012-11-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparation method of a siganus guttatus chromosome. The preparation method comprises the following steps of: (1) taking out head-kidney tissues from siganus guttatus, placing in a culture dish containing physiological saline, cleaning, removing blood cots and other tissues, cutting the head-kidney tissues into pieces, and then fully homogenizing the head-kidney tissues to prepare a cell suspension; (2) performing hypotonicity and fixation; (3) performing fixed centrifugation, and then drilling on a slide; and (4) dyeing, drying the slide, then placing the slide under a microscope for observation, and selecting metaphase cells with good dispersion of the chromosome for performing photomicrometrology. The preparation method disclosed by the invention is simple and convenient in operation and stable in result, expensive reagents and special instruments are not required, and the metaphase mitosis in the siganus guttatus chromosome, which has the advantages of complete number, good dispersion, appropriate length, clear centromeres, appropriate separation of two monomers and clear shape, can be obtained by making the slide.
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Description

technical field

[0001] The invention belongs to the field of preparation of fish chromosomes, and in particular relates to a method for preparing chromosomes of fish-spotted fish. Background technique

[0002] Basketfish (Siganus guttatus), belonging to the class Osteichthyes, subclass Actinopterygii, order Perciformes, suborder Acanthuroidei, family Siganidae, basket fish The genus is an omnivorous, euryhaline, warm-water fish that is distributed from coral reefs to estuary waters. Basket fish is delicious, with high economic and nutritional value. It is popular among consumers in Guangdong, Hong Kong, Southeast Asia, the Middle East, Fiji and other countries and regions.

[0003] Chromosomal karyotype analysis is one of the important contents of fish cytogenetics research. It is not only of great significance for understanding the taxonomic system, evolutionary relationship and chromosome evolution process of fish, but also provides a cytogenetic basis for fish genetic br...

Claims

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