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Fermentation linkage device and method for realizing fermentation linkage

A technology of linkage device and realization method, applied in biochemical cleaning device, biochemical equipment and method, enzymology/microbiology device, etc. Acquisition and analysis of insufficient credibility, affecting production or engineering design decisions and other issues, to achieve the effect of increasing experimental credibility, shortening the research and development cycle, and shortening the research cycle

Active Publication Date: 2015-11-25
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even so, the results obtained are always subject to confusion, affecting decisions regarding production or engineering design issues
[0006] To sum up, the fermentation device in the prior art cannot guarantee the consistency of the initial cell physiological state and the consistency of the reactor growth environment, so the reliability of data collection and analysis is not enough
In addition, the existing fermentation optimization cycle is long and the cost is high

Method used

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  • Fermentation linkage device and method for realizing fermentation linkage
  • Fermentation linkage device and method for realizing fermentation linkage
  • Fermentation linkage device and method for realizing fermentation linkage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Four parallel 5L fermentation sub-tanks, including a 50L fermentation yeast tank and electronic monitoring control system and data analysis system, the fermentation yeast tank is connected to each tank of the fermentation sub-tank through pipelines to realize fermentation from the mother tank The liquid is transported to each sub-tank, and each fermenter of the fermentation sub-tank and the fermenter of the fermenting yeast tank can realize pH, DO, volume, tail carbon concentration, tail oxygen concentration, oxygen uptake rate (OUR), carbon dioxide Online collection and comparative analysis of release rate (CER), respiratory quotient (RQ), viable cell concentration and other parameters.

[0068] Utilize the present invention to rhodomonas capsulata ( Rhodopseudomonas capsulatus ) Pilot-scale research on the production process of coenzyme Q10;

[0069] Strains: Rhodomonas capsulatus (select strains from Zhejiang NHU Biopharmaceutical Company).

[0070] The specific s...

Embodiment 2

[0082] The experimental device consisting of 8 parallel 5L fermentation sub-tanks and 1 500L fermenting yeast tank can be used to investigate the influence of shear stress at a specific stage of the fermentation process and changes in the ambient temperature of key factors on fermentation metabolism.

[0083] In this example, the optimization research on the fermentation process of rifamycin, the production strain is: Nocardia mediterranei ( Nocardiamediterranei ), the experimental steps are as follows:

[0084] (1) Incline medium (%): 3.0 sucrose, 0.5 peptone, KCl0.05, FeSO 4 0.001, KH 2 PO 4 0.1, MgSO 4 ·7H 2 O0.05, agar 1.8, pH7.0, the balance is water. Streak inoculation of the seeds on a flat plate with a slant medium, at 28°C, for 48 hours, with a relative humidity of 30-60%, and a culture period of 12-15 days.

[0085] (2) Seed medium (%): glucose 2.0, bean cake powder 1.0, peptone 1.0, KH 2 PO 4 0.01, KNO 3 0.05, the balance is water. The culture temperature...

Embodiment 3

[0093] The experimental device consists of four 2L fermentation sub-tanks and one 50L fermentation yeast tank. This combination can be used to investigate the optimization of pH value control strategies at different stages of microaerobic and anaerobic fermentation processes such as lactic acid, ethanol, and caproic acid. Aseptically transfer the 50L or larger production tank to a specific stage of culture broth into a sterile 2L fermenter sub-tank, by adjusting the ratio of air and nitrogen in the intake, and combining the collected fermenter sub-tank And the fermentation process parameter information of the fermenting yeast tank, control the bacterial state in each fermenting sub-tank to be consistent with that of the fermenting yeast tank, and then change the different pH control levels in the sub-tank, after cultivating for a period of time, perform multi-parameter correlation analysis, The optimal pH control strategy at this stage is obtained to guide the production of yea...

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Abstract

The invention discloses a fermentation linkage device and a realizing method of fermentation linkage. The fermentation linkage device comprises a main fermentation tank, an auxiliary fermentation tank, a monitoring system and an analysis system, wherein the main fermentation tank is connected with the auxiliary fermentation tank through a pipeline, the main fermentation tank and the auxiliary fermentation tank are respectively connected with the monitoring system, and the analysis system is used for integrally analyzing the data measured by the monitoring system. The fermentation linkage device disclosed by the invention is used for optimizing the fermentation process to shorten the development and research period and reduce the optimization cost.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a fermentation linkage device and a method for realizing the fermentation linkage. Background technique [0002] The biological fermentation process is based on the theme of living cells, and the process of producing valuable target products by giving specific culture environment conditions. The optimization of fermentation process conditions plays a very important role in guiding the smooth and effective development of the production process. The current research on fermentation process optimization is mainly carried out by batch fermentation or continuous fermentation test in a specific fermenter, or using several fermenters to conduct batch experiments at the same time, and some fermenters are equipped with multi-parameter acquisition systems , to optimize the fermentation process. Due to the complexity of microbial physiological and metabolic characteristics ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/36C12Q3/00
Inventor 王泽建张剑坤张明张嗣良储炬庄英萍郭美锦黄明志夏建业杭海峰
Owner EAST CHINA UNIV OF SCI & TECH
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