Xylo-oligosaccharide preparation method

Abstract

A xylo-oligosaccharide preparation method comprises the following steps: immersing a raw material in hot water, repeatedly washing, drying, grinding, sieving, adding water to prepare an aqueous solution, heating by microwaves, cooling, adding water to prepare a new aqueous solution, carrying out an enzymatic hydrolysis reaction through adding a xylanase, and carrying out filtering separation of the obtained enzymatic hydrolysis solution to obtain a filter residue and a filtrate; and heating the filter residue by the microwaves, cooling, carrying out the enzymatic hydrolysis reaction through adding the filtrate, repeating the microwave heating and the enzymatic hydrolysis at least one time, collecting the filtrate obtained through the last enzymatic hydrolysis to obtain a crude xylo-oligosaccharide solution, adsorbing by active carbon, dissolving out by alcohol, carrying out reduced pressure concentration to remove the alcohol, re-dissolving by water to obtain a xylo-oligosaccharide solution. The method has the advantages of only once addition of the ferment, no use of a strong acidic solution or a strong alkaline solution, no generation of toxic substances with the generation of the final products, and high productivity.

Description

technical field [0001] The present invention relates to a method for preparing xylooligosaccharides, in particular to a method for preparing xylooligosaccharides by directly hydrolyzing xylan of raw material cell walls with enzymes, which does not need to use strong acid or strong alkali solution and will not produce toxic substances, And both high productivity. Background technique [0002] The cell wall parts of plants are mainly divided into three categories, the first category is cellulose (cellulose) accounting for about 35-50%; the second category is hemicellulose (hemicellulose) accounting for about 20-30%, and the rest is wood prime (lignin). The main component of hemicellulose is xylan (xylan), which is a polymer formed by xylose with β-1,4 bonds. Enzymes, xylanase) or other processing procedures, xylan can be degraded into xylooligosaccharides (xylooligosaccharide). Xylooligosaccharides, also known as xylooligosaccharides and xylooligosaccharides, refer to the g...

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Problems solved by technology

[0004] In addition to the use of enzyme hydrolysis in the aforementioned preparation methods, other methods are often accompanied by the generation of furfural (furfural, furfural). Furfural is toxic, and contact with furfural can irritate the skin and respiratory tract, and even cause pulmonary edema. In severe cases, it will lead to loss of consciousness and respiratory failure. However, the enzymatic hydrolysis method needs to extract xylan from the raw material first and then hydrolyze it. At present, there is no relevant technical literature except that Japanese patent 08-103287 mentions that xylooligosaccharides are directly prepared by enzymatic hydrolysis of citrus pomace. Mention that xylooligosaccharides can be prepared directly by enzymatic hydrolysis of non-citrus pomace raw materials

[0005] The product obtained by the above-mentioned acid treatment preparation method is mainly xylose; the product composition of the high-temperature thermal degradation method is quite complicated, and the total ratio of xylobiose and xylotriose with high functionality is far less than half of the total product, not as good as Specific action of enzymatic hydrolysis and high productivity of xylobiose

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