Preparation method of double-enzyme glucose sensor based on graphene

A glucose sensor and graphene technology, which can be used in instruments, scientific instruments, measuring devices, etc., can solve problems such as interference signals, and achieve the effects of good biocompatibility, simple and easy preparation method, and good biocompatibility

Inactive Publication Date: 2013-04-10
TONGJI UNIV
View PDF2 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under such a high working potential, many substances (such as uric acid, ascorbic acid, etc.) that coexist in actual biological samples are also oxidized on the electrode surface, resulting in the generation of interference signals

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of double-enzyme glucose sensor based on graphene
  • Preparation method of double-enzyme glucose sensor based on graphene
  • Preparation method of double-enzyme glucose sensor based on graphene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0023] (1) Preparation of functionalized graphene: 10 mg of ionic liquid [BMIM]PF 6 Add to 0.5 mg / mL graphite oxide aqueous solution and sonicate for one hour to obtain a uniformly dispersed mixture. Then KOH (10 mg) was added and ultrasonicated for 30 minutes to obtain a transparent and uniform solution. At 60°C, reflux for 24 hours to obtain functionalized graphene. The obtained product was centrifuged, washed with ethanol and water until neutral, and dried in vacuum at 60°C.

[0024] (2) Preparation of Pt nanoparticles: 1wt% H 2 PtCl 6 Aqueous solution (1ml) was added to 100ml of water and heated to boiling point. Add 3ml of 1wt% sodium citrate aqueous solution quickly again, and keep the boiling point temperature for 1 hour.

[0025] (3) Preparation of dual-enzyme glucose sensor:

[0026] a. Pretreatment of glassy carbon electrodes: firstly polish the glassy carbon electrodes (φ=3mm) with metallographic sandpaper, and then use 1.0μm, 0.3μm and 0.05μm Al 2 o 3 The s...

Embodiment 2

[0030] (1) Preparation of functionalized graphene: 10 mg of ionic liquid [BMIM]BF 4 Add to 0.5 mg / mL graphite oxide aqueous solution and sonicate for one hour to obtain a uniformly dispersed mixture. Then KOH (10 mg) was added and ultrasonicated for 30 minutes to obtain a transparent and uniform solution. At 80°C, reflux reaction for 48 hours to obtain functionalized graphene, the obtained product was centrifuged, washed with ethanol and water until neutral, and dried in vacuum at 60°C.

[0031] (2) Preparation of Pt nanoparticles: 1wt% H 2 PtCl 6 Aqueous solution (1ml) was added to 100ml water and heated to boiling point. Add 3ml of 1wt% sodium citrate aqueous solution quickly again, and keep the boiling point temperature for 1 hour.

[0032] (3) Preparation of dual-enzyme glucose sensor:

[0033]a. Pretreatment of the glassy carbon electrode: First, the glassy carbon electrode (φ=3mm) is polished with metallographic sandpaper, and then polished to a mirror surface with...

Embodiment 3

[0036] (1) Preparation of functionalized graphene: 10 mg of ionic liquid 1-octyl-3-methylimidazolium bromide was added to 0.5 mg / mL graphite oxide aqueous solution and sonicated for one hour to obtain a uniformly dispersed mixture. Then KOH (10 mg) was added and ultrasonicated for 30 minutes to obtain a transparent and uniform solution. At 70°C, reflux reaction for 48 hours to obtain functionalized graphene, the obtained product was centrifuged, washed with ethanol and water until neutral, and dried in vacuum at 60°C.

[0037] (2) Preparation of Pt nanoparticles: 1 wt % H 2 PtCl 6 Aqueous solution (1ml) was added to 100ml of water and heated to boiling point. Add 3 ml of 1 wt % sodium citrate aqueous solution quickly and keep the boiling point temperature for 3 hours.

[0038] (3) Preparation of dual-enzyme glucose sensor:

[0039] a. Pretreatment of glassy carbon electrodes: firstly polish the glassy carbon electrodes (φ=3mm) with metallographic sandpaper, and then use 1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of a double-enzyme glucose sensor based on graphene and belongs to the technical field of material preparation. The method comprises the following steps: adopting ionic liquid to functionally modify the dispersibility of graphene, combining the modified graphene with platinum nono-particles to promote electron transfer, using a layer and layer self-assembly method to decorate the graphene onto a glassy carbon electrode, utilizing a biological signal sensitization amplification effect, combining with glucose oxidase and horseradish peroxidase, drying the substances for 24 hours at 4 DEG C, so as to enable the electrode surface to form a layer of uniform composite membrane, and preparing the double-enzyme glucose sensor which is used for quick electrochemical gauging of glucose. The nanocomposite prepared by the preparation method is good in biocompatibility, low in cost, simple and quick, and utilizes the stationarity of bio-enzymes. The biosensor prepared by the preparation method is high in sensitivity, good in stability, wide in linear range, has certain antijamming capability, and can be widely applied to diabetes diagnosis and food technical monitoring.

Description

technical field [0001] The invention relates to a preparation method of a graphene-based dual-enzyme glucose sensor, belonging to the technical field of material preparation. Background technique [0002] The research on biosensors has gone through three stages of development, namely, the electrocatalysis of the first generation of biosensors with oxygen as a relay, the electrocatalysis of the second generation of biosensors based on artificial media, and the direct electrocatalysis of the third generation of biosensors. [0003] To achieve H 2 o 2 Direct oxidation on electrodes often requires higher working potentials (greater than 0.6V vs. SCE). Under such a high working potential, many substances (such as uric acid, ascorbic acid, etc.) that coexist in actual biological samples are also oxidized on the electrode surface, resulting in the generation of interference signals. Constructing a dual-enzyme system glucose biosensor is an effective way to suppress interference....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/30
Inventor 邱军王珊珊
Owner TONGJI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap