Method for improving enzymatic hydrolysis efficiency of bamboo reed stalks

A technology of enzymatic hydrolysis efficiency and straw, applied in the field of life engineering, can solve problems such as low efficiency of enzymatic hydrolysis and saccharification, and achieve the effects of improving reducing sugar conversion rate, reducing lignin content and high efficiency

Inactive Publication Date: 2013-01-23
SOUTHWEST UNIVERSITY +1
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AI Technical Summary

Problems solved by technology

[0005] Aiming at the low efficiency of enzymatic hydrolysis and saccharification of Arundos stalks, the purpose o

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Raw material preparation: Wash the reed bamboo stalks, cut them to 4-5cm, and dry them for later use.

[0032] (2) Pretreatment process: adding NaOH with a mass fraction of 1%, H 2 o 2 The volume fraction was 0.5% in the reaction solution, the temperature was 60°C, the solid-liquid ratio (g / mL) was 1:20, and the pretreatment time was 4, 8, 16, 24, and 32 h, respectively.

[0033] (3) After the pretreatment, wash with water until neutral drying, crush to 60 mesh, measure the lignin content in the pretreated Arundis stalks, and calculate the lignin removal rates as 34.11%, 50.00%, and 62.21% respectively , 70.20%, 70.22%.

Embodiment 2

[0035] (1) Raw material preparation: Wash the reed bamboo stalks, cut them to 4-5cm, and dry them for later use.

[0036] (2) Pretreatment process: adding NaOH with a mass fraction of 1%, H 2 o 2 The volume fraction is 0.5% in the reaction solution, the temperature is 60°C, the pretreatment time is 24 h, and the solid-liquid ratio (g / mL) is 1:30, 1:25, 1:20, 1:15, 1:10.

[0037] (3) After the pretreatment, wash with water until neutral drying, crush to 60 mesh, measure the lignin content in the pretreated Arundis stalks, and calculate the lignin removal rates as 31.67%, 54.45%, and 70.21% respectively , 70.47%, 71.36%.

Embodiment 3

[0039] (1) Raw material preparation: Wash the reed bamboo stalks, cut them to 4-5cm, and dry them for later use.

[0040] (2) Pretreatment process: the mass fractions of sodium hydroxide added to Arundis stalks were 0%, 0.25%, 0.5%, 1%, 1.5%, 2%, H 2 o 2 The volume fraction is 0.5% in the reaction solution, the temperature is 60°C, the solid-liquid ratio (g / mL) is 1:20, and the pretreatment time is 24 h.

[0041] (3) After the pretreatment, wash with water until neutral drying, crush to 60 mesh, measure the lignin content in the pretreated Arundis stalks, and calculate the lignin removal rates are 0.41%, 7.08%, 19.11% respectively , 70.21%, 71.03%, 71.98%.

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Abstract

The invention discloses a method for improving enzymatic hydrolysis efficiency of bamboo reed stalks, comprising the steps of washing the bamboo reed stalks, cutting into short sections and drying for standby application, adding the bamboo reed stalks into mild alkali reaction solution to soak for pretreatment, filtering and washing the pretreated bamboo reed stalks to be neutral, drying and grinding for standby application, adding citric acid-sodium citrate buffer solution and cellulase in the grinded bamboo reed stalks to carry out enzymatic hydrolysis on the grinded bamboo reed stalks, carrying out centrifugal separation on the reaction solution after enzymatic hydrolysis, and taking the supernatant as the enzymatic hydrolysis product of the bamboo reed stalks. According to the method, pretreatment is carried out on ligno-cellulose raw material through a chemical method and the lignin is degraded and the crystalline texture of the cellulose is opened to enlarge the contact surface of the enzyme and the substrate, so that the lignin content in the stalks is considerably reduced, the reducing sugar conversion rate of the bamboo reed stalks after the enzymatic hydrolysis is improved and then the enzymatic hydrolysis efficiency is improved.

Description

technical field [0001] The invention relates to the improvement of the processing technology of reed bamboo stalks, in particular to a method for removing lignin to improve the enzymatic hydrolysis efficiency of reed bamboo stalks, which belongs to the technical field of life engineering. [0002] Background technique [0003] Reed bamboo is a commonly used plant for wetland ecological restoration, but with the long-term operation of wetlands, the effective removal of pollutants needs to be achieved by harvesting reed bamboo regularly. Due to the lack of effective resource utilization technology, the harvested reed bamboo stalks cannot be used in time, causing secondary pollution. Arundis straw is mainly composed of cellulose, hemicellulose and lignin, which is a kind of good biomass energy, so it is of great significance to use biotechnology to biotransform Arundis straw. Enzymatic hydrolysis can convert cellulose in straw into reducing sugars, but the encapsulation of ce...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 黄磊高旭刘明李哲郭劲松杜刚鲁言言
Owner SOUTHWEST UNIVERSITY
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