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Rapid breeding method for Caesalpinia cucullata Roxb.

A fast and budding technology, applied in the field of bioengineering, to achieve the effect of simple equipment, less floor space and low production cost

Inactive Publication Date: 2013-01-30
向华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports on the success of Jianxuefei tissue culture rapid propagation at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Material: See Xuefei with axillary buds as explants

[0025] 1. Selection and sterilization of explants: Young tissues such as fresh shoots and stems of Jianxuefei collected from Cili, Hunan, as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes Finally, place it in 75% alcohol for 10-30 seconds, then disinfect it with 0.1% mercuric chloride for 10 minutes, rinse it with sterile water, cut off the discolored part of the explant and set it aside.

[0026] 2. Germination of buds: inoculate the sterilized buds on N6+6-BA1.2mg / L+NAA0.2mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 12 hours, light intensity 1000LX; 6-8 days after inoculation, buds germinate, and leafy seedlings form in about 10 days.

[0027] 3. Cluster bud induction and proliferation culture: cut the seedlings and transfer them to N6+6-BA0.5mg / L+IAA0.4mg / L medium until cluster buds are formed, the pH ...

Embodiment 2

[0031] Material: See Xuefei with axillary buds as explants

[0032]1. Selection and sterilization of explants: Young tissues such as fresh shoots and stems of Jianxuefei collected from Cili, Hunan, as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes Finally, place it in 75% alcohol for 10-30 seconds, then disinfect it with 0.1% mercuric chloride for 10 minutes, rinse it with sterile water, cut off the discolored part of the explant and set it aside.

[0033] 2. Germination of buds: inoculate the sterilized buds on N6+6-BA1.2mg / L+NAA0.3mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 16 hours, light intensity 1000LX; 7-8 days after inoculation, buds germinate, and leafy seedlings form in about 12 days.

[0034] 3. Cluster bud induction and proliferation culture: cut the seedlings and transfer them to N6+6-BA0.5mg / L+IAA0.4mg / L medium until cluster buds are formed, the pH v...

Embodiment 3

[0038] Material: See Xuefei with axillary bud stems as explants

[0039] 1. Selection and sterilization of explants: Young tissues such as the fresh shoots and stems of Jianxuefei collected from Sangzhi, Hunan, as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes Finally, place it in 75% alcohol for 10-30 seconds, then disinfect it with 0.1% mercuric chloride for 10 minutes, rinse it with sterile water, cut off the discolored part of the explant and set it aside.

[0040] 2. Germination of buds: inoculate the sterilized buds on N6+6-BA1.2mg / L+NAA0.2mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 16 hours, light intensity 1000LX; about 6-8 days after inoculation, the buds germinate, and the seedlings with leaves form about 10 days.

[0041] 3. Cluster bud induction and proliferation culture: cut the seedlings and transfer them to N6+6-BA0.5mg / L+IAA0.4mg / L medium until clu...

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Abstract

The invention provides a rapid breeding method for Caesalpinia cucullata Roxb. According to the method, tissue like a stem of Caesalpinia cucullata Roxb. is cultured on an N6 culture medium, and a rhizome with one or two buds is exactly taken as an explant, then disinfected and inoculated into a culture medium consisting of N6, 0.5 mg / L of 2,4-D, 1.4 mg / L of 6-BA and 0.4 mg / L of IBA for culture; the bud germinates in about 6 to 8 days after inoculation, and a young seedling with leaves is formed in about 10 days; the young seedling is cut down and inoculated into a culture medium consisting of N6, 0.1 mg / L of 2,4-D, 1.8 mg / L of 6-BA and 0.4 mg / L of IBA so as to maintain high-speed progressive increase, and a propagation coefficient is more than 5; a culture medium consisting of N6, 0.5 mg / L of 6-BA and 1.5 mg / L of NAA is selected as a rooting medium, a rooting rate reaches more than 90%, and a complete plant is formed in only about 25 days; and a test-tube plantlet is transplanted into sandy soil, attention is paid to water retention and moisture retention, a transplanting survival rate reaches more than 90% in one month after transplanting, and a high frequency stable regeneration system is established. The method provided by the invention has the advantages of fast budding, a high propagation rate, simpleness, low production cost, capacity of batch production and a high application value.

Description

technical field [0001] The invention relates to the field of bioengineering, and relates to plant tissue culture technology, in particular to a tissue culture method of Jianxuefei. Background technique [0002] Plants are a natural treasure house of medicines. People have a long history of using medicinal plants. About 75% of the world's population uses plants as a source of medicine for treating and preventing diseases (Xing Jianmin, 2001). Humans have discovered many drugs with high physiological activity from plants, and the drugs derived from plants account for more than 25% of the total amount of drugs (Zheng Guangzhi, 1987). Our country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in our country and many countries and regions. However, because the traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources, when the amount of collecti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 董爱文于华忠卜晓英
Owner 向华
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