Fermentation linkage device and realizing method of fermentation linkage
A technology of linkage device and realization method, applied in biochemical cleaning device, biochemical equipment and method, enzymology/microbiology device, etc. Acquisition and analysis of insufficient credibility, affecting production or engineering design decisions and other issues, to achieve the effect of increasing experimental credibility, shortening the research and development cycle, and shortening the research cycle
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Embodiment 1
[0068] 4 parallel 5L fermentation sub-tanks, including 1 50L sourcing yeast tank and electronic monitoring and control system and data analysis system. The sourcing yeast tank is connected with each tank of the fermentation sub-tank through a pipeline to realize the fermentation from the mother tank The liquid is transported to each sub-tank. Each fermentation tank of the fermentation sub-tank and the fermentation tank of the yeast tank can realize the pH, DO, volume, tail carbon concentration, tail oxygen concentration, oxygen uptake rate (OUR), and carbon dioxide during the fermentation process. Online collection and comparative analysis of parameters such as release rate (CER), respiratory quotient (RQ), and live cell concentration.
[0069] Use the present invention to treat Rhodomonas capsulata ( Rhodopseudomonas capsulatus ) Pilot study on the production process of Coenzyme Q10;
[0070] Strain: Rhodomonas capsulata (selected from Zhejiang Xinhecheng Biomedical Co., Ltd.).
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Embodiment 2
[0083] An experimental device consisting of 8 parallel 5L fermentation sub-tanks and 1 500L sourdough tank is used. The combination can be used to investigate the influence of changes in shear stress and key factors in the fermentation process on fermentation metabolism.
[0084] In this example, the optimization study on the fermentation process of rifamycin, the production strain is: Nocardia mediterraneani ( Nocardia mediterranei ), the experimental steps are as follows:
[0085] (1) Slant medium (%): sucrose 3.0, peptone 0.5, KCl 0.05, FeSO 4 0.001, KH 2 PO 4 0.1, MgSO 4 ·7H 2 O 0.05, agar 1.8, pH 7.0, the balance is water. The seeds are streaked and inoculated into a plate equipped with a slant culture medium at 28°C, a culture time of 48 hours, a relative humidity of 30-60%, and a culture period of 12-15 days.
[0086] (2) Seed medium (%): glucose 2.0, soybean meal 1.0, peptone 1.0, KH 2 PO 4 0.01, KNO 3 0.05, the balance is water. The cultivation temperature is 28°C, an...
Embodiment 3
[0094] An experimental device composed of 4 2L fermentation sub-tanks and 1 50L yeast tank. Using this combination, the optimization of pH control strategies at different stages of micro-aerobic and anaerobic fermentation processes such as lactic acid, ethanol, and caproic acid can be investigated. The 50L or larger production tank is cultivated to a specific stage of the culture solution aseptically transferred to a sterile 2L fermentation sub-tank, by adjusting the ratio of air and nitrogen in the intake air, and combining each fermentation sub-tank collected With the fermentation process parameter information of the yeast tank, control the status of the bacteria in each fermentation sub-tank to be consistent with the yeast tank, and then change the different pH control levels in the sub-tanks. After a period of incubation, perform multi-parameter correlation analysis. Obtain the optimal pH control strategy at this stage to guide the production of the yeast tank.
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