Unlock instant, AI-driven research and patent intelligence for your innovation.

Broad spectrum antibacterial compound medicine as well as application of artesunate and azithromycin in preparation of antibacterial compound medicine

A technology of azithromycin and artesunate, which is applied in the direction of antibacterial drugs, resistance to vector-borne diseases, and pharmaceutical formulations. It can solve the problems of inability to broad-spectrum antibacterial, many adverse reactions, and inconvenient use, so as to achieve fewer adverse reactions and better medication. The effect of small dosage and convenient use

Inactive Publication Date: 2013-01-02
GUILIN PHARMA
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There have been antibacterial studies on artesunate, and it was found that artesunate combined with gentamicin, salamicillin, cefpiramide and other antibiotics can significantly enhance the antibacterial effect of antibiotics on Escherichia coli (Li Bin, Zhou Hong "Progress in the Study of the Pharmacological Effects of Artemisinin and Its Derivatives", Chinese Clinical Pharmacology and Therapeutics, 2010.5, 572-576), patent document CN 101020056A (Patent No. ZL 200710078254.5) discloses artesunate and gentamicin There is a certain antibacterial synergistic effect, but the clinical dose of gentamicin is similar to the poisoning dose, with many adverse reactions, high toxicity, difficult absorption after oral administration, and in addition, it cannot be broad-spectrum antibacterial; in addition, artesunate and cefpiramide, stimulant The combined application of ampicillin, ampicillin and gatifloxacin can only be used for injection and cannot be taken orally, which is inconvenient to use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Broad spectrum antibacterial compound medicine as well as application of artesunate and azithromycin in preparation of antibacterial compound medicine
  • Broad spectrum antibacterial compound medicine as well as application of artesunate and azithromycin in preparation of antibacterial compound medicine
  • Broad spectrum antibacterial compound medicine as well as application of artesunate and azithromycin in preparation of antibacterial compound medicine

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0029] Experimental example 1, in vitro detection of the antibacterial effect of artesunate and azithromycin combined application on gram-positive bacteria and gram-negative bacteria

[0030] 1 material

[0031] 1.1 Experimental drugs

[0032] Artesunate (Guilin South Pharmaceutical Co., Ltd.), azithromycin (Henan Tianfang Pharmaceutical Co., Ltd.).

[0033] 1.2 Test bacteria

[0034] Gram-positive bacteria: Staphylococcus aureus, Enterococcus.

[0035] Gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa.

[0036] 1.3 Reagents

[0037]MHA medium (source: Beijing Land Bridge Technology Co., Ltd.), MHA broth (source: Beijing Land Bridge Technology Co., Ltd.), nutrient agar medium (source: Guangdong Huankai Microbiology Technology Co., Ltd.), sodium chloride (source: Shantou City, Guangdong Xilong Chemical Factory), sterilized water.

[0038] 1.4 Instruments

[0039] Micro injection gun, incubator, 12×8 96-well plate.

[0040] 2 methods...

experiment example 2

[0081] Experimental example 2. In vivo detection of the antibacterial effect of artesunate and azithromycin combined application on Gram-negative bacteria

[0082] Experiment 2.1 The protective effect of combined application of artesunate and azithromycin on mice challenged with Gram-negative bacteria

[0083] 1. The protective effect of combined application of artesunate and azithromycin on mice challenged with Gram-negative bacteria

[0084] In this experiment, the protective effect of artesunate combined with azithromycin on mice challenged by Gram-negative bacteria (Escherichia coli as an example) was studied. Two methods of administration were carried out: intramuscular injection and oral administration respectively. The specific experimental methods are as follows:

[0085] 140 mice, weighing about 20g, half male and half male, were randomly divided into seven groups according to injection and oral administration, with 10 mice in each group, divided into control group, E...

experiment example 3

[0100] Experimental example 3. In vivo detection of the antibacterial effect of artesunate and azithromycin combined application on Gram-positive bacteria

[0101] Experiment 3.1. The protective effect of combined application of artesunate and azithromycin on mice challenged with Gram-positive bacteria

[0102] 1. The protective effect of combined application of artesunate and azithromycin on mice challenged with Gram-positive bacteria

[0103] The operation was the same as experiment 2.1, and the experiment was carried out by replacing Escherichia coli with Staphylococcus aureus, and the experimental results are shown in Table 11 and Table 12.

[0104] The experimental results of Table 11 and Table 12 show that artesunate and azithromycin are both effective for intramuscular injection and oral administration of Staphylococcus aureus in the combined application of artesunate and azithromycin. OK, but oral is also effective without increasing the dose.

[0105] Table 11 In vi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a broad spectrum antibacterial compound medicine as well as a new application of artesunate and azithromycin in preparation of antibacterial compound medicine. The active components of the broad spectrum antibacterial compound medicine at least comprise artesunate and azithromycin mixed according to the mass ratio of 1 to (1-4). Experiments prove that the compound of artesunate and azithromycin is significantly enhances the antibacterial efficacy on gram negative bacteria and gram positive bacteria through injection or oral administration, reduces the use dose of the antibacterial medicine, as well as significantly reduces the death rate of bacteria including gram negative bacteria and gram positive bacteria on mice. The antibacterial compound medicine provided by the invention are subjected to combined application, and has the characteristics of being few in administration dose, easy to absorb, wide and strong in antibacterial spectrum, little in adverse reaction, and convenient to use (oral administration is available), and being suitable for popularization and application.

Description

technical field [0001] The invention belongs to the field of combined application of medicines, in particular to an antibacterial compound medicine obtained by combining artesunate and azithromycin. Background technique [0002] Infectious diseases caused by pathogenic microorganisms and parasites are a class of common diseases. When using chemical drugs to treat such diseases, we should pay attention to the relationship between the body, pathogens and drugs. On the one hand, we should use antibacterial drugs rationally to exert their antibacterial effects, and at the same time, we should mobilize the body's disease resistance to inhibit pathogenic microorganisms. The growth of the body can quickly restore the body to health; on the other hand, it should avoid and reduce the adverse reactions of drugs on the body and the emergence of bacterial resistance. [0003] Artesunate (artesunate) is a derivative of artemisinin (artemisinin), its chemical name is dihydroartemisinin-1...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7052A61P31/04A61K31/357
CPCY02A50/30
Inventor 郑清四刘臣玉陆艳
Owner GUILIN PHARMA