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Method for extracting and separating Chinese yam polysaccharide

A technology of yam polysaccharide and flocculation method, which is applied in the field of food science, can solve problems such as undiscovered extraction of yam polysaccharide, and achieve the effects of reducing the cost of extraction and separation, reducing the dosage, and shortening the extraction period.

Active Publication Date: 2013-03-13
山东天久生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Through the search of the published patent literature, the technology of extracting yam polysaccharide with flocculant has not been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting and separating yam polysaccharide by flocculation method, the steps are:

[0020] (1) Quantitatively weigh the pulverized and dried yam powder, add distilled water according to the mass ratio of material to water of 1:15, reflux in a water bath at 80°C and stir for extraction for 2 hours, turn the evaporator to concentrate, extract twice, and collect the extraction obtained from the two extractions Concentrated liquid for use;

[0021] (2) Because chitosan is insoluble in water and alkaline solution, and also insoluble in sulfuric acid and phosphoric acid, the chitosan flocculant used in the experiment is prepared with acetic acid solution.

[0022] (3) Pipette 5 mL of the concentrated medicinal solution and place it in a conical flask, control the temperature of the water area, and slowly add a certain amount of chitosan solution under the state of electromagnetic stirring; keep the sample water area warm, stir once every half hour for the first...

Embodiment 2

[0026] (1) Quantitatively weigh the yam powder through pulverization and drying, cook with a material-to-liquid ratio of 1:15, and get the supernatant to concentrate;

[0027] (2) ZTCI+l flocculants include four types: type I, type II, type III, and type IV, and pretreatment agents. ZTCI+l type I flocculants were selected for this experiment according to their main properties. The specific treatment processes of the two components are as follows:

[0028] A component, weigh 1g of A component in ZTCI+1, first use a small amount of water to stir it into a paste, then add the required amount of water, continue to stir until it is completely dispersed, swell for 24 hours, filter it with double gauze, and get 1% A component solution;

[0029] For component B, prepare 1% acetic acid solution, weigh 1g of component B in ZTC1+1, add a small amount of 1% acetic acid solution to make a paste, then add the required amount of acetic acid solution, continue stirring until completely disper...

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PUM

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Abstract

The invention relates to a method for extracting and separating Chinese yam polysaccharide. The method comprises the steps of: quantitatively weighing Chinese yam powder, adding distilled water according to a mass ratio of material to water being 1:15, refluxing in a 80 DEG C water bath and stirring and extracting for 2h, concentrating in an evaporimeter, extracting twice, collecting extracting solutions after the second extraction, and concentrating the extracting solutions for later use; preparing flocculant; moving the concentrated extracting solution into a conical flask, controlling the temperature of the water bath, slowly adding the flocculant under an electromagnetic stirring condition, carrying out centrifugal separation and collecting supernate; and determining the transmittance T700nm and absorbance ABS450nm of the supernate with an ultraviolet visible spectrophotometer, adding absolute ethyl alcohol into the supernate, refrigerating in a refrigerator, carrying out alcohol precipitation overnight and carrying out centrifugal separation, drying the obtained solids in a vacuum drying box to constant weight, thereby obtaining Chinese yam polysaccharide. The Chinese yam polysaccharide extracted by adopting flocculation technology not only has high purity and high yield, but also can have a certain decolorizing effect, and the potential influences of hydrogen peroxide decolorization process on the activity of the Chinese yam polysaccharide can be avoided.

Description

technical field [0001] The invention belongs to the technical field of food science and relates to yam polysaccharides, in particular to a method for extracting and separating yam polysaccharides by flocculation. Background technique [0002] The study found that yam polysaccharides had antioxidant effects on the liver, kidney, myocardium and brain tissue of mice with CCl4-induced experimental liver injury in vivo and in vitro. The content of MDA in the brain and serum shows that yam polysaccharides can resist the formation and scavenging of free radicals, and have protective effects on liver, kidney, myocardium, and brain free radical damage. The anti-free radical activity of yam polysaccharides in vitro was observed by using the method of fenton reaction to induce free radicals in vitro. It was also found that the MDA content of yam polysaccharides in liver, kidney, myocardium, and brain tissue was significantly reduced in the protection group compared with the model group...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 王丽霞刘佳琦李彬
Owner 山东天久生物技术有限公司