Fermentation medium for improving output and purity of beta-polymalic acid produced by zymotechnics

A technology of fermentation medium and polymalic acid, which is applied in the field of fermentation medium to improve the yield and purity of β-polymalic acid produced by fermentation, can solve the problems of high cost of raw materials and difficulty in decolorization, and reduce the cost of raw materials and reduce the fermentation rate. The effect of raw material costs

Active Publication Date: 2013-04-17
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The medium replaces the peptone in the semi-synthetic medium through the optimized compounding of amino acids, purines, and pyrimidines, and uses Aureobasidium pullulans to ferment and produce β-polymalic acid, which can effectively solve the high cost of raw materials in the fermentation process and the extraction process. The problem of difficult decolorization in medium and high temperature, significantly improved the yield and purity of β-polymalic acid, and reduced the production cost

Method used

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  • Fermentation medium for improving output and purity of beta-polymalic acid produced by zymotechnics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1 Preparation of culture medium

[0031] (1) Activation medium (g / L): PDA dextrose agar medium (PDA): Potato 200, glucose 20, agar 2, deionized water supplemented to 1L, pH natural, sterilized by high pressure steam at 121°C for 20 minutes;

[0032] (2) Seed medium (g / L): glucose 80, succinic acid 2, potassium carbonate 0.5, KH 2 PO 4 1. MgSO 4 ·7H 2 O 1, heptahydrate and zinc sulfate 0.5, corn steep liquor, CaCO 3 20 (sterilized separately), make up to 1L of deionized water, and sterilize with high-pressure steam at 121°C for 20 minutes.

[0033] 2. Activation of CGMCC3336 strain: the strain was transferred to PDA slant medium, and activated at 25°C for 3-5 days.

[0034] 3. Seed liquid preparation: select well-grown slanted seeds and wash the spores with sterile saline to prepare spores with a concentration of about 10 6 The spore suspension of individual / ml is inoculated in the 500ml Erlenmeyer flask that 100ml liquid seed medium is housed by 10% (v / v) inocul...

Embodiment 2

[0039] The activation of bacterial classification and the preparation of seed solution are the same as in Example 1

[0040] 3. Fermentation tank fermentation:

[0041] (1) Fermentation medium (g / L): sucrose 160, NaNO 3 5. KH 2 PO 3 0.15, KCl 0.5, MnSO 4 0.08, MgSO 4 ·7H 2 O0.45, CaCO 3 16, NH 4 NO 3 13.5, aspartic acid 0.34, leucine 0.87, threonine 0.24, valine 0.34, histidine 0.3, cytosine 0.0015, adenine 0.001, choline 1.0, make up to 1L of deionized water.

[0042] (2) Fermentation conditions were the same as in Example 1. After the fermentation, the content of β-polymalic acid was detected to be 33g / L, and the purity was 83%.

Embodiment 3

[0044] The activation of bacterial classification and the preparation of seed solution are the same as in Example 1

[0045] 3. Fermentation tank fermentation:

[0046] (1) Fermentation medium (g / L): 200 sucrose, NaNO 3 1, KH 2 PO 3 0.07, KCl 0.7, MnSO 4 0.10, MgSO 4 ·7H 2 O 0.3, CaCO 3 24, NH 4 NO 3 15.5, aspartic acid 0.5, leucine 0.42, threonine 0.15, valine 0.1, histidine 0.5, cytosine 0.005, adenine 0.0055, choline 0.15, make up to 1L of deionized water.

[0047] (2) The fermentation conditions were the same as in Example 1. After the fermentation, the content of β-polymalic acid was detected to be 40g / L, and the purity was 90%.

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Abstract

The invention discloses a fermentation medium for improving output and purity of beta-polymalic acid produced by zymotechnics, and belongs to the field of biofermentation engineering. The fermentation medium is compounded by the following components: cane sugar, sodium nitrate, monopotassium phosphate, potassium chloride, manganese sulfate, bitter salt, calcium carbonate, ammonium nitrate, aminosuccinic acid, amidocaproic acid, threonine, amino-isovalerianic acid, histidine, cytosine, adenine and bilineurine. Through the optimized compound of the amino acid, the bilineurine, purine pyrimidine growth factor, peptone in the conventional semi-synthetic culture medium is substituted, a fire-new compound fermentation medium is obtained, difficulty in fading during extracting can be lowered, the content of beta-polymalic acid in zymotic fluid reaches 33-40 g / L, the purity can reach 80-90 percent, and meanwhile a solid practice basis is established for latter metabolomics research.

Description

Technical field: [0001] The invention belongs to the field of biological fermentation engineering, and in particular relates to a fermentation medium for improving the yield and purity of β-polymalic acid produced by fermentation. Background technique: [0002] At present, most synthetic high molecular polymers are petrochemical products, which have had a profound impact on human living environment and resources. With the strengthening of the country's implementation of environmental protection policies and the accumulation of people's knowledge of environmental protection, the traditional production of non-degradable polymers has changed. Therefore, the focus of people's attention is on environmentally friendly biodegradable polymers. The production of these polymers is conducive to saving energy and resources, slowing down the greenhouse effect; it is conducive to the development of ecologically coordinated processes and products. [0003] Polymalic acid (polyhydroxysucci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12R1/645
Inventor 乔长晟郑志达宋玉民李政吴梦溪
Owner 天津北洋百川生物技术有限公司
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