Preparation method of xenograft bone graft substitute material
A technology of bone material and bone transplantation, applied in medical science, prosthesis, etc., can solve the problems of carcinogenicity, teratogenicity, and low fat removal efficiency, and achieve low antigenicity, good osteogenesis ability, and low cost Effect
Active Publication Date: 2014-10-29
胡懿郃 +4
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[0005] In addition, the most commonly used degreasing method for xenograft bone is to use a mixture of methanol and chloroform with a volume ratio of 1:1 for degreasing, but this degreasing method is not efficient in removing fat, and the remaining fat will have antigenicity , and both methanol and chloroform have neurotoxicity, nephrotoxicity, hepatotoxicity, carcinogenicity and teratogenicity, etc., and are toxic solvents. Therefore, the existing degreasing technology for xenograft bone also needs further improvement
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[0075] A preparation method of the bovine cancellous bone graft substitute material of the present invention specifically includes the following steps.
[0076] 1. Take material:
[0077] Under clean conditions, take the fresh bovine tibia upper cancellous bone that has passed the inspection (Brazil virus detection) and slaughtered within 6 hours. The bone knife cleans the surrounding soft tissue, periosteum and bone marrow, and the low-speed saw blade milling cutter processes it into 5mm×5mm×30mm Bone strips or 5mm×5mm×5mm bone granules, rinse the blood on the surface repeatedly with warm water at 40℃~50℃, and drain. They were manually selected without blood stains and no cortical bone components, clean packaged, and refrigerated in a refrigerator at -20°C.
[0078] 2. Degreasing treatment:
[0079] Take the fresh beef cancellous bone strip after the above pretreatment and wrap it with filter paper. Put the filter paper cartridge containing bovine cancellous bone strips into the ex...
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The invention discloses a method of derosination of xenogenic bone material. Fresh xenogenic bone strips are put into an extraction barrel of a Soxhlet extractor, and is conducted to continuous backflow extraction through light petroleum under certain conditions till the color of changed light petroleum conducted to derosination still keeps clear, and then the xenogenic bone material conducted to derosination is obtained. The invention also discloses a method of deproteinization of the xenogenic bone material. The xenogenic bone material conducted to derosination is soaked in derosination treating fluid containing pepsase, the pH value is controlled to be 1.5 to 3 during soaking, alkali liquor is used for performing inactivation to the pepsase, the xenogenic bone material is repeatedly flushed and drained, and then the xenogenic bone material conducted to deproteinization is obtained. The invention also discloses a preparation method of substitute material for the xenogenic bone material. The xenogenic bone material conducted to derosination and deproteinization is conducted to freeze drying and germicidal treatment, and then the substitute material for the xenogenic bone material is obtained. The method is simple for processing and manufacture, the cost is low, and products obtained have low antigenicity and good osteoconduction and biomechanical property.
Description
Technical field [0001] The invention relates to a method for processing bone material and a method for preparing a bone graft substitute material, in particular to a method for processing a xenogeneic bone material and a method for preparing a xenogeneic bone graft substitute material. Background technique [0002] In the clinical work of orthopedic surgery, other reasons such as trauma, tumor, inflammation, and developmental abnormalities often lead to bone defects, causing loss of limb function, and affecting people's quality of life. Bone transplantation is the most commonly used method for the treatment of bone defects. Among them, traditional autologous bone transplantation is the most effective method for clinical treatment of bone defects. However, autologous bone transplantation is at the expense of healthy tissue, which will cause additional surgical damage to the donor area. Complications such as pain, hematoma, fractures, etc.; and the source of autologous bone and the...
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IPC IPC(8): A61L27/36
Inventor 胡懿郃汪龙周天健周佳林雷鹏飞谢杰
Owner 胡懿郃