Method for integrally marking drosophila proteomes by using stable isotope labeling with amino acids in cell culture (SILAC) and special culture medium

A technology of proteome and culture medium, applied in application, animal feed, animal feed, etc., can solve the problems affecting the accuracy of quantification and the sensitivity of detection
CN102805052AActive Publication Date: 2012-12-05INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
Publication Date
2012-12-05

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Abstract

The invention discloses a method for integrally marking drosophila proteomes by using stable isotope labeling with amino acids in cell culture (SILAC) and a special culture medium. The special culture medium for marking drosophila by using the SILAC is prepared according to the following method: a saccharomycete body marked by using the SILAC is coated on a drosophila culture medium to obtain the special culture medium for marking the drosophila by using the SILAC. An experiment proves that the culture medium for marking the drosophila by using the SILAC can be specially used for marking the drosophila proteomes by using the SILAC, can achieve rapid and high-efficient marking, and creates conditions for preparation of quantified interior labels and high-precision quantified proteome studying.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method and a special culture medium for using SILAC to integrally mark the fruit fly proteome. Background technique

[0002] Fruit flies (Drosophila melanogaster) are tiny insects. Since Morgan began using it to study genetics, Drosophila has gradually become a model organism for the study of genetics, physiology, development and evolutionary biology. Drosophila has many advantages such as fast growth, easy breeding and reproduction, easy maintenance of large groups, low price, rich phenotypes, and most of the signaling pathways are consistent with higher organisms, making Drosophila become the gold bug in modern biological research. .

[0003] Proteins are direct performers of biological functions. Proteomics is an emerging discipline that systematically identifies and quantifies intracellular proteins and studies their biological functions. With the completion of Drosophila g...

Claims

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