Antihypertensive peptides prepared by ultrasonic-assisted flour weevil larva proteolysis and preparation method thereof
A proteolysis and blood pressure-lowering peptide technology, applied in the field of bioengineering, can solve the problems of low activity and purity, poor absorption of macromolecular polypeptides and proteins, etc., achieve the effects of wide sources, shorten the time of enzymatic hydrolysis, and improve the utilization rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0062] 1. Ultrasonic pretreatment of protein from Tenebrio molitor larvae
[0063] The dried Tenebrio molitor powder was subjected to supercritical CO 2 Degreasing, degreasing conditions: extraction pressure 35 MPa, extraction temperature 35 ℃, extraction time 200 min, normal pressure separation. Take defatted Tenebrio molitor larvae powder (supercritical CO 2 After degreasing, the total protein content is 60.5%) 50 g, add distilled water to prepare a 2% (w / v) solution, and use a pulsed ultrasonic wave with a power of 250 W to treat the defatted Tenebrio molitor larvae protein solution for 30 min, corresponding to a pulsed ultrasonic wave for 2 s, gap time 2 s.
[0064] Preparation of enzymatic hydrolyzate of Tenebrio molitor larvae
[0065] Put the solution in a constant temperature water bath to 60 °C, adjust its pH to 7.5, add 1.2 mL of alkaline protease for enzymolysis, stop the reaction when the degree of hydrolysis reaches 20%, keep stirring the reaction system du...
Embodiment 2
[0071] 1. Sonication Pretreatment of Alkaline Protease
[0072] Take 5 mL of alkaline protease and dilute it to 50 mL with pH 8.0 phosphate buffer solution (concentration: 0.05 mol / L), and process the alkaline protease solution for 30 s with a pulse ultrasonic wave with a power of 50 W. s, gap time 2 s.
[0073] Preparation of enzymatic hydrolyzate of Tenebrio molitor larvae
[0074] The dried Tenebrio molitor powder was subjected to supercritical CO 2 Degreasing, degreasing conditions: extraction pressure 35 MPa, extraction temperature 35 ℃, extraction time 200 min, normal pressure separation. Take defatted Tenebrio molitor larvae powder (supercritical CO 2 After defatting, the total protein content is 60.5%) 50 g, add distilled water to prepare a 4% (m / v) solution, put the solution in a constant temperature water bath to 50 °C, and adjust its pH to 8.0, add 6 mL of ultrasonic pretreated alkaline Protease was used for enzymatic hydrolysis, and the reaction was stopped...
Embodiment 3
[0080] 1. Ultrasonic pretreatment of protein from Tenebrio molitor larvae
[0081] The dried Tenebrio molitor powder was subjected to supercritical CO 2 Degreasing, degreasing conditions: extraction pressure 35 MPa, extraction temperature 35 ℃, extraction time 200 min, normal pressure separation. Take defatted Tenebrio molitor larvae powder (supercritical CO 2 After degreasing, the total protein content is 60.5%) 50 g, add distilled water to prepare a 6% (w / v) solution, and use a pulsed ultrasonic wave with a power of 1250 W to treat the defatted Tenebrio molitor larvae protein solution for 5 min, corresponding to the time of one pulsed ultrasonic wave for 4 s, gap time 2 s.
[0082] Ultrasonic pretreatment of alkaline protease
[0083] Take 5 mL of alkaline protease and dilute it to 50 mL with pH 8.0 phosphate buffer solution (concentration: 0.05 mol / L), and use a pulsed ultrasonic wave with a power of 300 W to treat the alkaline protease solution for 10 s. s, gap tim...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Molecular weight | aaaaa | aaaaa |
| Molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More