Hg2+ sensor based on upconversion NaYF4 resonance energy transfer and detection method of Hg2+ sensor

Abstract

The invention discloses an Hg2+ sensor based on upconversion NaYF4 resonance energy transfer and a detection method of the Hg2+ sensor. The detection method provided by the invention comprises the following steps: an ultrasonic micro-emulsion method is utilized for obtaining the water soluble and monodisperse rare-earth upconversion nano-particles with uniform grain sizes, which are superficially modified with rhodamine thiolactone, namely the Hg2+ sensor based on upconversion NaYF4 resonance energy transfer; in the presence of Hg2+, the rhodamine thiolactone decorated on the surfaces of the nano-particles and Hg2+ are specially combined to be conducted for ring opening, a maximum absorption peak at 555nm stimulated by near-infrared light at 980nm and an emission peak of the NaYF4 nano-particles at 543nm are just overlapped to be conducted for resonance energy transfer, and the fluorescent emission peaks of the NaYF4 nano-particles are quickly quenched, so that the aim of qualitatively and quantitatively detecting Hg2+ can be achieved by detecting the fluorescence intensity. Compared with the traditional detection method, the detection method provided by the invention is small in background interference, strong in signal, low in cost, and has the characteristics of fastness, accuracy, high flexibility and high selectivity.

Description

technical field [0001] The invention belongs to Hg 2+ The technical field of sensor preparation, in particular to a method based on upconversion NaYF 4 Hg for resonance energy transfer 2+ Sensors and their detection methods. technical background [0002] With the development of industrial technology, the problem of heavy metal pollution is becoming more and more serious, especially the problem of mercury pollution. Mercury is highly toxic and extremely harmful to living organisms and the environment. Mercury is volatile. Mercury and its compounds can invade the human body through different channels such as the respiratory tract, skin or digestive tract. The food chain has a strong ability to enrich mercury. The mercury enriched in the human body can cause harm to the nervous system of the brain. And can cause diseases of the heart, liver, stomach and other tissues and organs. Therefore, it is very important for the detection and monitoring of mercury ions in daily life,...

AI Technical Summary

Problems solved by technology

[0003] At present, there are various detection methods for mercury ions, most of which focus on spectroscopic methods, such as atomic absorption spectrometry, atomic emission spectrometry, spectrophotometry and fluorescence analysis methods, etc. Most of the analytical methods have very high requirements for instruments and samples. High, time-consuming, and will be limited in practical applications

And fluorescent molecular probe technology (Himali S., Hewage, Eric V.Anslyn.J.AM.CHEM.SOC.2009,131,13099–13106; Tsui-Lien Kao.J.Org.Chem.2005,70,2912 -2920; Denis Svechkarev.J.Phys.Chem.A 2011,115,4223–4230) to some extent make up for its shortcomings, and can realize the in situ detection of heavy metal mercury ions, but most organic fluorescent probe molecules The water solubility is not good, and its fluorescence lifetime is short, the stability is poor, and there are still some limitations in the detection of actual samples.

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