Glucan-containing pharmaceutical composition and preparation method thereof
A technology of dextran and composition, which is applied in the field of dextran-containing pharmaceutical composition and its preparation, and can solve the problems of large toxic side effects and cytotoxicity
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[0036] like figure 1 As shown, the preparation method of the above-mentioned glucan-containing pharmaceutical composition comprises the following steps:
[0037] S10, prepare dextran-polyethyleneimine, and the dextran and polyethyleneimine are connected by a urethane bond.
[0038] The preparation method of dextran-polyethyleneimine comprises the following steps:
[0039] Step 1. Dissolve dextran, 4-dimethylaminopyridine and 4-nitrophenyl chloroformate (PNC) in a mass ratio of 1:0.02~0.05:0.2~0.5. In a mixed solution of dimethyl sulfoxide and pyridine, react in an ice bath for 2 to 6 hours, drop the reaction dropwise into a mixed solution of ethanol and ether to form a precipitate, collect the precipitate by centrifugation, wash with ether, and dry in vacuo Dextran-4-nitro-p-phenylchloroformate is obtained.
[0040] The molecular weight of dextran can be 6000-70000. Dextran has the advantages of low price, good biocompatibility, and biodegradability. When the glucan-conta...
Embodiment 1
[0065] Dissolve 0.3 g of dextran with a molecular weight of 6000 in 12 mL of a mixed solution of dimethyl sulfoxide and pyridine with a volume ratio of 1:1, add 11.28 mg of 4-dimethylaminopyridine and 93 mg of 4-nitro-p-phenylchloroformic acid The ester was reacted in an ice bath for 4 hours. After the reaction, the reaction was dropped into a mixed solution of 120 mL of ethanol and ether to form a precipitate. The precipitate was collected by centrifugation, washed with ether, and dried in vacuo to obtain DEX-PNC.
[0066] The DEX-PNC aqueous solution was added dropwise to an aqueous solution of PEI with a molecular weight of 2000, the molar ratio of PEI and dextran was 1:20, and the reaction was carried out under stirring conditions for 24 hours. In the aqueous solution, a dialysis membrane with a molecular weight cutoff of 3500 was used. Dialyzed for 2 days, filtered and freeze-dried to obtain DEX-PEI.
[0067] Dissolve 5 mg of DOX in 1 mL of DMF solution, add cis-aconitic ...
Embodiment 2
[0073] Dissolve 0.3 g of dextran with a molecular weight of 40,000 in a 1:1 volume ratio of 10 mL of a mixed solution of dimethyl sulfoxide and pyridine, add 20.8 mg of 4-dimethylaminopyridine and 112.08 mg of 4-nitro-p-phenyl chloride Formate, reacted in an ice bath for 2 hours. After the reaction, the reaction was dropped into a mixed solution of 200 mL of ethanol and ether to form a precipitate. The precipitate was collected by centrifugation, washed with ether, and dried in vacuo to obtain DEX-PNC.
[0074] The DEX-PNC aqueous solution was added dropwise to an aqueous solution of PEI with a molecular weight of 600, the molar ratio of PEI and dextran was 1:10, and the reaction was carried out under stirring conditions for 12 hours. In the aqueous solution, a dialysis membrane with a molecular weight cut-off of 3500 was used. Dialyzed for 4 days, filtered and freeze-dried to obtain DEX-PEI.
[0075] Dissolve 5 mg of DOX in 1 mL of DMF solution, add cis-aconitic anhydride acc...
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