Functionalized suction head and preparation method thereof
A functional and adsorbent technology, applied in the field of sample pretreatment, can solve the problems of limited development, few types of fibrous adsorbents and block adsorbents, and unsuitable powder adsorbents, etc., to achieve good permeability and preparation The effect of simple method
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Embodiment 1
[0027] Preparation and Characterization of Functionalized Tips Supported by Mesoporous Silica
[0028] Use a pipette to pump / push the silicone potting glue to coat the tip of the 200 μL tip with a layer of film; add mesoporous silica powder to immobilize it on the film at the tip of the tip; The iron wire penetrates the tip of the tip of the immobilized mesoporous silica powder to form a channel; it is cured at room temperature for 24 hours, and the powder not immobilized on the adhesive film is washed away with water to obtain a functionalized tip-immobilized mesoporous silica powder. suction head. The as-prepared functionalized tip immobilized on mesoporous silica was characterized by scanning electron microscopy. Such as figure 1 As shown, through a, it can be seen that there is a channel with a diameter of about 300 μm at the tip of the functionalized tip, which matches the diameter of the thin iron wire used in Example 1; through b, it can be seen that the mesoporous si...
Embodiment 2
[0030] Enrichment of Endogenous Peptides with Functionalized Tips Immobilized on Mesoporous Silica
[0031] (1) Functionalized tips loaded with mesoporous silica to enrich bovine serum albumin (BSA) hydrolyzate
[0032] Such as image 3 As shown, connect the functionalized tip with immobilized mesoporous silica to the pipette, pump / push 200 μL of 20 nM BSA hydrolyzate aqueous solution 20 times to make the polypeptide adsorb to the functionalized tip, wash with 200 μL of water and wash with 5 μL containing 0.1 % trifluoroacetic acid (TFA) with 50% (v / v) acetonitrile (ACN) for desorption. Drop 1 μL sample solution and 1 μL matrix solution (2 mg / mL α-cyano-4-hydroxycinnamic acid (CHCA) dissolved in 50% (v / v) ACN containing 0.1% TFA) on the mass spectrometer target plate, and let it dry at room temperature After drying, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF) analysis was performed. Such as Figure 4 As shown, using the short-ra...
Embodiment 3
[0043] Preparation and application of functionalized tip loaded with C18 bonded silica gel
[0044] (1) Preparation of functionalized tips loaded with C18 bonded silica gel
[0045] Use a pipette to pump / push the silicone potting glue to coat the tip of the 200 μL tip with a film; add C18 bonded silica gel powder to immobilize it on the film at the tip of the tip; use a fine iron tube with a diameter of about 300 μm The thread penetrates the tip of the tip of the C18-bonded silica gel powder to form a channel; it is cured at room temperature for 24 hours, and the powder not immobilized on the film is washed away with water to obtain a functionalized tip with C18-bonded silica gel on the tip.
[0046] Taking commercial Zip-Tip C18 as a control, the functionalized tip loaded with C18-bonded silica gel was used to investigate the treatment of samples with low abundance and high salt concentration.
[0047] (2) Commercialized Zip-Tip C18 for sample desalting
[0048] Such as i...
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