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Production method of active earthworm protein

A technology of earthworm protein and production method, applied in metabolic diseases, blood diseases, extracellular fluid diseases, etc., can solve problems such as low activity, and achieve the effects of simple production process, reduction of energy consumption, and low production cost

Active Publication Date: 2013-02-13
周维官
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention discloses a red earthworm refined protein polypeptide preparation and its preparation method and application. The active ingredients of red earthworm protein polypeptides are separated by using ultrafiltration membrane separation technology with different pore sizes. The main active ingredient is a molecular weight of ten Protein components with less than 10,000 and more than 50,000 have low activity due to their large molecular weight

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The production method of active earthworm protein of the present invention comprises the steps:

[0046] 1. Inducing emesis: Take 5 kg of clean live worms, put them in a container, then add 5 kg of 5% ethanol liquid, soak for 30 minutes, and make them fully spit out the soil and other sundries in their bodies; after stirring, Take out the ground dragon, rinse it with clean water, and set aside;

[0047] 2. Autolysis of Earthworm: Put the live Earthworm that has been induced to vomit into a container filled with warm water at 40°C and soak it to death, then take it out in time and place it in another container, and then add 0.1% sodium bisulfite liquid 5 Kilogram, put it under the environmental condition of 25 ℃ for 48 hours to let it autodissolve, and set aside;

[0048] 3. Refining: putting the above-mentioned solution with residual earthworm corpses into a colloid mill for refining to obtain a slurry;

[0049] 4. Filtration and centrifugation: filter the slurry obta...

Embodiment 2

[0054] The production method of active earthworm protein of the present invention comprises the steps:

[0055] 1. Emetic induction: Take 17 kg of clean living worms, put them in a container, then add 170 kg of 10% ethanol liquid, soak for 5 minutes, and make them fully spit out the soil and other sundries in their bodies; after stirring, Take out the ground dragon, rinse it with clean water, and set aside;

[0056] 2. Earthworm autolysis: put the live earthworm that has been induced to vomit into a container filled with warm water at 50°C and soak it to death, then take it out in time and place it in another container, and then add 5% sodium bisulfite liquid 17 kg, put it under the environmental condition of 35 ℃ for 10 hours to let it autolyze, and set aside;

[0057] 3. Refining: putting the above-mentioned solution with residual earthworm corpses into a colloid mill for refining to obtain a slurry;

[0058] 4. Filtration and centrifugation: filter the slurry prepared in ...

Embodiment 3

[0063] The production method of active earthworm protein of the present invention comprises the steps:

[0064] 1. Inducing emesis: Take 30 kg of clean living Eisenia chinensis, put it in a container, then add 150 kg of 7% ethanol liquid, soak for 16 minutes, and make it fully spit out the soil and other sundries in its body; after stirring, Take out the ground dragon, rinse it with clean water, and set aside;

[0065] 2. Autolysis of Earthworm: Put the living Earthworm that has been induced to vomit into a container filled with warm water at 46°C, soak it to death, then take it out in time and place it in another container, and then add 3% L-cysteine 90 kg of liquid, put it under the environmental condition of 32 ℃ for 21 hours to let it autodissolve, and set aside;

[0066] 3. Refining: putting the above-mentioned solution with residual earthworm corpses into a colloid mill for refining to obtain a slurry;

[0067] 4. Filtration and centrifugation: filter the slurry prepar...

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Abstract

The invention discloses a production method of an active earthworm protein. The production method comprises the steps of: purging live earthworms, self-dissolving the earthworms, grinding, filtering, centrifuging, carrying out membrane separation and drying to obtain the active earthworm protein. The active earthworm protein has the functions of dissolving thrombus, improving systemic circulation, softening blood vessels, stabilizing plaques, establishing collateral circulation, improving blood and oxygen supply of heart and brain, bi-directionally adjusting blood pressure, reducing blood viscosity and the like. The method disclosed by the invention has the advantages of simple process, high extraction efficiency, low production cost and high production purity and can provide safe and excellent raw materials to foods, health foods, medicines and cosmetics.

Description

technical field [0001] The invention relates to a production method for extracting effective active ingredients from animal bodies, in particular to a method for producing active earthworm protein using earthworm as a raw material. Background technique [0002] Earthworm (Latin name: Pheretima), also known as earthworm, is one of the important Chinese medicinal materials in my country. Earthworms are among the 67 kinds of animal medicines recorded in the earliest monograph of Chinese pharmacy "Shen Nong's Materia Medica". Li Shizhen said in "Compendium of Materia Medica" that it has the functions of dredging meridians, activating collaterals, promoting blood circulation and removing blood stasis, and preventing and treating cardiovascular and cerebrovascular diseases. There are more than 40 medicinal effects in the insect department of "Compendium of Materia Medica". [0003] Active ground dragon protein contains various components such as collagenase, plasmin and lumbrokin...

Claims

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Application Information

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IPC IPC(8): A61K35/64A61P3/06A61P3/10A61P7/02A61P9/10A61P9/12A61K35/62
Inventor 周维官
Owner 周维官
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