Method for testing and identifying cepaea hortensis through polymerase chain reaction (PCR)
A snail and forest technology, applied in the field of molecular biology detection and identification, can solve problems such as difficult to distinguish, difficult to identify eggs and young snails, little knowledge about the classification of terrestrial molluscs, etc., to achieve good practicability, rapid and reliable detection and Strong identification and practical effect
Active Publication Date: 2013-03-06
INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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The invention discloses a method for testing and identifying cepaea hortensis through polymerase chain reaction (PCR), and the method comprises the following steps that a specific primer is applied to identify the molecular features of cepaea hortensis; a forward primer of the PCR is CN-(P1):5'-ACCTCCTTCCTTTCTACT-3', and a reverse primer is CN-(P2):5'-GTCAACATCTATCCCAAC-3'. The total volume of a PCR system is 25mu.L, including 12.5mu.L of 2*TaqPCRMasterMix, respectively 0.5mu.L of the forward and reverse primers, 2mu.L of a deoxyribonucleic acid (DNA) template, and the rest is sterilizing ddH2O; and PCR procedures are as follows: predegeneration is carried out for 5 minutes at 5DEG C, 50 seconds at 95DEG C, 30 seconds at 52DEG C and 50 seconds at 72DEG C, and the process is repeated for 35 times; and extension is carried out for 10 minutes at 72DEG C, and the reaction is over. According to the method for testing and identifying cepaea hortensis through PCR, the cepaea hortensis can be quickly and accurately tested and identified, a novel technical means is provided for the testing and the identification of the dangerous and harmful creature cepaea hortensis, and important significance in preventing the cepaea hortensis from being spread and diffused is realized.
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Forward primerReverse primer +2
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Property | Measurement | Unit |
Sensitivity | 10.0 | |
tensile | MPa | |
Particle size | Pa | |
strength | 10 |
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