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Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptide by hydrolyzing rice protein isolate with complex enzyme

A technology for compound enzyme hydrolysis and protein separation, applied in the field of functional food processing, can solve the problems of low peptide yield and ACE inhibitory activity, high ash content, and achieve the effect of strong activity, low ash content, and shortened hydrolysis time.

Inactive Publication Date: 2013-01-09
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods mostly use a single protease to hydrolyze protein, ultrafiltration separation, activated carbon decolorization and refining, etc., the peptide yield and ACE inhibitory activity are low, and the ash content is high, which affects its application.

Method used

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  • Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptide by hydrolyzing rice protein isolate with complex enzyme
  • Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptide by hydrolyzing rice protein isolate with complex enzyme
  • Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptide by hydrolyzing rice protein isolate with complex enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Alkaline protease (Alcalase 2.4L FG, 2.4AU / g, produced by Bacillus subtilis, whose activity was determined by kinetic diformaldehyde casein method.) and trypsin (Trypsin Novo 6.0, 1250USP / mg, produced by Danish Novozymes Company) were adopted. Extracted from porcine pancreas, and its activity was determined by ultraviolet spectrophotometry.) The preparation process conditions are: the mass volume ratio concentration of the rice protein isolate aqueous solution is 7.5%, the pH value is 9, the reaction temperature is 50°C, and the enzyme amount is 54AU / kg , after 120 min of hydrolysis reaction, quickly adjust the reaction temperature to 37°C, pH value to 8, and add trypsin hydrolysis reaction for 30 min according to the amount of enzyme added at 20 USP / kg. Then adjust the pH to 5.0 to inactivate enzymes and remove macromolecular peptides. The enzymatic solution was centrifuged at 4000 r / min for 20 min, and the supernatant was separated through an ultrafiltration membrane ...

Embodiment 2

[0025] Alkaline protease (Alcalase 2.4L FG, 2.4AU / g, produced by Bacillus subtilis, whose activity was determined by kinetic diformaldehyde casein method) and trypsin (Trypsin Novo 6.0, 1250 USP / mg, It is extracted from porcine pancreas, and its activity is determined by ultraviolet spectrophotometry.) The preparation process conditions are: the mass volume ratio concentration of rice protein isolate aqueous solution is 7.5%, the pH value is 9, the reaction temperature is 50°C, and the enzyme amount is 60 AU / kg, after 120 min of hydrolysis reaction, quickly adjust the reaction temperature to 37°C, pH value to 7.5, and add trypsin hydrolysis reaction for 30 min according to the amount of enzyme added at 25 USP / kg. After the reaction, adjust the pH value to 5.0, inactivate the enzyme and remove the macromolecular polypeptide. The enzymatic solution was centrifuged at 4000 r / min for 20 min, and the supernatant was separated through an ultrafiltration membrane with a molecular we...

Embodiment 3

[0029] Alkaline protease (Alcalase 2.4L FG, 2.4AU / g, produced by Bacillus subtilis, whose activity was determined by kinetic diformaldehyde casein method.) and trypsin (Trypsin Novo 6.0, 1250USP / mg, produced by Danish Novozymes Company) were adopted. Extracted from porcine pancreas, and its activity was determined by ultraviolet spectrophotometry.) The preparation process conditions are: the mass volume ratio concentration of rice protein isolate aqueous solution is 7.5%, the pH value is 9, the reaction temperature is 50°C, and the enzyme amount is 48 AU / kg, after 120 min of hydrolysis reaction, quickly adjust the reaction temperature to 37°C, pH value to 7.5, and add trypsin hydrolysis reaction for 30 min according to the amount of enzyme added at 12.5USP / kg. Then adjust the pH to 5.0 to inactivate enzymes and remove macromolecular peptides. The enzymatic solution was centrifuged at 4000 r / min for 20 min, the supernatant was separated through an ultrafiltration membrane with...

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Abstract

The invention discloses a method for preparing an ACE (Angiotensin Converting Enzyme) inhibitory peptide by hydrolyzing rice protein isolate with a complex enzyme. The method comprises the following steps of: (1) adjusting temperature of rice protein isolate aqueous solution with mass-volume concentration of 5-8 percent to 45-55 DEG C and pH (Potential of Hydrogen) value to 8.5-9.5, and adding alkaline protein enzyme according to an enzyme adding amount of 48-60 AU / kg to hydrolyze for 120-150 min; (2) adjusting system temperature to 36-38 DEG C and pH value to 7-8 and adding trypsin according to an enzyme adding amount of 12.5-25 USP / kg to hydrolyze for 30-60 min; and (3) adjusting the pH value to 5.0-5.5 and performing solid-liquid separation, ultrafiltration membrane screening, desalting, concentration and freeze drying on enzymatic hydrolysate in sequence to prepare the ACE inhibitory peptide product. According to the method, two enzymes act on different enzyme cutting sites respectively; the prepared ACE inhibitory peptide has high yield and strong activity; and the method has the advantages of simple process, mild reaction condition and contribution to industrial production.

Description

technical field [0001] The invention belongs to the technical field of food processing, and specifically relates to functional food processing technology, in particular to a method for preparing ACE (ie, angiotensin-converting enzyme) inhibitory peptide by hydrolyzing rice protein isolate with complex enzymes. Background technique [0002] About 12 million people die from high blood pressure every year in the world. In developed countries and relatively developed countries, the prevalence rate is as high as 20%. In developing countries, the prevalence rate of high blood pressure is also increasing in recent years. At present, the prevalence of hypertension in my country has risen to 11.88%, and the number of patients is estimated to be more than 100 million. More than 95% of the hypertensive population has essential hypertension, and the inhibition of ACE activity can effectively reduce essential hypertension. Commonly used ACE inhibitors in clinical practice include Captop...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 陈季旺蔡广霞吴永宁
Owner WUHAN POLYTECHNIC UNIVERSITY
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