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Blood purifying adsorbent used for removing blood toxin and preparation method

A blood purification and adsorbent technology, applied in the field of biomedicine, can solve problems such as ligand shedding, high production costs, and risks to patient safety, and achieve high chemical and physical stability, stable physical and chemical properties, and good biocompatibility sexual effect

Active Publication Date: 2013-04-10
佛山市博新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the protein A adsorbent also has some drawbacks that limit its widespread use
First of all, protein A, as a protein ligand, has high production costs and brings heavy economic burden to patients; in addition, the stability of protein ligand is not high, which brings inconvenience to the production, transportation, storage and use of adsorbents. Ligand shedding during treatment can also pose a risk to patient safety

Method used

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  • Blood purifying adsorbent used for removing blood toxin and preparation method
  • Blood purifying adsorbent used for removing blood toxin and preparation method
  • Blood purifying adsorbent used for removing blood toxin and preparation method

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preparation example Construction

[0055] Such as figure 1 As shown, the embodiment of the present invention provides a method for preparing a blood purification adsorbent for removing blood toxins, including:

[0056] S101, activating the solid phase support with an activator, the activator being epichlorohydrin, epibromohydrin or N,N' carbonyldiimidazole.

[0057] Preferably, the activator is epichlorohydrin or N,N'carbonyldiimidazole.

[0058] Preferably, the solid phase carrier is cellulose, agarose or chitosan.

[0059] S102, using 2-amino-5-picoline as a ligand, and immobilizing it on the activated solid phase carrier through chemical coupling to obtain a blood purification adsorbent;

[0060] Wherein, the solid-phase carrier material is in the form of porous microspheres, and its gel pore permeability molecular weight is 11,000-5,100,000.

[0061] Preferably, the solid phase carrier material is in the form of porous microspheres, and its gel pore permeability molecular weight is 11,800-5,000,000.

[...

Embodiment 1

[0137] Add 10ml of cellulose spheres to the water until the total volume reaches 34ml, then add 10ml of 1mol / L sodium hydroxide solution and heat to 40°C. Then add 3ml of epichlorohydrin into the system, and stir at 65 degrees for two hours to carry out system reaction. After the reaction, the activated carrier was obtained by washing with a large amount of water. After measurement, the activated substitution degree is 1.6mmol / g.

[0138] Add 10ml of activated carrier to 150mg of 2-amino-5-picoline and 40ml of 75% ethanol solution. At a constant temperature of 45 degrees, the activated carrier and 2-amino-5-picoline were stirred and reacted in 75% ethanol solution for 6 days. After the reaction, it was washed with ethanol and water. The coupling density was measured to be 1.2 mmol / g.

Embodiment 2

[0140] Take 10 mL of cellulose balls, wash them with acetone to remove the water in the gel, and then disperse them in 10 mL of acetone. Weigh 2g of N,N'carbonyldiimidazole and mix it with cellulose balls, place it in a shaking table at 30°C and 150rpm for 1 hour to react; after the reaction, wash with acetone to remove the unreacted N,N'carbonyldiimidazole to obtain Activated cellulose spheres. After measurement, the activated substitution degree is 2.0mmol / g.

[0141] Take 10mL of N,N'carbonyldiimidazole-activated cellulose spheres, and then disperse them in 10mL of acetone, add 2g of 2-amino-5-picoline to it, and place it in a shaker at 30°C and 150rpm for 3h. Wash with acetone and water for injection to remove unreacted substances to obtain cellulose spheres coupled with 2-amino-5-picoline. The coupling density was measured to be 1.4 mmol / g.

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Abstract

The invention discloses a blood purifying adsorbent used for removing blood toxin and a preparation method, which employs 2-amino-5-methylpyridine as a ligand, and the ligand is fixed on a solid phase carrier through chemical coupling; a solid phase carrier material employs a porous microsphere form, and the glue holes permeate the molecular weight of 11,000-5,100,000. The invention discloses a preparation method of the blood purifying adsorbent used for removing blood toxin, which comprises the following steps: activating the solid phase carrier by an activator, the activator is chloropropylene oxide, epibromohydrib or N, N' carbonyl diimidazole; employing 2-amino-5-methylpyridine as the ligand, and fixing on the activated solid phase carrier through chemical coupling to obtain the blood purifying adsorbent. By using the blood purifying adsorbent, good adsorption capability are provided on IgG, IgM type antibodies and an immunization compound and a hydrophobic molecule beta2 microglobulin. The physical and chemical properties are stable, the blood purifying adsorbent has strong acid and alkali resistant and organic solvent resistance, and the cost is low.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a blood purification adsorbent for removing blood toxins and a preparation method thereof. Background technique [0002] Autoimmune diseases are a class of diseases caused by the disorder of the immune system, which recognizes and damages its own normal cells, tissues, and organs. The conditions of autoimmune diseases are diverse, and more than 40 kinds have been identified. The common ones are: thyroid disease, systemic lupus erythematosus, rheumatoid arthritis, systemic sclerosis, etc. Autoimmune diseases have a very high disability or fatality rate, and my country is also an area where such diseases occur frequently. According to epidemiological statistics, there are more than 1 million patients with systemic lupus erythematosus in my country, the most in the world. The incidence of rheumatoid arthritis in my country is as high as 0.35%-0.4%, and the disability rate is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/24B01J20/28B01J20/30A61M1/38
Inventor 黄飞飞孔耀中廖兴华
Owner 佛山市博新生物科技有限公司
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