Method for removing inhibition of reclaimed water to enzymolysis of lignocellulose through reducing agent
A technology of lignocellulose and reducing agent is applied in the field of removing the inhibition of enzymatic hydrolysis of lignocellulose by reclaimed water containing iron ions, and can solve the problems of large water consumption, high water quality requirements, and inability to obtain reducing sugars.
Inactive Publication Date: 2015-01-28
SHANDONG UNIV +1
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Problems solved by technology
Although this method realizes the industrialization of cellulose hydrolysis to produce reducing sugar, it consumes a lot of water and has high requirements on water quality. Reclaimed water or raw water after industrial treatment cannot obtain reducing sugar through the above method, and reclaimed water is not involved. Inhibitory effect on enzymatic hydrolysis of lignocellulose
Method used
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Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1～3
[0027] Cellulase enzyme liquid is prepared according to the following method in the embodiment 1~3:
[0028] The cellulase purchased from Ningxia Xiasheng Industrial Group Co., Ltd. is obtained by fermentation of Penicillium decumbens, and the amount of enzyme added during saccharification is 20mg-40mg / g.
Embodiment 4
[0029] The cellulase enzyme solution in Example 4 was obtained by dissolving acid cellulase purchased from Zaozhuang Jienuo Bioenzyme Co., Ltd. in sodium acetate buffer, and centrifuging to remove impurities. The action pH range of the above acid cellulase is 4.5~6.2, the optimum action pH is 4.8; the temperature range is 50~65°C, the optimum action temperature is 60°C.
Embodiment 1
[0030] The reclaimed water in Example 1 is treated water meeting the GB / TT18920-2002 standard, wherein the concentration of iron ions is 3mM.
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The invention relates to a method for removing inhibition of reclaimed water to enzymolysis of lignocellulose through a reducing agent. The method comprises the following steps: (1) dipping the lignocellulose raw material to be degraded in reclaimed water, so as to obtain the pretreated lignocellulose; (2) dipping the pretreated lignocellulose into dithiothreitol solution, flushing by water or buffer liquid, and drying to obtain the lignocellulose raw material; and (3) dipping the lignocellulose raw material into the cellulase liquid, and saccharifying to obtain the hydrolysate. According to the method, the lignocellulose pretreated by reclaimed water is dipped in dithiothreitol (DTT), and dithiothreitol can remove the oxidation of Fe ion to the substrate, and also can remove the influence of the Fe ion to the enzyme activity, so that the amount of used enzymes can be greatly increased, the industrial cost can be reduced, and the basis is provided for hydrolyzing the lignocellulose by utilizing reclaimed water.
Description
technical field [0001] The invention relates to a method for removing the inhibition of reclaimed water on enzymatic lignocellulose by using a reducing agent, in particular to a method for releasing the inhibition of reclaimed water containing iron ions on enzymatic lignocellulose, and belongs to the technical field of cellulose utilization. Background technique [0002] With the increasing depletion of non-renewable petrochemical raw materials and the harm to the environment, especially the global warming caused by the burning of greenhouse gases, more and more people are paying attention. Using renewable resources as raw materials to replace petrochemical raw materials through a process that is harmless to the environment has become a direction that the world is actively exploring. At present, the development of renewable energy using lignocellulosic biomass, such as corn stalks (corn cobs), wheat straw, rice husks, grains, reeds, and wood, has become one of the future dev...
Claims
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IPC IPC(8): C12P19/14
CPCY02P20/582
Inventor 方诩穆子铭夏蕊蕊王明钰李玲肖林程少博覃树林
Owner SHANDONG UNIV