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Alfalfa growthpromoting rhizobacteria MJM-11 and application thereof

A technology of MJM-11 and bacterial strains, applied in the field of microorganisms, can solve problems such as improving salt tolerance

Active Publication Date: 2013-11-13
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Alfalfa is the most salt-tolerant leguminous herbage in the world, but its salt-tolerance still needs to be improved in actual cultivation in medium-to-severe saline-alkali land

Method used

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  • Alfalfa growthpromoting rhizobacteria MJM-11 and application thereof
  • Alfalfa growthpromoting rhizobacteria MJM-11 and application thereof
  • Alfalfa growthpromoting rhizobacteria MJM-11 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1, screening and identification of alfalfa rhizosphere growth-promoting bacteria

[0060] 1. Screening

[0061] (1) Take 1g of rhizosphere soil sample in 50mL of PAF medium, shake culture at 28°C. The PAF medium contains peptone, casein hydrolyzate, MgSO 4 , K 2 HPO 4 ,glycerin.

[0062] (2) Take 1 mL of shaken PAF culture medium, place it in 50 mL of PAF medium, and culture with shaking at 28°C.

[0063] (3) Take 1mL of the obtained PAF culture solution, place it in 50mL of DF salt culture solution, and incubate with shaking at 28°C.

[0064] The DF salt broth contains KH 2 PO 4 , Na 2 HPO 4 , MgSO 4 , FeSO 4 , glucose, gluconic acid, citric acid, (NH 4 ) 2 SO 4 , H 3 BO 3 , MnSO 4 , ZnSO 4 , CuSO 4 , MoO 3 .

[0065] (4) Take 1 mL of the obtained DF salt culture solution, and add it to 50 mL without (NH 4 ) 2 SO 4 , but containing 3mM 1-aminocyclopropane-1-carboxylic acid (ACC) in the above-mentioned DF salt culture solution, culture...

Embodiment 2

[0075] Embodiment 2, the application of strain MJM-11 (Enterobacter ludwigii) in the preparation of ACC deaminase

[0076] 1. Preparation of ACC deaminase

[0077] TSB medium: tryptone 17g, soytone 3g, NaCl 5g, glucose 2.5g, K 2 HPO 4 Dissolve 2.5g in 1000ml of distilled water and adjust the pH value to pH=7.5.

[0078] ADF medium: KH 2 PO 4 4.0g, Na 2 HPO 4 6.0g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.1g, glucose 2.0g, gluconic acid 2.0g, citric acid 2.0g, trace elements 0.1mL, add in distilled water to dissolve, adjust the pH value to pH=7.5, set the volume to 1000ml, add 1-amino Cyclopropane-1-carboxylic acid (ACC), so that the final concentration is 3.0mmol / L (you can first prepare the mother liquor 0.5mol / L); among them, trace elements (100mL): H 3 BO 3 10mg, MnSO 4 11.2mg, ZnSO 4 124.6mg, CuSO 4 78.2mg, MoO 3 10 mg.

[0079] None (NH 4 ) 2 SO 4 DF medium: KH 2 PO 4 4.0g, Na 2 HPO4 6.0g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.1g, glucose 2.0g, glu...

Embodiment 3

[0087] Embodiment 3, the application of bacterial strain MJM-11 (Enterobacter ludwigii) in preparing IAA synthesis

[0088] 1. Preparation of IAA

[0089] DF medium: KH 2 PO 4 4.0g, Na 2 HPO 4 6.0g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O 0.1g, glucose 2.0g, gluconic acid 2.0g, citric acid 2.0g, (NH 4 ) 2 SO 4 2.0g, 0.1mL of trace elements, dissolved in distilled water, adjust the pH value to pH=7.5, and dilute to 1000ml. The preparation of trace elements is the same as above.

[0090] The test strain MJM-11 (Enterobacter ludwigii) was cultured in DF medium at 28°C and 180rpm for 2 days, and then transferred to different concentrations (0, 50, 100, 200, 500 μg L-Trp·mL -1 ) tryptophan (L-Trp) in fresh DF medium, cultured at 28° C., 180 rpm for 2 days, and collected the fermentation product. Sampling to measure the OD of the bacterial solution 600 value, and then put the rest of the fermentation product (bacteria liquid) at room temperature (25°C) at 8000rpm for 10min...

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Abstract

The invention discloses an alfalfa growth-promoting rhizobacteria MJM-11 and application thereof. An Enterobacter ludwigii strain MJM-11 has a preservation number of CGMCC No.6295. The Enterobacter ludwigii strain MJM-11 can be applied in at least one of the following four ways: 1, preparation of an ACC (1-aminocyclo propane-1-carboxylate) ammonialyase; 2, preparation of indoleacetic acid; 3, preparation of siderophores; and 4, promotion of the growth of plants under the saline-alkali stress. Experiments prove that the alfalfa growth-promoting rhizobacteria MJM-11 which is obtained through separation can be used for synthesizing the ACC ammonialyase and the IAA (Indole Acetic Acid) siderophores and is capable of effectively promoting the alimentation of plants in a saline-alkali stress environment, regulating the growth of plants and enhancing the stress resistance capacity of plants in adverse conditions.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an alfalfa rhizosphere growth-promoting bacterium MJM-11 and its application. Background technique [0002] Alfalfa is a perennial leguminous plant, rich in various essential amino acids, vitamins and minerals, and as the main grass species in animal husbandry, it has been widely planted all over the world. Its cultivation history has been more than 2,000 years, and it has outstanding advantages In terms of feeding: high grass yield, long service life, strong regeneration, mowing resistance, good grass quality, strong palatability, rich nutrition, increasing production in fertile fields, maintaining water and soil, etc. It is a multifunctional forage grass, known as The reputation of "King of Grass". [0003] Alfalfa is the most salt-tolerant legume herbage in the world, but its salt-tolerance still needs to be improved in the actual cultivation of medium-severe saline-al...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/88C12P17/10C12P1/04A01N63/00A01P21/00A01C1/00A01G7/06C12R1/01
Inventor 郭长虹马嘉敏康慧颖蔡洪生谢宝明
Owner HARBIN NORMAL UNIVERSITY
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