Application of entomogenous fungi liquid fermentation mycelium as prawn feed additive

A technology of liquid fermentation and insect-producing fungi, applied in the application, animal feed, animal feed and other directions, can solve the problems of serious breeding diseases, abuse of antibiotics, unbalanced feed nutrition, etc.

Inactive Publication Date: 2013-04-17
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Shrimp farming is a highly intensive farming model. The existing feed has problems such as nutritional imbalance and antibiotic abuse. The breeding diseases are serious, and drug residues lead to quality and safety problems of aquatic products.

Method used

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  • Application of entomogenous fungi liquid fermentation mycelium as prawn feed additive

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Experimental program
Comparison scheme
Effect test

Embodiment １

[0013] Preparation of liquid fermented mycelium dry powder of 3 kinds of entomogenous fungi:

[0014] 1) Preparation of dry powder of Mortierella sinensis liquid fermentation mycelium: Mortierella sinensis ( Hirsutella inensis ) (Cordyceps sinensis ascospore germination and its anamorphic observation, Guizhou Agricultural Sciences, 2003, 31(1):3-5.) After the strain was activated, inoculated in 150 mL liquid medium (recipe: 200 g / L potato Extract juice, glucose 20 g / L, peptone 3 g / L) in 10 bottles of Erlenmeyer flasks (250 mL), cultured on a constant temperature shaker at a temperature of 17 °C and a speed of 170 rpm for 24 days. The mycelium was collected by centrifugation and dried at 55°C to make mycelium dry powder.

[0015] 2) Preparation of dry powder of Paecilomyces gourni liquid fermentation mycelia: Paecilomyces gourni ( Paecilomyces gunnii ) (Study on the yield of crude organic extract of Paecilomyces gourni liquid fermentation liquid, Biotechnology, 2012, 22 (1)...

Embodiment 2

[0018] The culture experiment of Penaeus vannamei was carried out with a filtered circulating water system, and the size of each culture tank was 50×80 cm. Set up 3 experimental groups and a control group, each set up 3 replicates (each aquaculture tank is 1 replicate), put 100 tails of vannamei (grow in the large pond for 30 days, with an average weight of about 0.23g). The three experimental groups were fed with basal feed supplemented with 5% dry powder of mycelia from liquid fermentation of Mortierella sinensis, Paecilomyces gunis and Paecilomyces pumilii (provided by Fujian Dachang Biotechnology Industrial Co., Ltd., the main component is crude protein 41%, crude fat 5%, crude fiber 4%, crude ash 16%), the control group was fed with basic feed. The daily feeding rate is increased by 10%, and the feeding is divided into two times in the morning and afternoon. Use the filter circulating water machine to circulate for 2-4 hours at noon every day to purify the water quality,...

Embodiment 3

[0022] Choose healthy, uniform-sized Penaeus vannamei (growth for 60 days, with an average body weight of about 0.74 g), set up 3 experimental groups and a control group, set up 3 replicates in each group, put 100 shrimps in each group, and raise them in 50×80 cm water tank. The control group was fed with basal feed (provided by Fujian Dachang Biotechnology Industry Co., Ltd.), and the experimental group was fed with 5% liquid fermented mycelia of Mortierella sinensis, Paecilomyces galenis and Paecilomyces pupae in the basal feed respectively. Dry powder. The daily feeding rate increased by 10%, and the feed was divided into two times in the morning and afternoon. Use the filter circulating water machine to circulate for 2-4 hours at noon every day to purify the water quality, and change half of the water in the bucket every week. During the experiment, the water temperature was 24.5-28.9 ℃, and the water was inflated for 24 hours. After feeding for 25 days, the activities ...

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Abstract

The invention relates to an application of an entomogenous fungi liquid fermentation mycelium as a shrimp feed additive, and belongs to the technical field of feed. The entomogenous fungi liquid fermentation mycelium is a Hirsutella sinensis liquid fermentation mycelium, a Paecilomyces gunnii liquid fermentation mycelium, or a Paecilomyces militaris liquid fermentation mycelium. The entomogenous fungi liquid fermentation mycelium is used for improving the growth indexes such as the survival rate, the weight gain rate and the feed utilization rate of white shrimps and increasing immunity.

Description

technical field [0001] The invention relates to the application of liquid fermented mycelia of entomogenous fungi as a feed additive for prawns, and belongs to the technical field of feed. Background technique [0002] Cordyceps, refers to the complex of Ergotaceae fungi growing on the subseats and larval corpses of pupae and larvae of various Lepidoptera insects. More than 400 kinds of Cordyceps have been found, such as Cordyceps sinensis, Cordyceps japonica, Liangshan Cordyceps, Xinjiang Cordyceps Cordyceps, branched Cordyceps, Huoke Cordyceps, Cordyceps militaris, etc. Natural Cordyceps contains a variety of biologically active substances and is rich in cordycepic acid, unsaturated fatty acids, vitamin B12, ergosterol, six-carbon sugar alcohol, alkaloids, etc. According to experimental research, natural Cordyceps has various medicinal effects such as regulating immune system function, anti-tumor effect, anti-fatigue, anti-virus, and regulating central nervous system func...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/00A23K1/16
Inventor 郑永标林岗黄建忠黄杨
Owner FUJIAN NORMAL UNIV
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