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Preparation method of medical metal surface slow-released growth factor coating

A growth factor, metal surface technology, used in coatings, medical science, prostheses, etc., can solve the problems of inactivation, inability to achieve slow release of growth factors, inactivation of growth factors, etc., and achieve the effect of maintaining activity

Inactive Publication Date: 2013-05-15
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current method of loading growth factors is to directly adsorb the growth factors on the surface of the material, but this method cannot achieve the slow release of the growth factors, and the growth factors are easily inactivated by various factors when exposed to the physiological environment; Combining the treated biomedical metal materials with growth factors in a covalent manner, although this method can realize the immobilization of growth factors, but the covalent bonding method often leads to the inactivation of growth factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of a slow-release growth factor coating on a medical metal surface, the specific steps of which are as follows:

[0022] A. Package of growth factors:

[0023] The growth factor BMP-2, which has a promoting effect on bone growth, is dissolved in a chondroitin sulfate solution with a concentration of 0.5 mg / ml to obtain a chondroitin sulfate solution containing BMP-2, and the concentration of BMP-2 in the chondroitin sulfate solution The concentration is 0.5μg / ml;

[0024] The described chondroitin sulfate solution containing growth factor BMP-2 was mixed with 0.5 mg / ml polylysine solution in a volume ratio of 1:2, stirred, centrifuged, and freeze-dried to obtain Nanoparticles of BMP-2;

[0025] B. Carboxylation of graphene oxide:

[0026] Concentration of 20mg / ml ClCH 2 COONa solution and NaOH 80mg solution with a concentration of 20mg / ml were formulated into a mixed solution at a volume ratio of 1:1, and then 80mg of graphene oxide was added to...

Embodiment 2

[0034] A preparation method of a slow-release growth factor coating on a medical metal surface, the specific steps of which are as follows:

[0035] A. Package of growth factors:

[0036] Dissolving the growth factor VEGF, which can promote bone growth, in a heparin solution with a concentration of 1.5 mg / ml to obtain a heparin solution containing VEGF, and the concentration of VEGF in the heparin solution is 5 μg / ml;

[0037] Mix the heparin solution containing the growth factor VEGF and the chitosan solution of 2.5 mg / ml in a volume ratio of 1:4, stir, centrifuge, and freeze-dry to obtain nanoparticles loaded with BMP-2;

[0038] B. Carboxylation of graphene oxide:

[0039] Concentration of 20mg / ml ClCH 2 The COONa solution and the NaOH80mg solution with a concentration of 20mg / ml are formulated into a mixed solution at a volume ratio of 1:1, and then 80mg graphene oxide is added to the 5ml mixed solution to react for 2h, so that the oxygen-containing groups on the graphen...

Embodiment 3

[0047] A preparation method of a slow-release growth factor coating on a medical metal surface, the specific steps of which are as follows:

[0048] A. Package of growth factors:

[0049] The growth factor TGF-β2, which can promote bone growth, was dissolved in a sodium alginate solution with a concentration of 2.5 mg / ml to obtain a sodium alginate solution containing TGF-β2, and the concentration of TGF-β2 was 10 μg / ml;

[0050] The sodium alginate solution containing the growth factor TGF-β2 is mixed with the chitosan solution of 1.0 mg / ml in a volume ratio of 1:5, stirred, centrifuged, and freeze-dried to obtain BMP-2-loaded nanoparticles;

[0051] B. Carboxylation of graphene oxide:

[0052] Concentration of 20mg / ml ClCH 2 COONa solution and NaOH80mg solution with a concentration of 20mg / ml are prepared into a mixed solution at a volume ratio of 1:1, and then 80mg of graphene oxide is added to 4ml of the mixed solution to react for 1.5h to carboxylate the oxygen-contain...

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PUM

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Abstract

The invention discloses a preparation method of a medical metal surface slow-released growth factor coating. The method comprises the steps of firstly, using graphene oxide as the growth factor carrier, wrapping growth factors in composite nano granules formed by polycations and polyanions, reacting the nano granules containing the growth factors with the carboxylated graphene oxide so as to form amido bonds, thereby fixing the nano granules on the graphene oxide; and enabling the graphene oxide carrying the growth factors to form the coating on a biologic medical metal surface which is subjected to dopamine treatment by using an extraction method. By utilizing the preparation method, higher growth factor carrying quantity is realized, and meanwhile, the activity of the factors is protected, and the slow-releasing of the factors is realized.

Description

technical field [0001] The invention relates to a preparation method of a slow-release growth factor coating on a medical metal surface. Background technique [0002] Medical metals are widely used in plastic surgery, bone implants and other fields due to their good biocompatibility and excellent mechanical properties. However, medical metals are usually biologically inert, and their surface bone regeneration ability is weak, and new bone growth is slow. As a result, after the medical metal is implanted in the body, it cannot quickly form a firm bond with the surrounding bone tissue. In order to improve the integration between medical metal implants and bone tissue, surface modification is often required. One of the surface modification methods is to load cell growth factors on the surface of medical metal implants to promote the regeneration of bone tissue. Common cell growth factors, such as bone morphogenetic protein (BMP-2), vascular growth factor (VEGF), transforming...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/30
Inventor 鲁雄韩璐王振铭姜丽丽郭亚楠孙洪龙冯波翁杰
Owner SOUTHWEST JIAOTONG UNIV
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