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Method for extracting solanesol by activating waste and defective tobacco by enzyme catalysis

A catalytic activation and tobacco extraction technology, applied in the separation/purification of hydroxyl compounds, can solve the problems of reducing the economic value of solanesol products, shortening the service life of production equipment, affecting the market price of solanesol, and achieving biological activity The effects of complete preservation, environmental protection, and reduction of production safety hazards

Inactive Publication Date: 2012-12-12
王星敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main shortcoming of this method is: (1) the product that this method produces is solanesol crude extract and thick nicotine hydrochloride solution, and product purity is low, directly affects the commercial price of solanesol, reduces the economical value of solanesol product value
(2) the method adopts 0.5~4% sodium hydroxide solution to process tobacco, uses sodium hydroxide in the production process, causes environmental pollution, increases environmental management cost, shortens the service life of production equipment, increases production cost; (3) the method The method uses 2-12% hydrochloric acid to extract nicotine, and hydrochloric acid is used in the production process, which further increases the cost of environmental treatment, and at the same time aggravates the corrosion of production equipment, further increasing the production cost; 1:0.6~1:2 n-hexane or No. 6 solvent oil phase, the use of organic solvents in the production process increases, which increases the potential safety hazards in the production process and further increases production costs

Method used

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  • Method for extracting solanesol by activating waste and defective tobacco by enzyme catalysis
  • Method for extracting solanesol by activating waste and defective tobacco by enzyme catalysis
  • Method for extracting solanesol by activating waste and defective tobacco by enzyme catalysis

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The specific steps of a method for enzymatically activating waste tobacco to extract solanesol are as follows:

[0025] (1) Raw material pretreatment

[0026] Wash 40g of discarded tobacco leaves, dry them, place them in an oven, bake them in an oven at 100°C for 60 minutes, take them out, crush them with a pulverizer, and pass through a 60-mesh molecular sieve to obtain tobacco leaves with a particle size of less than 60 mesh. Pack in separate bags and set aside.

[0027] (2) Enzymolysis reaction

[0028]After the first (1) step is completed, first according to the quality (g) of the tobacco solid waste after the (1) step treatment: the quality (g) of enzyme: the ratio of the volume (ml) of water is 1: 0.4: 5 Ratio, in the tobacco solid waste after the pretreatment in step (1), add enzyme and water, after stirring evenly, then adjust the pH value of the system with sulfuric acid solution to be 6, then place the mixed solution after adjusting the pH value in In a sha...

Embodiment 2

[0036] A method for enzymatically activating waste tobacco to extract solanesol, the same as in Example 1, wherein:

[0037] In step (1), the temperature of the oven is 110° C., the drying time is 30 minutes, and the waste tobacco leaves are passed through a 160-mesh sieve.

[0038] In the (2) step, the quality (g) of tobacco solid waste: the quality (g) of enzyme: the volume (ml) ratio of water is 1: 0.6: 15, and the pH value of regulating system with sodium hydroxide solution is 9 , under the condition that the temperature of the water bath on the shaker is 60° C., the enzymatic activation is carried out for 2 hours. The enzyme is a composite enzyme with a mass ratio of ligninase:cellulase of 1:6.

[0039] In the (3) step, the quality (g) of the tobacco solid waste after the pretreatment of the (1) step: composite extractant solution volume (ml) ratio is 1: 12, and composite extractant ethanol: the volume ratio of methyl alcohol is 1:9. The ultrasonic power is 40W, the ul...

Embodiment 3

[0043] A method for enzymatically activating waste tobacco to extract solanesol, the same as in Example 1, wherein:

[0044] In step (1), the oven temperature is 85° C., the drying time is 90 minutes, and the waste tobacco leaves are passed through a 20-mesh sieve.

[0045] In the (2) step, the quality (g) of tobacco solid waste: the quality (g) of enzyme: the volume (ml) ratio of water is 1: 0.1: 1, and the pH value of regulating system with sulfuric acid solution is 4, in The temperature of the water bath on the shaker was 25° C., and the enzymatic activation was carried out for 14 hours. The enzyme is a composite enzyme with a mass ratio of ligninase:cellulase of 1:1.

[0046] In the 3rd step, the quality (g) of the tobacco solid waste after the pretreatment of the 1st step: composite extractant solution volume (ml) ratio is 1: 8, and composite extractant ethanol: the volume ratio of methyl alcohol is 1:4. The ultrasonic power is 200W, the ultrasonic temperature is 30°C,...

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Abstract

The invention relates to a method for extracting solanesol by activating waste and defective tobacco by enzyme catalysis, belonging to the technical field of solanesol extraction. The simple technique comprises the following steps: pretreating the raw material tobacco solid waste, carrying out enzymolysis reaction and ultrasonic treatment, preparing solanesol, and preparing the subsidiary product nicotine, thereby obtaining the product. The method provided by the invention has the characteristics of wide raw material sources, low energy consumption in the production process, low corrosion to production equipment, low organic solvent consumption and the like, sufficiently utilizes the waste, and is beneficial to environmental protection; and the prepared solanesol product has the advantages of high extraction rate, high purity and complete biological activity. The invention can be widely used for extracting solanesol, nicotine and other natural products from tobacco solid waste, thereby recycling and reutilizing waste and defective tobacco.

Description

technical field [0001] The invention belongs to the technical field of solanesol extraction, and in particular relates to a method for extracting solanesol from solid tobacco waste by enzyme catalysis and activation. Background technique [0002] Solanesol is a three-sesquiterpene alcohol, which is mainly stored in tobacco leaves (0.3% to 3% of the total weight), potato leaves (about 1.5%), mulberry leaves (about 0.1%), etc. As an important raw material for drug synthesis, solanesol is mainly manifested in the following aspects: ① synthesis of ubiquinone compounds such as coenzyme Q10; ② synthesis of drugs for the treatment of tumors; ③ synthesis of anti-ulcer drugs; ④ synthesis of drugs for the treatment of cardiovascular and cerebrovascular diseases; Anti-HIV drugs. Various synthetic drugs derived from solanesol as synthetic precursors are not only widely used in human medicine, but also have broad application prospects in the growing veterinary drug industry for livestoc...

Claims

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Application Information

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IPC IPC(8): C07C33/02C07C29/74
Inventor 王星敏
Owner 王星敏
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