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Reagent kit for distinguishing and detecting dengue virus/yellow fever virus/west nile virus/chikungunya virus

A technology for chikungunya virus and West Nile virus, which can be used in the determination/inspection of microorganisms, fluorescence/phosphorescence, resistance to vector-borne diseases, etc., and can solve the problems of easy contamination, low sensitivity, and immune cross-reaction cycle Achieve the effect of reducing pollution, high throughput and easy operation

Active Publication Date: 2014-04-23
DAAN GENE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has the disadvantages of low sensitivity, immune cross-reaction, and long cycle; while conventional PCR also has the disadvantage of easy contamination

Method used

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  • Reagent kit for distinguishing and detecting dengue virus/yellow fever virus/west nile virus/chikungunya virus
  • Reagent kit for distinguishing and detecting dengue virus/yellow fever virus/west nile virus/chikungunya virus
  • Reagent kit for distinguishing and detecting dengue virus/yellow fever virus/west nile virus/chikungunya virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Dengue virus / yellow fever virus / West Nile virus / Chikungunya virus identification and detection kit and its use Tube, RT-PCR reaction solution (250μl / tube), RT-PCR enzyme system (75μl / tube) 1 tube, positive quality control (200μl / tube) 1 tube, negative quality control (200μl / tube) 1 tube , DEPC H 2 O (2000μl / tube) 1 tube.

[0031] 2. Collection, preservation and transportation of specimens

[0032] 2.1 Specimen types: serum, mosquito samples.

[0033] 2.2 Specimen collection:

[0034] 2.2.1 Serum: Aseptically collect 3-5ml of venous blood within 5 days after the onset of the disease, let it stand at room temperature for 30 minutes to solidify, then centrifuge at 1500-2000rpm for 10min to remove fibrin and red blood cells, and collect serum in 2ml of sterile screw-top plastic tube.

[0035] 2.2.2 Mosquitoes: collect mosquito samples according to actual work needs. Put the collected mosquitoes directly into the -20°C refrigerator to freeze to death, take th...

Embodiment 2

[0047] Example 2 Application of dengue virus / yellow fever virus / West Nile virus / chikungunya virus differential detection kit to detect clinical samples

[0048] The positive serum samples of dengue virus type I, dengue virus type II, dengue virus type III, dengue virus type IV, yellow fever virus, West Nile virus, and chikungunya virus were selected and identified by the virus culture method. One case, and one case of serum samples each positive for Japanese encephalitis virus, fever with thrombocytopenia syndrome bunya virus, Hantaan virus, and Seoul virus identified by virus culture methods were used as specific samples, and all samples were tested for nucleic acid. The steps of extraction, PCR amplification and result analysis were carried out with reference to Example 1, and the detection of negative and positive quality control products was carried out simultaneously.

[0049] Test result: the amplification curve of the negative quality control product is not S-shaped (se...

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Abstract

The invention relates to a reagent kit for distinguishing and detecting four entomoplily transmitted pathogens, in particular to a reagent kit for distinguishing and detecting dengue virus, yellow fever virus, west nile virus and chikungunya virus by utilizing multiple real-time fluorescent polymerase chain reaction technologies. The reagent kit mainly comprises reverse transcription-polymerase chain reaction (RT-PCR) reaction liquid, primer probe mixed liquid, an RT-PCR reaction enzyme system, diethylpyrocarbonate (DEPC) H2O and packing boxes for separating and packing reagent bottles or tubes. Due to the fact the reagent kit can accurately distinguish the dengue virus, the yellow fever virus, the west nile virus and the chikungunya virus, the reagent kit can be widely applied to the multiple fields of differential diagnosis, inspection & quarantine, prevention and control of epidemic situation and the like of entomoplily transmitted virus.

Description

technical field [0001] The present invention relates to a kind of reagent kit for the identification and detection of four kinds of insect-borne pathogens, in particular to a detection kit for dengue virus, yellow fever virus, West Nile virus and Chikungun using multiple real-time fluorescent polymerase chain reaction technology. Ya virus kit. This kit mainly includes RT-PCR reaction solution, primer-probe mixture, RT-PCR reaction enzyme system, DEPC H 2 O, and the box that separates and centrally packs these reagent bottles or tubes. Since this kit can accurately distinguish dengue virus, yellow fever virus, West Nile virus and chikungunya virus, it can be widely used in differential diagnosis, inspection and quarantine, epidemic prevention and control, etc. multiple fields. Background technique [0002] Arbovirus refers to the virus transmitted by blood-sucking insects. Its characteristic is that the virus can reproduce in the insect body, but the insect itself does not...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
CPCY02A50/30
Inventor 李明郑夔高秀洁陈华云丁国允黄吉城李小波师永霞
Owner DAAN GENE CO LTD
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