Method for extracting solanesol by activating waste and defective tobacco by enzyme catalysis

A catalytic activation and tobacco extraction technology, applied in the separation/purification of hydroxyl compounds, can solve the problems of reducing the economic value of solanesol products, shortening the service life of production equipment, affecting the market price of solanesol, and achieving biological activity The effects of complete preservation, environmental protection, and reduction of production safety hazards

Inactive Publication Date: 2012-12-12
王星敏
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main shortcoming of this method is: (1) the product that this method produces is solanesol crude extract and thick nicotine hydrochloride solution, and product purity is low, directly affects the commercial price of solanesol, reduces the economical value of solanesol product value
(2) the method adopts 0.5~4% sodium hydroxide solution to process tobacco, uses sodium hydroxide in the production process, causes environmental pollution, increases environmental management cost, shortens the service life of production equipment, increases

Method used

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  • Method for extracting solanesol by activating waste and defective tobacco by enzyme catalysis
  • Method for extracting solanesol by activating waste and defective tobacco by enzyme catalysis
  • Method for extracting solanesol by activating waste and defective tobacco by enzyme catalysis

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Experimental program
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Example Embodiment

[0023] Example 1

[0024] The specific steps of an enzyme-catalyzed activation method for extracting solanesol from waste tobacco are as follows:

[0025] (1) Raw material pretreatment

[0026] Wash 40g of discarded tobacco leaves, dry them, place them in an oven, bake them in an oven at 100°C for 60 minutes, and take them out. After being crushed by a pulverizer, they are sieved through a 60 mesh molecular sieve to obtain tobacco leaves with a particle size of less than 60 mesh. Pack in bags for spare.

[0027] (2) Enzymatic hydrolysis reaction

[0028] After step (1) is completed, the ratio of mass (g) of solid tobacco waste treated in step (1): mass of enzyme (g): volume of water (ml) is 1:0.4:5. In the tobacco solid waste pretreated in step (1), add enzymes and water, stir evenly, then adjust the pH of the system to 6 with sulfuric acid solution, and then place the pH-adjusted mixture in In a shaker, perform enzymatic hydrolysis and activation for 8 hours at a temperature of 40°C...

Example Embodiment

[0035] Example 2

[0036] An enzyme-catalyzed activation method for extracting solanesol from waste tobacco is the same as in Example 1, wherein:

[0037] In step (1), the oven temperature is 110°C, the drying time is 30 minutes, and the discarded tobacco leaves are passed through a 160-mesh sieve.

[0038] In step (2), the ratio of mass of tobacco solid waste (g): mass of enzyme (g): volume (ml) of water is 1:0.6:15, and the pH of the system is adjusted to 9 with sodium hydroxide solution. , Under the condition that the temperature of the water bath on the shaker is 60 ℃, the enzymatic hydrolysis is activated for 2 hours. The enzyme is a composite enzyme with a mass ratio of ligninase:cellulase of 1:6.

[0039] In step (3), the ratio of mass (g) of tobacco solid waste pretreated in step (1): volume (ml) of composite extractant solution is 1:12, and the volume ratio of composite extractant ethanol: methanol is 1:9. The ultrasonic power is 40W, the ultrasonic temperature is 55°C, th...

Example Embodiment

[0042] Example 3

[0043] An enzyme-catalyzed activation method for extracting solanesol from waste tobacco is the same as in Example 1, wherein:

[0044] In step (1), the oven temperature is 85°C, the drying time is 90 min, and the discarded tobacco leaves are passed through a 20-mesh sieve.

[0045] In step (2), the ratio of mass of tobacco solid waste (g): mass of enzyme (g): volume (ml) of water is 1:0.1:1, and the pH of the system is adjusted to 4 with sulfuric acid solution. The temperature of the water bath on the shaker was 25°C, and the enzymatic hydrolysis was activated for 14 hours. The enzyme is a composite enzyme with a mass ratio of ligninase:cellulase of 1:1.

[0046] In step (3), the ratio of mass (g) of the tobacco solid waste pretreated in step (1): volume (ml) of the composite extractant solution is 1:8, and the volume ratio of the composite extractant ethanol: methanol is 1:4. The ultrasonic power is 200W, the ultrasonic temperature is 30°C, the ultrasonic time ...

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Abstract

The invention relates to a method for extracting solanesol by activating waste and defective tobacco by enzyme catalysis, belonging to the technical field of solanesol extraction. The simple technique comprises the following steps: pretreating the raw material tobacco solid waste, carrying out enzymolysis reaction and ultrasonic treatment, preparing solanesol, and preparing the subsidiary product nicotine, thereby obtaining the product. The method provided by the invention has the characteristics of wide raw material sources, low energy consumption in the production process, low corrosion to production equipment, low organic solvent consumption and the like, sufficiently utilizes the waste, and is beneficial to environmental protection; and the prepared solanesol product has the advantages of high extraction rate, high purity and complete biological activity. The invention can be widely used for extracting solanesol, nicotine and other natural products from tobacco solid waste, thereby recycling and reutilizing waste and defective tobacco.

Description

technical field [0001] The invention belongs to the technical field of solanesol extraction, and in particular relates to a method for extracting solanesol from solid tobacco waste by enzyme catalysis and activation. Background technique [0002] Solanesol is a three-sesquiterpene alcohol, which is mainly stored in tobacco leaves (0.3% to 3% of the total weight), potato leaves (about 1.5%), mulberry leaves (about 0.1%), etc. As an important raw material for drug synthesis, solanesol is mainly manifested in the following aspects: ① synthesis of ubiquinone compounds such as coenzyme Q10; ② synthesis of drugs for the treatment of tumors; ③ synthesis of anti-ulcer drugs; ④ synthesis of drugs for the treatment of cardiovascular and cerebrovascular diseases; Anti-HIV drugs. Various synthetic drugs derived from solanesol as synthetic precursors are not only widely used in human medicine, but also have broad application prospects in the growing veterinary drug industry for livestoc...

Claims

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Application Information

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IPC IPC(8): C07C33/02C07C29/74
Inventor 王星敏
Owner 王星敏
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