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Method for enriching clenbuterol, ractopamine and salbutamol in urine sample of breeding livestock

A technology for ractopamine and albuterol, which is applied in the field of sample pretreatment in analytical chemistry, can solve problems such as workload consumption, and achieve the effects of less adsorbent dosage, high enrichment multiple, and less analyte destruction.

Inactive Publication Date: 2013-05-01
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in the food safety detection and analysis of trace analytes, nearly half of the errors come from the pretreatment of samples, while the errors that really come from instrument detection are less than one-third, and nearly 70% of the workload is spent In the pre-processing stage of the sample

Method used

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  • Method for enriching clenbuterol, ractopamine and salbutamol in urine sample of breeding livestock
  • Method for enriching clenbuterol, ractopamine and salbutamol in urine sample of breeding livestock

Examples

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Embodiment 1

[0028] Example 1 Synthesis of Sulfonated Polystyrene Magnetic Balls with a Diameter of 70 nm

[0029] Referring to the literature (Yan F, Li J, Zhang J, Liu F, Yang W. J. Nanopart. Res., 2009, 11(2):289~296), the chemical co-precipitation method was used to synthesize superparamagnetic Fe 3 o 4 single nanoparticle, and Fe 3 o 4 The surface oil of the nanoparticles was acidified, washed with absolute ethanol and then blown dry with nitrogen; take 5 g of the surface oil acidified Fe 3 o 4 Nanoparticles, suspended Fe in 5 mL of styrene-hexadecane mixture with a volume ratio of 15:1 3 o 4 Nanoparticles, ultrasonic 5~10 min to form a magnetic fluid, transfer the magnetic fluid into 400 mL, 0.01M NaHCO containing 0.5% SDS 3 In the solution, 300 W (25KHz) power continued to phacoemulsify for 30 minutes to form a fine emulsion; add 0.1g K 2 S 2 o 8 After reacting at 70°C for 20-40 min, add 0.5 g of sodium vinyl sulfonate and continue the reaction for 12 h. The magnetic f...

Embodiment 2

[0030] Example 2 Synthesis of sulfonated polystyrene magnetic balls with a diameter of 100 nm

[0031] Referring to the literature (Yan F, Li J, Zhang J, Liu F, Yang W. J. Nanopart. Res., 2009, 11(2):289~296), the chemical co-precipitation method was used to synthesize superparamagnetic Fe 3 o 4 single nanoparticle, and Fe 3 o 4 The surface oil of the nanoparticles was acidified, washed with absolute ethanol and then blown dry with nitrogen; take 5 g of the surface oil acidified Fe 3 o 4 Nanoparticles, suspended Fe in 5 mL of styrene-hexadecane mixture with a volume ratio of 15:1 3 o 4 Nanoparticles, ultrasonic 5~10 min to form a magnetic fluid, transfer the magnetic fluid into 400 mL, 0.01M NaHCO containing 0.4% SDS 3 In the solution, 300 W (25KHz) power continued to phacoemulsify for 30 minutes to form a fine emulsion; add 0.1g K 2 S 2 o 8 After reacting at 70°C for 20-40 min, add 0.5 g of sodium vinyl sulfonate and continue the reaction for 12 h. The magnetic ...

Embodiment 3

[0032] Example 3 Synthesis of Sulfonated Polystyrene Magnetic Balls with a Diameter of 200 nm

[0033] Referring to the literature (Yan F, Li J, Zhang J, Liu F, Yang W. J. Nanopart. Res., 2009, 11(2):289~296), the chemical co-precipitation method was used to synthesize superparamagnetic Fe 3 o 4 single nanoparticle, and Fe 3 o 4 The surface oil of the nanoparticles was acidified, washed with absolute ethanol and then blown dry with nitrogen; take 5 g of the surface oil acidified Fe 3 o 4 Nanoparticles, suspended Fe in 5 mL of styrene-hexadecane mixture with a volume ratio of 15:1 3 o 4 Nanoparticles, ultrasonic 5~10 min to form a magnetic fluid, transfer the magnetic fluid into 400 mL, 0.01M NaHCO containing 0.25% SDS 3 In the solution, 250 W (25KHz) power continued to phacoemulsify for 30 minutes to form a fine emulsion; add 0.1g K 2 S 2 o 8 After reacting at 70°C for 20-40 min, add 0.5 g of sodium vinyl sulfonate and continue the reaction for 12 h. The magneti...

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Abstract

The present invention discloses a method for enriching clenbuterol, ractopamine and salbutamol in a urine sample of breeding livestock, and relates to the field of analytical chemistry, particularly to a sample pretreatment method. The method comprises: carrying out a sample pretreatment, adding sulfonic group surface-modified polystyrene superparamagnetic nanometer beads or sub-micron beads to adsorb, carrying out elution, and collecting the eluent, wherein the elution solution can be directly used for liquid chromatography-mass spectrometry to quantitatively analyze contents of clenbuterol, ractopamine and salbutamol. The method has characteristics of simple operation process, high extraction recovery rate, less elution solvent use amount, and no requirement of rotary evaporation.

Description

technical field [0001] The invention relates to the field of sample pretreatment in analytical chemistry, in particular to a method for enriching clenbuterol, ractopamine and albuterol in urine samples of farmed livestock with sulfonic acid group-modified polystyrene magnetic spheres. Background technique [0002] Clenbuterol, ractopamine, and salbutamol are phenylethanolamine compounds, which belong to β-receptor agonist drugs, which can promote the growth of animals such as cattle, pigs, sheep, poultry, etc., and enhance fat catabolism in the body, significantly improving leanness. meat rate. Therefore, since the 1980s, β-receptor agonists have been added to feed in large quantities to promote the growth of livestock and increase the lean meat rate. [0003] β-receptor agonists tend to accumulate residues in animal tissues, especially internal organs. After entering the human body through the food chain, they can cause muscle tremors, arrhythmia, headache and other sympto...

Claims

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Application Information

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IPC IPC(8): G01N30/08
Inventor 熊勇华许恒毅吴科盛郭亮梁语嫣徐峰赖卫华
Owner NANCHANG UNIV
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