Application of KIT signal related gene QPCR (quantitative polymerase chain reaction) chip to mouse piebaldism detection

A signal-related, mottled disease technology, applied in the biological field, can solve the problems of high cost, emphasis on high-throughput, lack of, etc., to achieve the effect of low cost, simple operation and data analysis
CN102776272AInactive Publication Date: 2012-11-14HANGZHOU NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HANGZHOU NORMAL UNIVERSITY
Publication Date
2012-11-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the biological technical field and relates to an application of a KIT signal related gene QPCR (quantitative polymerase chain reaction) chip to the mouse piebaldism detection. The application of the KIT signal related gene QPCR chip to the mouse piebaldism detection is characterized in that the chip uses genes including Akt1, Bcl2, Cbl, Ccnd3, Ccnd3-ps, Csf1, Crk, Ecm1, Fes, Fyn, Grb7, Hras1, Jak1, Kitl, Lyn, Map2k, Matk, Myb, Nr0b2, Ntrk1, Pdgfa, Pik3ca, Ppp1r13b, Prkcc, Raf1, Sh2b2, Shc1, Snai2, Soat1, Stat1 and Zhx2 as detection sites, Actb is used as the internal reference, and the QPCR chip is used for QPCR amplification in the mouse piebaldism detection. Compared with the gene chip, the KIT signal related gene QPCR chip provided by the invention has the following advantages that 1, the cost is low; 2, the related gene is designed by aiming at a certain signal path or a certain tissue, and a certain specificity is realized; and 3, the operation and the data analysis are relatively simple.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to the application of a QPCR chip for detection of KIT signal-related genes in the detection of mottle disease in mice. Background technique

[0002] The human KIT proto-oncogene originated from HZ4 feline sarcoma virus, located on human chromosome 4q12, in the region around the centrosome of the long arm of chromosome 4, and is an allele of the white spot dominant gene. In mice, the gene is located at about 42 cM from the centromere on chromosome 5. The full length of human KIT genomic DNA is about 89kb, including 21 exons. The protein encoded by the KIT gene is called KIT or c-kit receptor, which is a type I transmembrane glycoprotein with a molecular weight of 145kD. It belongs to the type III protein tyrosine kinase receptor superfamily member and is distributed on the cell membrane surface. Its structure is similar to macrophage colony-stimulating factor and platelet-derived growth...

Claims

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