Preparation method of yak milk casein antihypertensive peptide

A technology of milk casein and blood pressure-lowering peptide, which is applied in the biological field, can solve the problems of limited application, no antihypertensive effect, strong hydrophobicity, etc., and achieves the effects of high food safety, reduced production cost and simple operation process.

Active Publication Date: 2012-11-21
甘肃华羚生物技术研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are a variety of chemically synthesized peptide drugs as ACE inhibitors, but these synthetic antihypertensive drugs do not last long, blood pressure tends to rebound, and drugs are easily excreted by the kidneys, causing renal function damage
The ACE inhibitory peptide produced from food protein through enzymatic hydrolysis has the advantages of high food safety and no side effects, especially its antihypertensive effect on hypertensive patients, but has no antihypertensive effect on normal blood pressure people.
[0005] The patent No. ZL02139559.4 is authorized to announce a process for preparing casein hypotensive peptides from crude cheese. The hydrolyzate contains more miscellaneous peptides and a large a

Method used

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  • Preparation method of yak milk casein antihypertensive peptide
  • Preparation method of yak milk casein antihypertensive peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Dissolving yak casein with water: Weigh 30g of yak casein, add 500mL of water, adjust the reaction temperature to 37°C, pH 8.0, and stir to dissolve the yak casein; the yak casein was purchased from Hualing Casein Co., Ltd. Ltd., food grade;

[0035] (2) Add trypsin for hydrolysis, the amount of trypsin added is 1.5g, maintain the reaction temperature at 30°C, pH8.0, and hydrolyze for 2 hours; the trypsin is purchased from Beijing Geyuan Tianrun Biotechnology Co., Ltd., and the enzyme activity is 1 :2500;

[0036] (3) Inactivate trypsin, raise the temperature to 90°C and maintain for 30 minutes;

[0037] (4) Precipitate casein: adjust the pH to 4.6 with 5mol / L food grade hydrochloric acid to precipitate casein at its isoelectric point;

[0038] (5) Centrifuge to remove sediment: 4000g centrifugal force, centrifuge for 5-30 minutes, remove the casein precipitate, and keep the supernatant;

[0039] (6) Ultrafiltration: Take the supernatant after centrifugation, pas...

Embodiment 2

[0052] (1) Dissolution of yak casein: Weigh 40g of yak casein, add 500mL of water, adjust the reaction temperature to 40°C, pH 8.0, and stir to dissolve the yak casein; the yak casein was purchased from Hualing Casein Co., Ltd. company, food grade;

[0053] (2) Add trypsin for hydrolysis, the amount of trypsin added is 1.2g, maintain the reaction temperature at 40°C, pH8.0, and hydrolyze for 5 hours; the trypsin is purchased from Beijing Geyuan Tianrun Biotechnology Co., Ltd., and the enzyme activity is 1 :2500;

[0054] (3) Inactivate trypsin, raise the temperature to 90°C and maintain for 30 minutes;

[0055] (4) Precipitate casein: adjust the pH to 4.1 with 5mol / L food grade hydrochloric acid to precipitate casein at its isoelectric point;

[0056] (5) Centrifugation: 4000g centrifugal force, centrifuge for 5-30 minutes, remove the casein precipitate, and keep the supernatant;

[0057] (6) Ultrafiltration: Take the supernatant after centrifugation, pass it through an ult...

Embodiment 3

[0062] (1) Dissolution of yak casein: Weigh 20g of yak casein, add 500mL of water, adjust the reaction temperature to 30°C, pH 8.0, and stir to dissolve the yak casein; the yak casein was purchased from Hualing Casein Co., Ltd. company, food grade;

[0063] (2) Add trypsin for hydrolysis, purchased from Beijing Geyuan Tianrun Biotechnology Co., Ltd., add 1.2 g of trypsin, maintain the reaction temperature at 30 ° C, pH 8.0, and hydrolyze for 4 hours; the trypsin was purchased from Beijing Geyuan Tianrun Biotechnology Co., Ltd. Yuantianrun Biotechnology Co., Ltd., the enzyme activity is 1:2500;

[0064] (3) Inactivate trypsin, raise the temperature to 95°C and maintain for 30 minutes;

[0065] (4) Precipitate casein: adjust the pH to 4.1 with 5mol / L food grade hydrochloric acid to precipitate casein at its isoelectric point;

[0066] (5) Centrifugation: 12000g centrifugal force, centrifuge for 5-30 minutes, remove the casein precipitate, and keep the supernatant;

[0067] (6...

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Abstract

The invention relates to a preparation method of yak milk casein antihypertensive peptide. The method comprises main technical schemes as follows: (1) dissolving yak milk casein; (2) adding trypsin for hydrolysis; (3) inactivating the trypsin; (4) precipitating casein; (5) centrifuging and removing the precipitant; (6) performing ultra-filtration; (7) performing nano-filtration; and (8) performing spray drying. Raw materials for the method provided by the invention are unique; the process is scientific and reasonable, simple and feasible; the product is the casein antihypertensive peptide with rather high purity with the ACE (angiotensin converting enzyme) inhibition activity higher than 70%; the product is safe and free from toxic side effects, has nutritional and assistant medical treatment functions, and can be used as an additive widely applied in the fields of food, healthcare products and medicines and the like.

Description

technical field [0001] The invention uses trypsin to hydrolyze yak milk casein to prepare hypotensive peptides, belongs to the field of biotechnology, and in particular relates to membrane separation technology of polypeptides. Background technique [0002] A large number of studies have found that protein is not completely absorbed by the human body in the form of amino acids, but more in the form of small molecule peptides. These small molecular peptides composed of several amino acids not only have nutritional functions and physiological activities, but also have certain functional properties, such as anti-oxidation, antibacterial properties, and many new functions such as improving immunity. [0003] Antihypertensive peptide is such a small molecule peptide with the function of lowering blood pressure. The molecular weight is mainly concentrated around 1500u. It is an inhibitor of angiotensin converting enzyme (Angiotensin-converting Enzyme, ACE), so it is also called AC...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61P9/12
Inventor 谢小冬宋礼纪银莉高维东何潇葛静微苏艳萍
Owner 甘肃华羚生物技术研究中心
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