Production method of active earthworm protein

A technology of earthworm protein and production method, applied in metabolic diseases, blood diseases, extracellular fluid diseases, etc., can solve problems such as low activity, and achieve the effects of simple production process, reduction of energy consumption, and low production cost

Active Publication Date: 2013-02-13
周维官
View PDF4 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention discloses a red earthworm refined protein polypeptide preparation and its preparation method and application. The active ingredients of red earthworm protein polypeptides are separated by using ultrafiltration mem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] Example 1

[0045] The production method of active earthworm protein of the present invention includes the following steps:

[0046] 1. Induce vomiting: Take 5 kilograms of clean live earthworms, place them in a container, add 5 kilograms of 5% ethanol liquid, soak for 30 minutes to fully spit out the dirt and other sundries in the body; after stirring, Take out the earth dragon and rinse it with clean water, then set aside;

[0047] 2. Earthworm autolysis: Put the emetic live earthworm into a container filled with 40℃ warm water to make it lethal, take it out in time and place it in another container, and add 0.1% sodium bisulfite liquid 5 Kilogram, placed in 25 ℃ environmental conditions for 48 hours to let it auto-dissolve, standby;

[0048] 3. Refining: Put the above-mentioned solution with residual earth dragon corpses into a colloid mill to refining to obtain a slurry;

[0049] 4. Filtration and centrifugation: filter the slurry prepared in step (3) with a 60-mesh screen,...

Example Embodiment

[0053] Example 2

[0054] The production method of active earthworm protein of the present invention includes the following steps:

[0055] 1. Induce vomiting: take 17 kilograms of clean live earthworms, place them in a container, then add 170 kilograms of 10% ethanol liquid, soak for 5 minutes to fully spit out the dirt and other sundries in the body; after stirring, Take out the earth dragon and rinse it with clean water, then set aside;

[0056] 2. Earthworm autolysis: Put the emetic live earthworm into a container filled with 50℃ warm water to make it lethal, take it out in time and place it in another container, and add 5% sodium bisulfite liquid 17 Kg, placed in 35℃ environmental conditions for 10 hours to let it autodissolve, standby;

[0057] 3. Refining: Put the above-mentioned solution with residual earth dragon corpses into a colloid mill to refining to obtain a slurry;

[0058] 4. Filtration and centrifugation: filter the slurry prepared in step (3) with a 300-mesh screen,...

Example Embodiment

[0062] Example 3

[0063] The production method of active earthworm protein of the present invention includes the following steps:

[0064] 1. Induce vomiting: Take 30 kilograms of clean live earthworms, put them in a container, then add 150 kilograms of 7% ethanol liquid, soak for 16 minutes to fully spit out the dirt and other sundries in the body; after stirring, Take out the earth dragon and rinse it with clean water, then set aside;

[0065] 2. Earthworm autolysis: Put the emetic-induced live earthworm into a container filled with 46℃ warm water to make it lethal, take it out in time and place it in another container, and add 3% L-cysteine 90 kilograms of liquid, placed in an ambient condition of 32°C for 21 hours to auto-dissolve, for later use;

[0066] 3. Refining: Put the above-mentioned solution with residual earth dragon corpses into a colloid mill to refining to obtain a slurry;

[0067] 4. Filtration and centrifugation: filter the slurry prepared in step (3) with a 250-me...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a production method of an active earthworm protein. The production method comprises the steps of: purging live earthworms, self-dissolving the earthworms, grinding, filtering, centrifuging, carrying out membrane separation and drying to obtain the active earthworm protein. The active earthworm protein has the functions of dissolving thrombus, improving systemic circulation, softening blood vessels, stabilizing plaques, establishing collateral circulation, improving blood and oxygen supply of heart and brain, bi-directionally adjusting blood pressure, reducing blood viscosity and the like. The method disclosed by the invention has the advantages of simple process, high extraction efficiency, low production cost and high production purity and can provide safe and excellent raw materials to foods, health foods, medicines and cosmetics.

Description

technical field [0001] The invention relates to a production method for extracting effective active ingredients from animal bodies, in particular to a method for producing active earthworm protein using earthworm as a raw material. Background technique [0002] Earthworm (Latin name: Pheretima), also known as earthworm, is one of the important Chinese medicinal materials in my country. Earthworms are among the 67 kinds of animal medicines recorded in the earliest monograph of Chinese pharmacy "Shen Nong's Materia Medica". Li Shizhen said in "Compendium of Materia Medica" that it has the functions of dredging meridians, activating collaterals, promoting blood circulation and removing blood stasis, and preventing and treating cardiovascular and cerebrovascular diseases. There are more than 40 medicinal effects in the insect department of "Compendium of Materia Medica". [0003] Active ground dragon protein contains various components such as collagenase, plasmin and lumbrokin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/64A61P3/06A61P3/10A61P7/02A61P9/10A61P9/12A61K35/62
Inventor 周维官
Owner 周维官
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap