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Denitrifying bacterium and application thereof

A technology of denitrifying bacteria and Stenotrophomonas, which is applied in the field of environmental microorganisms, can solve the problems of denitrification effect, low denitrification efficiency, low tolerance of nitrate and nitrite, and achieve tolerance High and good denitrification effect

Active Publication Date: 2013-05-01
浙江至美环境科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Based on the above-mentioned literature and related patent reports, although the denitrifying bacteria reported in the literature and related patents have shown a certain denitrification efficiency, most of these reports are based on laboratory research, and the conclusions drawn under given laboratory conditions , although it has a certain reference value, but the actual denitrification effect in sewage denitrification application needs to be further verified, and most of the reported denitrifying bacteria have low tolerance to nitrate and nitrite or low denitrification efficiency. Advanced problems, subject to certain restrictions in practical application

Method used

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  • Denitrifying bacterium and application thereof
  • Denitrifying bacterium and application thereof
  • Denitrifying bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Isolation and identification of Stenotrophomonas maltophilia CCTCC M 2012455

[0051] (1) Liquid medium

[0052] CH 3 COONa, 2.5g / L; NaNO 3 , 1.2g / L; K 2 HPO 4 , 0.5g / L; MgSO 4 ·7H 2 O, 0.1g / L; trace element solution, 2mL / L; pH adjusted to 7.2-7.6, sterilized at 121°C for 20 minutes;

[0053] Wherein, the trace element solution components are:

[0054] EDTA, 2.06g / L; FeSO 4 ·7H 2 O, 1.54g / L; MnCl 2 4H 2 O, 0.2g / L; ZnSO 4 ·7H 2 O, 0.1g / L; CuSO 4 ·5H 2 O, 0.02g / L; Na 2 MnO 4 , 0.1g / L; CoCl 2 ·6H 2 O, 0.002g / L.

[0055] The solid medium is the agar with 1.5-2% added to the above-mentioned liquid medium components.

[0056] (2) Strain isolation and screening

[0057] The bacterial strain of the present invention is obtained by separating and obtaining from filler biofilm in a continuously operating moving bed biofilm (MBBR) reactor of Jiaxing Sewage Treatment Plant.

[0058] ① Take the sludge samples on the filler biofilm and serially dilute ...

Embodiment 2

[0077] Embodiment 2 The cultivation of Stenotrophomonas maltophilia CCTCC M 2012455

[0078] (1) Liquid medium

[0079] CH 3 COONa, 2.5g / L; NaNO 3 , 1.2g / L; K 2 HPO 4 , 0.5g / L; MgSO 4 ·7H 2 O, 0.1g / L; trace element solution, 2mL / L; pH adjusted to 7.2-7.6, sterilized at 121°C for 20 minutes;

[0080] Wherein, the trace element solution components are:

[0081] EDTA, 2.06g / L; FeSO 4 ·7H 2 O, 1.54g / L; MnCl 2 4H 2 O, 0.2g / L; ZnSO 4 ·7H2 O, 0.1g / L; CuSO 4 ·5H 2 O, 0.02g / L; Na 2 MnO 4 , 0.1g / L; CoCl 2 ·6H 2 O, 0.002g / L.

[0082] The solid medium is agar powder with 1.5% (w / v) added to the above medium components.

[0083] (2) Inoculate the bacterial strain of the present invention stored at -86°C with 50% glycerol onto the above solid medium, and place it in an anaerobic incubator at 33.5°C for upside-down activation for 36 to 48 hours for later use.

[0084] (3) Inoculate the activated strain into a 110mL serum bottle filled with 50mL of liquid medium, and shake...

Embodiment 3

[0087] Example 3 Denitrification ability test of Stenotrophomonas maltophilia CCTCC M 2012455

[0088] NaNO 3 and NaNO 2 As a nitrogen source, the strain growth and denitrification ability were investigated.

[0089] Denitrification medium: CH 3 COONa, 2.5g / L; NaNO 3 / NaNO 2 (according to test requirements); K 2 HPO 4 , 0.5g / L; MgSO 4 ·7H 2 O, 0.1g / L; trace element solution, 2mL / L; sterilized at 121°C for 20 minutes;

[0090] Wherein, the trace element solution components are:

[0091] EDTA, 2.06g / L; FeSO 4 ·7H 2 O, 1.54g / L; MnCl 2 4H 2 O, 0.2g / L; ZnSO 4 ·7H 2 O, 0.1g / L; CuSO 4 ·5H 2 O, 0.02g / L; Na 2 MnO 4 , 0.1g / L; CoCl 2 ·6H 2 O, 0.002g / L.

[0092] In the 110mL serum bottle, add 50mL denitrification culture solution, 2mL embodiment 2 expands the bacterial solution after cultivating, wherein NO 3 - -N and NO 2 - -N concentrations were respectively set to 70mg / L, 140mg / L, 210mg / L, 280mg / L and 350mg / L, the initial pH value was controlled at 7.5, and cu...

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Abstract

The invention discloses a denitrifying bacterium and the application thereof. The denitrifying bacterium is named as Stenotrophomonas maltophilia CM-NRD3, which has be preserved in the China center for type culture collection (CCTCC) in Wuhan university in November 12, 2012, and has the preservation number as CCTCC NO:M2012455. The invention further provides an application of the CCTCC M2012455 to biological denitrificaion in sewage or water. The strain has strong denitrification ability on trate nitrogen and nitrite nitrogen, can remove CODcr in the sewage or the water, is suitable for various nitrogen-polluted water qualities, can also be used for partial nitrification or denitrification process, can be applicable to biological denitrificaion treatment of water with low carbon to nitrogen ratio, and is beneficial to popularization and application.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a denitrifying bacterium and its application. Background technique [0002] In recent years, the discharge of nitrogen-containing industrial sewage and the excessive application of chemical fertilizers in agriculture have caused nitrogen-containing compounds such as nitrate and nitrite to pollute groundwater and surface water, directly or indirectly endangering the quality of drinking water and human health. Water quality deterioration and eutrophication caused by nitrogen-containing sewage entering water bodies are also becoming more and more serious, and nitrogen pollution needs to be solved urgently. [0003] Biological denitrification treatment of nitrogen-containing sewage has become the mainstream denitrification technology due to its advantages of low cost, low energy consumption, good treatment effect, and no secondary pollution. In the past two decades, a num...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
Inventor 谢柳杨岳平杨浩峰周俊利胡安辉张挺
Owner 浙江至美环境科技有限公司
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